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- W2121848244 abstract "RATIONALE: Tamarillo (Cyphomandra betacea), a member of the Solanaceae family is consumed worldwide, yet little is known about the allergenic proteins in this fruit. The aim of this study was to identify allergens from Tamarillo. METHODS: A food allergy survey was performed in Palmerston North. Participants were requested to fill out a food allergy questionnaire that included information regarding foods they were allergic to and the symptoms they experienced after consuming these foods. One participant with self-reported allergy to Tamarillo fruit experienced hives within 12-24 hours after eating tamarillo. In order to identify the allergenic proteins, tamarillo extract was resolved on 12.5% SDS-PAGE. The IgE binding proteins were detected by immunoblotting with sera from the tamarillo-allergic subject. Two bands at molecular weights of approximately 26 kDa and 15 kDa were detected. The bands were excised from the gel and amino acid sequences of the tryptic digests were determined by de novo sequencing analysis. RESULTS: Homology search of the peptide sequences revealed maximum similarity of the 15 kDa band to pathogenesis-related protein and the 26 kDa band to subtilisin-like protease. CONCLUSIONS: Two IgE binding proteins from Tamarillo fruit have been identified." @default.
- W2121848244 created "2016-06-24" @default.
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- W2121848244 date "2011-02-01" @default.
- W2121848244 modified "2023-09-23" @default.
- W2121848244 title "Identification of Tamarillo (Cyphomandra betacea) Allergens" @default.
- W2121848244 doi "https://doi.org/10.1016/j.jaci.2010.12.443" @default.
- W2121848244 hasPublicationYear "2011" @default.
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