FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2122796688> ?p ?o ?g. }

• W2122796688 endingPage "951" @default.
• W2122796688 startingPage "941" @default.
• W2122796688 abstract "S-Alk(en)yl-l-cysteine sulfoxides are cysteine-derived secondary metabolites highly accumulated in the genus Allium. Despite pharmaceutical importance, the enzymes that contribute to the biosynthesis of S-alk-(en)yl-l-cysteine sulfoxides in Allium plants remain largely unknown. Here, we report the identification of a flavin-containing monooxygenase, AsFMO1, in garlic (Allium sativum), which is responsible for the S-oxygenation reaction in the biosynthesis of S-allyl-l-cysteine sulfoxide (alliin). Recombinant AsFMO1 protein catalyzed the stereoselective S-oxygenation of S-allyl-l-cysteine to nearly exclusively yield (RC SS )-S-allylcysteine sulfoxide, which has identical stereochemistry to the major natural form of alliin in garlic. The S-oxygenation reaction catalyzed by AsFMO1 was dependent on the presence of nicotinamide adenine dinucleotide phosphate (NADPH) and flavin adenine dinucleotide (FAD), consistent with other known flavin-containing monooxygenases. AsFMO1 preferred S-allyl-l-cysteine to γ-glutamyl-S-allyl-l-cysteine as the S-oxygenation substrate, suggesting that in garlic, the S-oxygenation of alliin biosynthetic intermediates primarily occurs after deglutamylation. The transient expression of green fluorescent protein (GFP) fusion proteins indicated that AsFMO1 is localized in the cytosol. AsFMO1 mRNA was accumulated in storage leaves of pre-emergent nearly sprouting bulbs, and in various tissues of sprouted bulbs with green foliage leaves. Taken together, our results suggest that AsFMO1 functions as an S-allyl-l-cysteine S-oxygenase, and contributes to the production of alliin both through the conversion of stored γ-glutamyl-S-allyl-l-cysteine to alliin in storage leaves during sprouting and through the de novo biosynthesis of alliin in green foliage leaves." @default.
• W2122796688 created "2016-06-24" @default.
• W2122796688 creator A5011990657 @default.
• W2122796688 creator A5044036694 @default.
• W2122796688 creator A5044293644 @default.
• W2122796688 creator A5052696406 @default.
• W2122796688 creator A5056985815 @default.
• W2122796688 creator A5059987110 @default.
• W2122796688 creator A5062477534 @default.
• W2122796688 creator A5079802057 @default.
• W2122796688 creator A5082191180 @default.
• W2122796688 date "2015-09-01" @default.
• W2122796688 modified "2023-09-27" @default.
• W2122796688 title "Identification of a flavin-containing<i>S</i>-oxygenating monooxygenase involved in alliin biosynthesis in garlic" @default.
• W2122796688 cites W1561924357 @default.
• W2122796688 cites W1967298953 @default.
• W2122796688 cites W1968496736 @default.
• W2122796688 cites W1970030667 @default.
• W2122796688 cites W1971665205 @default.
• W2122796688 cites W1989184123 @default.
• W2122796688 cites W1998689715 @default.
• W2122796688 cites W2000678781 @default.
• W2122796688 cites W2019929700 @default.
• W2122796688 cites W2020954114 @default.
• W2122796688 cites W2022475786 @default.
• W2122796688 cites W2027305850 @default.
• W2122796688 cites W2036529190 @default.
• W2122796688 cites W2044221942 @default.
• W2122796688 cites W2055370635 @default.
• W2122796688 cites W2077000688 @default.
• W2122796688 cites W2083657676 @default.
• W2122796688 cites W2084025265 @default.
• W2122796688 cites W2086437716 @default.
• W2122796688 cites W2086660631 @default.
• W2122796688 cites W2087943237 @default.
• W2122796688 cites W2095209109 @default.
• W2122796688 cites W2098655584 @default.
• W2122796688 cites W2098998842 @default.
• W2122796688 cites W2102279554 @default.
• W2122796688 cites W2117719228 @default.
• W2122796688 cites W2122442193 @default.
• W2122796688 cites W2124060569 @default.
• W2122796688 cites W2134844199 @default.
• W2122796688 cites W2145933997 @default.
• W2122796688 cites W2150943030 @default.
• W2122796688 cites W2152207030 @default.
• W2122796688 cites W2155201524 @default.
• W2122796688 cites W2165296968 @default.
• W2122796688 cites W2314348761 @default.
• W2122796688 cites W2334963700 @default.
• W2122796688 cites W2422931415 @default.
• W2122796688 cites W4293247451 @default.
• W2122796688 doi "https://doi.org/10.1111/tpj.12954" @default.
• W2122796688 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26345717" @default.
• W2122796688 hasPublicationYear "2015" @default.
• W2122796688 type Work @default.
• W2122796688 sameAs 2122796688 @default.
• W2122796688 citedByCount "54" @default.
• W2122796688 countsByYear W21227966882017 @default.
• W2122796688 countsByYear W21227966882018 @default.
• W2122796688 countsByYear W21227966882019 @default.
• W2122796688 countsByYear W21227966882020 @default.
• W2122796688 countsByYear W21227966882021 @default.
• W2122796688 countsByYear W21227966882022 @default.
• W2122796688 countsByYear W21227966882023 @default.
• W2122796688 crossrefType "journal-article" @default.
• W2122796688 hasAuthorship W2122796688A5011990657 @default.
• W2122796688 hasAuthorship W2122796688A5044036694 @default.
• W2122796688 hasAuthorship W2122796688A5044293644 @default.
• W2122796688 hasAuthorship W2122796688A5052696406 @default.
• W2122796688 hasAuthorship W2122796688A5056985815 @default.
• W2122796688 hasAuthorship W2122796688A5059987110 @default.
• W2122796688 hasAuthorship W2122796688A5062477534 @default.
• W2122796688 hasAuthorship W2122796688A5079802057 @default.
• W2122796688 hasAuthorship W2122796688A5082191180 @default.
• W2122796688 hasBestOaLocation W21227966881 @default.
• W2122796688 hasConcept C13002179 @default.
• W2122796688 hasConcept C178790620 @default.
• W2122796688 hasConcept C181199279 @default.
• W2122796688 hasConcept C185592680 @default.
• W2122796688 hasConcept C197957613 @default.
• W2122796688 hasConcept C2775859393 @default.
• W2122796688 hasConcept C2776517821 @default.
• W2122796688 hasConcept C2778448320 @default.
• W2122796688 hasConcept C2779201268 @default.
• W2122796688 hasConcept C2781005338 @default.
• W2122796688 hasConcept C2991964360 @default.
• W2122796688 hasConcept C526171541 @default.
• W2122796688 hasConcept C553450214 @default.
• W2122796688 hasConcept C55493867 @default.
• W2122796688 hasConcept C59822182 @default.
• W2122796688 hasConcept C86803240 @default.
• W2122796688 hasConceptScore W2122796688C13002179 @default.
• W2122796688 hasConceptScore W2122796688C178790620 @default.
• W2122796688 hasConceptScore W2122796688C181199279 @default.
• W2122796688 hasConceptScore W2122796688C185592680 @default.
• W2122796688 hasConceptScore W2122796688C197957613 @default.
• W2122796688 hasConceptScore W2122796688C2775859393 @default.

• next