Matches in SemOpenAlex for { <https://semopenalex.org/work/W2122024692> ?p ?o ?g. }
- W2122024692 endingPage "287" @default.
- W2122024692 startingPage "269" @default.
- W2122024692 abstract "The yeast class III phosphoinositide 3-kinase (PI3K) that catalyses production of the lipid signalling molecule, phosphatidylinositol-3-phosphate, is primarily implicated in vesicle-mediated transport and autophagy. In this study, we identified, through a genetic screen, the Candida glabrata CgVPS15 gene, an orthologue of the Saccharomyces cerevisiae PI3K regulatory subunit-encoding open reading frame (ORF) to be required for impairment of phagosomal maturation in human macrophages. We also disrupted catalytic subunit of the C. glabrata PI3K complex, CgVps34, and found it to be pivotal to arrest mature phagolysosome biogenesis. Further, deletion of either CgVPS15 or CgVPS34 rendered C. glabrata cells hyperadherent to epithelial cells and susceptible to the antimicrobial arsenal of primary murine and cultured human macrophages and diverse stresses. Despite no growth retardation at 37°C, Cgvps15Δ and Cgvps34Δ mutants were severely virulence attenuated in mice. We demonstrate that trafficking and/or processing of the vacuolar lumenal hydrolase, carboxypeptidase Y, and the major adhesin, Epa1, rely on PI3K regulatory mechanisms in C. glabrata. By disrupting autophagy-related PI3K complex genes, we show that C. glabrata PI3K-impeded phagolysosomal acidification is primarily owing to its role in cellular trafficking events. Altogether, our findings underscore the essentiality of PI3K signalling in modulation of host immune response, intracellular survival and virulence in C. glabrata." @default.
- W2122024692 created "2016-06-24" @default.
- W2122024692 creator A5002737277 @default.
- W2122024692 creator A5019316856 @default.
- W2122024692 creator A5042207462 @default.
- W2122024692 creator A5081072671 @default.
- W2122024692 date "2014-10-31" @default.
- W2122024692 modified "2023-09-25" @default.
- W2122024692 title "An essential role for phosphatidylinositol 3-kinase in the inhibition of phagosomal maturation, intracellular survival and virulence in<i>C</i><i>andida glabrata</i>" @default.
- W2122024692 cites W1501109059 @default.
- W2122024692 cites W1647692986 @default.
- W2122024692 cites W1881372541 @default.
- W2122024692 cites W1923816168 @default.
- W2122024692 cites W1967085696 @default.
- W2122024692 cites W1981261463 @default.
- W2122024692 cites W1982281067 @default.
- W2122024692 cites W1985606277 @default.
- W2122024692 cites W2002836781 @default.
- W2122024692 cites W2013131355 @default.
- W2122024692 cites W2026489312 @default.
- W2122024692 cites W2041143337 @default.
- W2122024692 cites W2045508653 @default.
- W2122024692 cites W2054868502 @default.
- W2122024692 cites W2069172362 @default.
- W2122024692 cites W2070667133 @default.
- W2122024692 cites W2075016274 @default.
- W2122024692 cites W2080779226 @default.
- W2122024692 cites W2089894637 @default.
- W2122024692 cites W2096452928 @default.
- W2122024692 cites W2098123098 @default.
- W2122024692 cites W2100246797 @default.
- W2122024692 cites W2103475644 @default.
- W2122024692 cites W2106754534 @default.
- W2122024692 cites W2108543631 @default.
- W2122024692 cites W2111715441 @default.
- W2122024692 cites W2117231363 @default.
- W2122024692 cites W2120824097 @default.
- W2122024692 cites W2122716163 @default.
- W2122024692 cites W2124863746 @default.
- W2122024692 cites W2126734055 @default.
- W2122024692 cites W2133189384 @default.
- W2122024692 cites W2136722399 @default.
- W2122024692 cites W2139297116 @default.
- W2122024692 cites W2143839871 @default.
- W2122024692 cites W2154445113 @default.
- W2122024692 cites W2411891289 @default.
- W2122024692 doi "https://doi.org/10.1111/cmi.12364" @default.
- W2122024692 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25223215" @default.
- W2122024692 hasPublicationYear "2014" @default.
- W2122024692 type Work @default.
- W2122024692 sameAs 2122024692 @default.
- W2122024692 citedByCount "20" @default.
- W2122024692 countsByYear W21220246922015 @default.
- W2122024692 countsByYear W21220246922016 @default.
- W2122024692 countsByYear W21220246922017 @default.
- W2122024692 countsByYear W21220246922018 @default.
- W2122024692 countsByYear W21220246922019 @default.
- W2122024692 countsByYear W21220246922020 @default.
- W2122024692 countsByYear W21220246922021 @default.
- W2122024692 countsByYear W21220246922022 @default.
- W2122024692 countsByYear W21220246922023 @default.
- W2122024692 crossrefType "journal-article" @default.
- W2122024692 hasAuthorship W2122024692A5002737277 @default.
- W2122024692 hasAuthorship W2122024692A5019316856 @default.
- W2122024692 hasAuthorship W2122024692A5042207462 @default.
- W2122024692 hasAuthorship W2122024692A5081072671 @default.
- W2122024692 hasBestOaLocation W21220246921 @default.
- W2122024692 hasConcept C104317684 @default.
- W2122024692 hasConcept C143065580 @default.
- W2122024692 hasConcept C144382850 @default.
- W2122024692 hasConcept C153726182 @default.
- W2122024692 hasConcept C161478664 @default.
- W2122024692 hasConcept C184235292 @default.
- W2122024692 hasConcept C190283241 @default.
- W2122024692 hasConcept C203522944 @default.
- W2122024692 hasConcept C207177822 @default.
- W2122024692 hasConcept C2777576037 @default.
- W2122024692 hasConcept C2780610907 @default.
- W2122024692 hasConcept C2780670043 @default.
- W2122024692 hasConcept C2780917455 @default.
- W2122024692 hasConcept C55493867 @default.
- W2122024692 hasConcept C60987743 @default.
- W2122024692 hasConcept C62478195 @default.
- W2122024692 hasConcept C79879829 @default.
- W2122024692 hasConcept C86554907 @default.
- W2122024692 hasConcept C86803240 @default.
- W2122024692 hasConcept C89423630 @default.
- W2122024692 hasConcept C95444343 @default.
- W2122024692 hasConceptScore W2122024692C104317684 @default.
- W2122024692 hasConceptScore W2122024692C143065580 @default.
- W2122024692 hasConceptScore W2122024692C144382850 @default.
- W2122024692 hasConceptScore W2122024692C153726182 @default.
- W2122024692 hasConceptScore W2122024692C161478664 @default.
- W2122024692 hasConceptScore W2122024692C184235292 @default.
- W2122024692 hasConceptScore W2122024692C190283241 @default.
- W2122024692 hasConceptScore W2122024692C203522944 @default.
- W2122024692 hasConceptScore W2122024692C207177822 @default.
- W2122024692 hasConceptScore W2122024692C2777576037 @default.