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- W2122936811 abstract "High-throughput DNA sequencing development for mutation screening and identification is essential to realize the goal of pharmacogenomics and personalized medicine, which will lead to a new era in clinical medicine and healthcare. Molecular engineering approaches to modify the building blocks of DNA by introducing functional groups for purification and detection has led to the development of high-throughput genetic analysis technologies. This review is focused on the following two DNA sequencing approaches. The first approach is based on the use of molecular affinity and mass spectrometry to perform quick and highly accurate mutation screening, heterozygote identification and insertion/deletion detection. The second approach is based on a sequencing-by-synthesis platform that has the potential for generating DNA sequencing data in a massive, parallel manner. The basic principles, fundamental challenges and methods of implementation of these exciting new technologies will be discussed." @default.
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- W2122936811 date "2005-09-01" @default.
- W2122936811 modified "2023-10-10" @default.
- W2122936811 title "Molecular engineering approaches for DNA sequencing and analysis" @default.
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- W2122936811 doi "https://doi.org/10.1586/14737159.5.5.797" @default.
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