FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2121896543> ?p ?o ?g. }

• W2121896543 endingPage "1449" @default.
• W2121896543 startingPage "1442" @default.
• W2121896543 abstract "The search for an endogenous ligand for the vanilloid receptor (VR or TRPV1) has led to the identification of N-arachidonyl dopamine (NADA). This study investigates the role of protein kinase C (PKC)-mediated phosphorylation on NADA-induced membrane currents in Xenopus oocytes heterologously expressing TRPV1 and in dorsal root ganglion (DRG) neurons. In basal state, current induced by 10 microM NADA is 5-10% of the current induced by 1 microM capsaicin or protons at pH 5. However, PKC activator, phorbol 12,13-dibutyrate (PDBu) strongly potentiated ( approximately 15-fold) the NADA-induced current. Repeated application of NADA at short intervals potentiated its own response approximately fivefold in a PKC-dependent manner. PKC inhibitor, bisindolylmaleimide (BIM, 500 nM), a mutant TRPV1 (S800A/S502A), and maximal activation of PKC abolished the potentiation induced by repeated application of NADA. As a further confirmation that NADA could stimulate PKC, pretreatment with NADA potentiated the response of protons at pH 5 (approximately 20 fold), which was dramatically reduced in the mutant TRPV1. In DRG neurons, capsaicin (100 nM) induced a approximately 15 mV depolarization and initiated a train of action potentials compared with 1 microM NADA that produced a approximately 5 mV response. Pretreatment with PDBu induced significantly larger depolarization and potentiated NADA-induced current. Furthermore, exposure of NADA to the intracellular surface of the membrane-induced larger currents suggesting inaccessibility to the intracellular binding site might contribute to its weaker action. These results indicate that NADA is a potent agonist of VR when the receptor is in the PKC-mediated phosphorylation state." @default.
• W2121896543 created "2016-06-24" @default.
• W2121896543 creator A5031243187 @default.
• W2121896543 creator A5032591220 @default.
• W2121896543 creator A5061425736 @default.
• W2121896543 creator A5087616224 @default.
• W2121896543 date "2004-03-01" @default.
• W2121896543 modified "2023-10-16" @default.
• W2121896543 title "Enhancement of Potency and Efficacy of NADA by PKC-Mediated Phosphorylation of Vanilloid Receptor" @default.
• W2121896543 cites W1492591187 @default.
• W2121896543 cites W1511198581 @default.
• W2121896543 cites W1564453175 @default.
• W2121896543 cites W1569255175 @default.
• W2121896543 cites W1571969265 @default.
• W2121896543 cites W1971566600 @default.
• W2121896543 cites W1985030046 @default.
• W2121896543 cites W1986235007 @default.
• W2121896543 cites W1988375390 @default.
• W2121896543 cites W1995705528 @default.
• W2121896543 cites W2003386605 @default.
• W2121896543 cites W2008522439 @default.
• W2121896543 cites W2015913423 @default.
• W2121896543 cites W2020224671 @default.
• W2121896543 cites W2022819833 @default.
• W2121896543 cites W2029993965 @default.
• W2121896543 cites W2043271992 @default.
• W2121896543 cites W2044632947 @default.
• W2121896543 cites W2047754566 @default.
• W2121896543 cites W2049100687 @default.
• W2121896543 cites W2050781058 @default.
• W2121896543 cites W2055332619 @default.
• W2121896543 cites W2062606765 @default.
• W2121896543 cites W2062671000 @default.
• W2121896543 cites W2068792957 @default.
• W2121896543 cites W2069460649 @default.
• W2121896543 cites W2077176830 @default.
• W2121896543 cites W2078980646 @default.
• W2121896543 cites W2081044920 @default.
• W2121896543 cites W2085802434 @default.
• W2121896543 cites W2088764206 @default.
• W2121896543 cites W2107705149 @default.
• W2121896543 cites W2109293109 @default.
• W2121896543 cites W2119832782 @default.
• W2121896543 cites W2134742719 @default.
• W2121896543 cites W2142664530 @default.
• W2121896543 cites W2146883061 @default.
• W2121896543 cites W2147672258 @default.
• W2121896543 cites W2171717653 @default.
• W2121896543 cites W2195049256 @default.
• W2121896543 cites W2343236095 @default.
• W2121896543 cites W4211202477 @default.
• W2121896543 cites W4230889514 @default.
• W2121896543 doi "https://doi.org/10.1152/jn.00745.2003" @default.
• W2121896543 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14973326" @default.
• W2121896543 hasPublicationYear "2004" @default.
• W2121896543 type Work @default.
• W2121896543 sameAs 2121896543 @default.
• W2121896543 citedByCount "66" @default.
• W2121896543 countsByYear W21218965432012 @default.
• W2121896543 countsByYear W21218965432013 @default.
• W2121896543 countsByYear W21218965432014 @default.
• W2121896543 countsByYear W21218965432015 @default.
• W2121896543 countsByYear W21218965432016 @default.
• W2121896543 countsByYear W21218965432017 @default.
• W2121896543 countsByYear W21218965432018 @default.
• W2121896543 countsByYear W21218965432021 @default.
• W2121896543 crossrefType "journal-article" @default.
• W2121896543 hasAuthorship W2121896543A5031243187 @default.
• W2121896543 hasAuthorship W2121896543A5032591220 @default.
• W2121896543 hasAuthorship W2121896543A5061425736 @default.
• W2121896543 hasAuthorship W2121896543A5087616224 @default.
• W2121896543 hasConcept C11960822 @default.
• W2121896543 hasConcept C12554922 @default.
• W2121896543 hasConcept C126322002 @default.
• W2121896543 hasConcept C134018914 @default.
• W2121896543 hasConcept C155164980 @default.
• W2121896543 hasConcept C160145004 @default.
• W2121896543 hasConcept C169760540 @default.
• W2121896543 hasConcept C170493617 @default.
• W2121896543 hasConcept C185592680 @default.
• W2121896543 hasConcept C195794163 @default.
• W2121896543 hasConcept C25274449 @default.
• W2121896543 hasConcept C2776281502 @default.
• W2121896543 hasConcept C2776354303 @default.
• W2121896543 hasConcept C2777314051 @default.
• W2121896543 hasConcept C2778938600 @default.
• W2121896543 hasConcept C4141045 @default.
• W2121896543 hasConcept C55493867 @default.
• W2121896543 hasConcept C71924100 @default.
• W2121896543 hasConcept C79879829 @default.
• W2121896543 hasConcept C86803240 @default.
• W2121896543 hasConcept C94487597 @default.
• W2121896543 hasConcept C98274493 @default.
• W2121896543 hasConceptScore W2121896543C11960822 @default.
• W2121896543 hasConceptScore W2121896543C12554922 @default.
• W2121896543 hasConceptScore W2121896543C126322002 @default.
• W2121896543 hasConceptScore W2121896543C134018914 @default.
• W2121896543 hasConceptScore W2121896543C155164980 @default.

• next