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- W2123042457 abstract "Abstract The nuclear pore complex (NPC) facilitates nucleocytoplasmic transport, a crucial process for various cellular activities. The NPC comprises ~30 nucleoporins and is well characterized in vertebrates and yeast. However, only eight plant nucleoporins have been identified, and little information is available about the complete molecular structure of plant NPCs. In this study, an interactive proteomic approach was used to identify Arabidopsis thaliana nucleoporins. A series of five cycles of interactive proteomic analysis was performed using green fluorescent protein (GFP)-tagged nucleoporins. The identified nucleoporins were then cloned and subcellular localization analyses were performed. We found that the plant NPC contains at least 30 nucleoporins, 22 of which had not been previously annotated. Surprisingly, plant nucleoporins shared a similar domain organization to their vertebrate (human) and yeast (Saccharomyces cerevisiae) counterparts. Moreover, the plant nucleoporins exhibited higher sequence homology to vertebrate nucleoporins than to yeast nucleoporins. Plant NPCs lacked seven components (NUCLEOPORIN358 [Nup358], Nup188, Nup153, Nup45, Nup37, NUCLEAR DIVISION CYCLE1, and PORE MEMBRANE PROTEIN OF 121 kD) that were present in vertebrate NPCs. However, plants possessed a nucleoporin, Nup136/Nup1, that contained Phe-Gly repeats, and sequence analysis failed to identify a vertebrate homolog for this protein. Interestingly, Nup136-GFP showed greater mobility on the nuclear envelope than did other nucleoporins, and a Nup136/Nup1 deficiency caused various defects in plant development. These findings provide valuable new information about plant NPC structure and function." @default.
- W2123042457 created "2016-06-24" @default.
- W2123042457 creator A5034791415 @default.
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- W2123042457 creator A5085284499 @default.
- W2123042457 creator A5091102988 @default.
- W2123042457 date "2010-12-01" @default.
- W2123042457 modified "2023-10-12" @default.
- W2123042457 title "Identification and Characterization of Nuclear Pore Complex Components in<i>Arabidopsis thaliana</i> " @default.
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- W2123042457 doi "https://doi.org/10.1105/tpc.110.079947" @default.
- W2123042457 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3027183" @default.
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