Matches in SemOpenAlex for { <https://semopenalex.org/work/W2122982012> ?p ?o ?g. }
- W2122982012 endingPage "239" @default.
- W2122982012 startingPage "231" @default.
- W2122982012 abstract "Annotation of protein-coding genes is a key goal of genome sequencing projects. In spite of tremendous recent advances in computational gene finding, comprehensive annotation remains a challenge. Peptide mass spectrometry is a powerful tool for researching the dynamic proteome and suggests an attractive approach to discover and validate protein-coding genes. We present algorithms to construct and efficiently search spectra against a genomic database, with no prior knowledge of encoded proteins. By searching a corpus of 18.5 million tandem mass spectra (MS/MS) from human proteomic samples, we validate 39,000 exons and 11,000 introns at the level of translation. We present translation-level evidence for novel or extended exons in 16 genes, confirm translation of 224 hypothetical proteins, and discover or confirm over 40 alternative splicing events. Polymorphisms are efficiently encoded in our database, allowing us to observe variant alleles for 308 coding SNPs. Finally, we demonstrate the use of mass spectrometry to improve automated gene prediction, adding 800 correct exons to our predictions using a simple rescoring strategy. Our results demonstrate that proteomic profiling should play a role in any genome sequencing project." @default.
- W2122982012 created "2016-06-24" @default.
- W2122982012 creator A5008832336 @default.
- W2122982012 creator A5012296563 @default.
- W2122982012 creator A5028801735 @default.
- W2122982012 creator A5055764938 @default.
- W2122982012 creator A5063689808 @default.
- W2122982012 creator A5065946017 @default.
- W2122982012 creator A5067701434 @default.
- W2122982012 date "2006-12-22" @default.
- W2122982012 modified "2023-10-08" @default.
- W2122982012 title "Improving gene annotation using peptide mass spectrometry" @default.
- W2122982012 cites W1557336618 @default.
- W2122982012 cites W1977184198 @default.
- W2122982012 cites W1981593008 @default.
- W2122982012 cites W1987445292 @default.
- W2122982012 cites W1995454576 @default.
- W2122982012 cites W1996600592 @default.
- W2122982012 cites W2002519661 @default.
- W2122982012 cites W2005716310 @default.
- W2122982012 cites W2023096047 @default.
- W2122982012 cites W2024141877 @default.
- W2122982012 cites W2025075986 @default.
- W2122982012 cites W2031077023 @default.
- W2122982012 cites W2045985536 @default.
- W2122982012 cites W2048453120 @default.
- W2122982012 cites W2051795211 @default.
- W2122982012 cites W2051978415 @default.
- W2122982012 cites W2059572899 @default.
- W2122982012 cites W2075036506 @default.
- W2122982012 cites W2075837782 @default.
- W2122982012 cites W2077689651 @default.
- W2122982012 cites W2083932946 @default.
- W2122982012 cites W2096721473 @default.
- W2122982012 cites W2097694304 @default.
- W2122982012 cites W2097847369 @default.
- W2122982012 cites W2099964107 @default.
- W2122982012 cites W2103619713 @default.
- W2122982012 cites W2107903949 @default.
- W2122982012 cites W2112078820 @default.
- W2122982012 cites W2126362155 @default.
- W2122982012 cites W2129895984 @default.
- W2122982012 cites W2131074700 @default.
- W2122982012 cites W2131283958 @default.
- W2122982012 cites W2131362052 @default.
- W2122982012 cites W2139455449 @default.
- W2122982012 cites W2142418779 @default.
- W2122982012 cites W2143345980 @default.
- W2122982012 cites W2143447881 @default.
- W2122982012 cites W2150746288 @default.
- W2122982012 cites W2151450179 @default.
- W2122982012 cites W2151464048 @default.
- W2122982012 cites W2153167812 @default.
- W2122982012 cites W2163078532 @default.
- W2122982012 cites W2247766769 @default.
- W2122982012 cites W3027636881 @default.
- W2122982012 cites W4230932396 @default.
- W2122982012 cites W4238323524 @default.
- W2122982012 cites W4371640544 @default.
- W2122982012 doi "https://doi.org/10.1101/gr.5646507" @default.
- W2122982012 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1781355" @default.
- W2122982012 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17189379" @default.
- W2122982012 hasPublicationYear "2006" @default.
- W2122982012 type Work @default.
- W2122982012 sameAs 2122982012 @default.
- W2122982012 citedByCount "168" @default.
- W2122982012 countsByYear W21229820122012 @default.
- W2122982012 countsByYear W21229820122013 @default.
- W2122982012 countsByYear W21229820122014 @default.
- W2122982012 countsByYear W21229820122015 @default.
- W2122982012 countsByYear W21229820122016 @default.
- W2122982012 countsByYear W21229820122017 @default.
- W2122982012 countsByYear W21229820122018 @default.
- W2122982012 countsByYear W21229820122019 @default.
- W2122982012 countsByYear W21229820122020 @default.
- W2122982012 countsByYear W21229820122021 @default.
- W2122982012 countsByYear W21229820122022 @default.
- W2122982012 crossrefType "journal-article" @default.
- W2122982012 hasAuthorship W2122982012A5008832336 @default.
- W2122982012 hasAuthorship W2122982012A5012296563 @default.
- W2122982012 hasAuthorship W2122982012A5028801735 @default.
- W2122982012 hasAuthorship W2122982012A5055764938 @default.
- W2122982012 hasAuthorship W2122982012A5063689808 @default.
- W2122982012 hasAuthorship W2122982012A5065946017 @default.
- W2122982012 hasAuthorship W2122982012A5067701434 @default.
- W2122982012 hasBestOaLocation W21229820121 @default.
- W2122982012 hasConcept C104317684 @default.
- W2122982012 hasConcept C104397665 @default.
- W2122982012 hasConcept C105565629 @default.
- W2122982012 hasConcept C141231307 @default.
- W2122982012 hasConcept C145741570 @default.
- W2122982012 hasConcept C189206191 @default.
- W2122982012 hasConcept C194583182 @default.
- W2122982012 hasConcept C197077220 @default.
- W2122982012 hasConcept C2776321320 @default.
- W2122982012 hasConcept C2908923196 @default.
- W2122982012 hasConcept C36823959 @default.
- W2122982012 hasConcept C46111723 @default.