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- W212216551 abstract "Some molecular properties of Apple chlorotic leaf spot virus (ACLSV) isolates, infecting fruit trees in collection and commercial orchards in Bulgaria, were studied. Sequence analysis was performed on the coat protein (CP) and movement protein (MP) coding regions for isolates from peach (R2D43, R1D2P-1), sweet cherry (P1R9D9, P1R6D4) and apple trees (P10R1D3), in order to determine the sequence variability of ACLSV isolates. The similarity in the percentage figures of the sequence variability was between 81.1 and 99.8%; the highest being between the isolates RID2P-1 and P1R6D4, and the lowest between the isolates R2D43 and P1R6D4. Sequences of the studied isolates were also compared with those of genome fragments of ACLSV isolates published in the GenBank database. To check the electrophoretic mobility and CP of six isolates of ACLSV, the following were subjected to Western blotting analysis; P1R9D9, P1R6D4, from sweet cherry, and R2D41, R2D43, R2D49, R1D2P-1 from peach. Two different migration rates were found for the CP of these isolates. The coat protein of the R1D2P-1 isolate migrated slower than the CP of other isolates." @default.
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- W212216551 date "2007-01-01" @default.
- W212216551 modified "2023-09-28" @default.
- W212216551 title "Some molecular properties of several isolates of apple chlorotic leaf spot virus from Bulgaria" @default.
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