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- W2122231876 abstract "An alcohol dehydrogenase (ADH) null mutant of Drosophila melanogaster (AdhnLA405) originally recovered following X-ray irradiation of mature sperm (Aaron, 979) is analyzed by Southern blotting, Western blotting, and DNA sequencing. The genetic, immunologic, and nucleic acid sequence data are consistent with the hypothesis that a cross-over event, independent of X-irradiation, between parental chromosomes is responsible for the ADH null phenotype of AdhnLA405. By DNA-sequence analysis we show that molecular cloning of this locus (i.e., propagation in prokaryotic hosts) apparently does not introduce any spurious changes (substitutions, additions, deletions, or rearrangements) within the DNA." @default.
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- W2122231876 date "1988-02-01" @default.
- W2122231876 modified "2023-09-25" @default.
- W2122231876 title "Molecular analysis of Drosophila melanogasterAdhnLA405 confirms reliability of DNA-sequencing methodology" @default.
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- W2122231876 doi "https://doi.org/10.1016/0165-7992(88)90041-3" @default.
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