Matches in SemOpenAlex for { <https://semopenalex.org/work/W2121808115> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2121808115 endingPage "4788" @default.
- W2121808115 startingPage "4780" @default.
- W2121808115 abstract "Gamma phage specifically lyses vegetative cells of Bacillus anthracis and serves as part of the basis for identification of isolates from agar cultures. We report our study to standardize gamma phage production and preparation and to validate the assay for routine use. Unstable phage preparations were largely reduced through propagation of phage on blood agar cultures of the avirulent B. anthracis strain CDC684 and were adequately stable for extended storage beyond 1 to 2 years at 4 degrees C, provided that the preparation initially gave rise to clearly discernible plaques (macroplaques, 5 to 10 mm in diameter) on dilution at 1:8 or greater during potency testing with the Sterne strain or its equivalent. The primary intent of the assay was to test nonhemolytic, ground-glass-appearing bacterial B. anthracis-like colonies arising from culture of clinical or nonclinical samples on 5% sheep blood agar. Specifically, the assay was designed to show clear or primarily clear circular zones of lysis on bacterial lawns at the site of gamma phage inoculation after incubation at 35 degrees C +/- 2 degrees C for 20 h. When tested with 51 B. anthracis strains and 49 similar non-B. anthracis Bacillus species, the analytical specificity was >95%, a value that is intentionally low because our study design included two rare nonsusceptible B. anthracis strains as well as a rare susceptible non-B. anthracis strain, B. cereus ATCC 4342. Repeatability, day-to-day precision, and analyst-to-analyst precision were superior. The assay was rugged to variations among phage lots, phage concentration, amounts of bacterial inoculum, and incubation times as short as 6 to 8 h. System suitability evaluation showed improved robustness when bacterial lawns were tested with high- and low-density inoculum using the first and second quadrants of a serial four-quadrant streak on 5% sheep blood agar plates." @default.
- W2121808115 created "2016-06-24" @default.
- W2121808115 creator A5016543524 @default.
- W2121808115 creator A5043649226 @default.
- W2121808115 creator A5055188542 @default.
- W2121808115 date "2005-09-01" @default.
- W2121808115 modified "2023-10-18" @default.
- W2121808115 title "Production and Validation of the Use of Gamma Phage for Identification of <i>Bacillus anthracis</i>" @default.
- W2121808115 cites W1574420400 @default.
- W2121808115 cites W1636860196 @default.
- W2121808115 cites W1897619192 @default.
- W2121808115 cites W1970532969 @default.
- W2121808115 cites W1981706059 @default.
- W2121808115 cites W2009334449 @default.
- W2121808115 cites W2424706927 @default.
- W2121808115 cites W2768636159 @default.
- W2121808115 cites W54135030 @default.
- W2121808115 doi "https://doi.org/10.1128/jcm.43.9.4780-4788.2005" @default.
- W2121808115 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1234045" @default.
- W2121808115 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16145141" @default.
- W2121808115 hasPublicationYear "2005" @default.
- W2121808115 type Work @default.
- W2121808115 sameAs 2121808115 @default.
- W2121808115 citedByCount "82" @default.
- W2121808115 countsByYear W21218081152012 @default.
- W2121808115 countsByYear W21218081152013 @default.
- W2121808115 countsByYear W21218081152014 @default.
- W2121808115 countsByYear W21218081152015 @default.
- W2121808115 countsByYear W21218081152016 @default.
- W2121808115 countsByYear W21218081152017 @default.
- W2121808115 countsByYear W21218081152018 @default.
- W2121808115 countsByYear W21218081152019 @default.
- W2121808115 countsByYear W21218081152020 @default.
- W2121808115 countsByYear W21218081152021 @default.
- W2121808115 countsByYear W21218081152022 @default.
- W2121808115 countsByYear W21218081152023 @default.
- W2121808115 crossrefType "journal-article" @default.
- W2121808115 hasAuthorship W2121808115A5016543524 @default.
- W2121808115 hasAuthorship W2121808115A5043649226 @default.
- W2121808115 hasAuthorship W2121808115A5055188542 @default.
- W2121808115 hasBestOaLocation W21218081151 @default.
- W2121808115 hasConcept C105702510 @default.
- W2121808115 hasConcept C153911025 @default.
- W2121808115 hasConcept C2777118263 @default.
- W2121808115 hasConcept C2778022156 @default.
- W2121808115 hasConcept C2778660310 @default.
- W2121808115 hasConcept C2779150676 @default.
- W2121808115 hasConcept C2780239966 @default.
- W2121808115 hasConcept C523546767 @default.
- W2121808115 hasConcept C54355233 @default.
- W2121808115 hasConcept C57409179 @default.
- W2121808115 hasConcept C62643968 @default.
- W2121808115 hasConcept C86803240 @default.
- W2121808115 hasConcept C89423630 @default.
- W2121808115 hasConceptScore W2121808115C105702510 @default.
- W2121808115 hasConceptScore W2121808115C153911025 @default.
- W2121808115 hasConceptScore W2121808115C2777118263 @default.
- W2121808115 hasConceptScore W2121808115C2778022156 @default.
- W2121808115 hasConceptScore W2121808115C2778660310 @default.
- W2121808115 hasConceptScore W2121808115C2779150676 @default.
- W2121808115 hasConceptScore W2121808115C2780239966 @default.
- W2121808115 hasConceptScore W2121808115C523546767 @default.
- W2121808115 hasConceptScore W2121808115C54355233 @default.
- W2121808115 hasConceptScore W2121808115C57409179 @default.
- W2121808115 hasConceptScore W2121808115C62643968 @default.
- W2121808115 hasConceptScore W2121808115C86803240 @default.
- W2121808115 hasConceptScore W2121808115C89423630 @default.
- W2121808115 hasIssue "9" @default.
- W2121808115 hasLocation W21218081151 @default.
- W2121808115 hasLocation W21218081152 @default.
- W2121808115 hasLocation W21218081153 @default.
- W2121808115 hasLocation W21218081154 @default.
- W2121808115 hasOpenAccess W2121808115 @default.
- W2121808115 hasPrimaryLocation W21218081151 @default.
- W2121808115 hasRelatedWork W1574420400 @default.
- W2121808115 hasRelatedWork W166956482 @default.
- W2121808115 hasRelatedWork W2020134144 @default.
- W2121808115 hasRelatedWork W2123961177 @default.
- W2121808115 hasRelatedWork W2125245499 @default.
- W2121808115 hasRelatedWork W221901354 @default.
- W2121808115 hasRelatedWork W2409105800 @default.
- W2121808115 hasRelatedWork W2619815862 @default.
- W2121808115 hasRelatedWork W4233898599 @default.
- W2121808115 hasRelatedWork W4291901062 @default.
- W2121808115 hasVolume "43" @default.
- W2121808115 isParatext "false" @default.
- W2121808115 isRetracted "false" @default.
- W2121808115 magId "2121808115" @default.
- W2121808115 workType "article" @default.