FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2122126894> ?p ?o ?g. }

• W2122126894 endingPage "475" @default.
• W2122126894 startingPage "465" @default.
• W2122126894 abstract "An optimized culture method for detection of infection of fish with the Red spotted grouper nervous necrosis virus (RGNNV) genotype of betanodavirus in striped snakehead (SSN-1, Channa striatus) cells is described. Inoculation of fish tissue homogenates at the same time or within 4 hr of seeding the SSN-1 cells was as sensitive as the method recommended by the World Organization for Animal Health, where homogenates were adsorbed onto an established cell monolayer. Such modification halved the time required and the costs of consumables, and reduced the potential for error when processing large numbers of samples. Positive culture results were obtained from 88.3% of 392 fish tissue homogenates in which RGNNV was detected using a quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay; 99.7% of 943 tissue homogenates, which were qRT-PCR negative, were cell culture negative. Cytopathic effect (CPE) was characterized by large intracytoplasmic vacuoles in 0.1–60% of cells. Detachment of affected cells from the culture surface resulting in progressive disruption of the monolayer occurred in 46.4% of primary cultures and 96.0% of subcultures of positive samples. Identification of CPE that did not disrupt the cell monolayer increased estimates of the 50% tissue culture infective dose (TCID 50 ) by 1.07–2.79 logs (95% confidence interval). The predicted mean TCID 50 /ml was 3.3 logs higher when cells were inoculated less than 36 hr after subculture at less than 80% confluence compared to cells inoculated at greater than 80% confluence and more than 36 hr after subculture ( P < 0.05)." @default.
• W2122126894 created "2016-06-24" @default.
• W2122126894 creator A5019013219 @default.
• W2122126894 creator A5041398207 @default.
• W2122126894 creator A5086423005 @default.
• W2122126894 date "2011-05-01" @default.
• W2122126894 modified "2023-09-25" @default.
• W2122126894 title "Optimization of Betanodavirus culture and enumeration in striped snakehead fish cells" @default.
• W2122126894 cites W1964501652 @default.
• W2122126894 cites W1965753893 @default.
• W2122126894 cites W1968361789 @default.
• W2122126894 cites W1971216623 @default.
• W2122126894 cites W1972662784 @default.
• W2122126894 cites W1979766121 @default.
• W2122126894 cites W1992309624 @default.
• W2122126894 cites W1993973290 @default.
• W2122126894 cites W1994789521 @default.
• W2122126894 cites W1995276706 @default.
• W2122126894 cites W1998990320 @default.
• W2122126894 cites W2008771562 @default.
• W2122126894 cites W2009434411 @default.
• W2122126894 cites W2010955287 @default.
• W2122126894 cites W2036920662 @default.
• W2122126894 cites W2061612682 @default.
• W2122126894 cites W2073769687 @default.
• W2122126894 cites W2079135770 @default.
• W2122126894 cites W2107214269 @default.
• W2122126894 cites W2111775485 @default.
• W2122126894 cites W2117922752 @default.
• W2122126894 cites W2118444009 @default.
• W2122126894 cites W2122766220 @default.
• W2122126894 cites W2126764708 @default.
• W2122126894 cites W2147062706 @default.
• W2122126894 cites W2150516885 @default.
• W2122126894 cites W2156373156 @default.
• W2122126894 cites W2159782714 @default.
• W2122126894 cites W2162506288 @default.
• W2122126894 cites W2162820949 @default.
• W2122126894 cites W2434254482 @default.
• W2122126894 cites W43569997 @default.
• W2122126894 doi "https://doi.org/10.1177/1040638711404147" @default.
• W2122126894 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21908274" @default.
• W2122126894 hasPublicationYear "2011" @default.
• W2122126894 type Work @default.
• W2122126894 sameAs 2122126894 @default.
• W2122126894 citedByCount "13" @default.
• W2122126894 countsByYear W21221268942012 @default.
• W2122126894 countsByYear W21221268942013 @default.
• W2122126894 countsByYear W21221268942015 @default.
• W2122126894 countsByYear W21221268942016 @default.
• W2122126894 countsByYear W21221268942017 @default.
• W2122126894 countsByYear W21221268942018 @default.
• W2122126894 countsByYear W21221268942020 @default.
• W2122126894 crossrefType "journal-article" @default.
• W2122126894 hasAuthorship W2122126894A5019013219 @default.
• W2122126894 hasAuthorship W2122126894A5041398207 @default.
• W2122126894 hasAuthorship W2122126894A5086423005 @default.
• W2122126894 hasConcept C102568950 @default.
• W2122126894 hasConcept C104317684 @default.
• W2122126894 hasConcept C153911025 @default.
• W2122126894 hasConcept C159047783 @default.
• W2122126894 hasConcept C16685009 @default.
• W2122126894 hasConcept C180032290 @default.
• W2122126894 hasConcept C190062978 @default.
• W2122126894 hasConcept C202751555 @default.
• W2122126894 hasConcept C203014093 @default.
• W2122126894 hasConcept C2522874641 @default.
• W2122126894 hasConcept C2775996393 @default.
• W2122126894 hasConcept C2779502808 @default.
• W2122126894 hasConcept C2909208804 @default.
• W2122126894 hasConcept C48023723 @default.
• W2122126894 hasConcept C505870484 @default.
• W2122126894 hasConcept C54355233 @default.
• W2122126894 hasConcept C55493867 @default.
• W2122126894 hasConcept C71924100 @default.
• W2122126894 hasConcept C81885089 @default.
• W2122126894 hasConcept C86803240 @default.
• W2122126894 hasConcept C87863119 @default.
• W2122126894 hasConcept C89423630 @default.
• W2122126894 hasConcept C90703135 @default.
• W2122126894 hasConceptScore W2122126894C102568950 @default.
• W2122126894 hasConceptScore W2122126894C104317684 @default.
• W2122126894 hasConceptScore W2122126894C153911025 @default.
• W2122126894 hasConceptScore W2122126894C159047783 @default.
• W2122126894 hasConceptScore W2122126894C16685009 @default.
• W2122126894 hasConceptScore W2122126894C180032290 @default.
• W2122126894 hasConceptScore W2122126894C190062978 @default.
• W2122126894 hasConceptScore W2122126894C202751555 @default.
• W2122126894 hasConceptScore W2122126894C203014093 @default.
• W2122126894 hasConceptScore W2122126894C2522874641 @default.
• W2122126894 hasConceptScore W2122126894C2775996393 @default.
• W2122126894 hasConceptScore W2122126894C2779502808 @default.
• W2122126894 hasConceptScore W2122126894C2909208804 @default.
• W2122126894 hasConceptScore W2122126894C48023723 @default.
• W2122126894 hasConceptScore W2122126894C505870484 @default.
• W2122126894 hasConceptScore W2122126894C54355233 @default.
• W2122126894 hasConceptScore W2122126894C55493867 @default.
• W2122126894 hasConceptScore W2122126894C71924100 @default.

• next