Matches in SemOpenAlex for { <https://semopenalex.org/work/W2123102279> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2123102279 endingPage "327" @default.
- W2123102279 startingPage "312" @default.
- W2123102279 abstract "Shewanella oneidensis MR-1 is a Gram-negative, nonfermentative rod with a complex electron transport system which facilitates its ability to use a variety of terminal electron acceptors, including fumarate, for anaerobic respiration. CMTn-3, a mutant isolated by transposon (TnphoA) mutagenesis, can no longer use fumarate as an electron acceptor; it lacks fumarate reductase activity as well as a 65-kDa soluble tetraheme flavocytochrome c. The sequence of the TnphoA-flanking genomic DNA of CMTn-3 did not align to those for fumarate reductase or related electron transport genes from other bacteria. Sequence analysis of the MR-1 genomic database demonstrated that an open reading frame encoding a 65-kDa tetraheme cytochrome c with sequence similarity to the fumarate reductase from S. frigidimarina NCIMB400 was found 8 kb away from the TnphoA-flanking genomic DNA of CMTn-3. PCR analysis demonstrated that a large deletion (>or=9.2 kb and <or=11 kb) of genomic DNA occurred in CMTn-3 as a result of TnphoA insertion. This deletion included at least half of the fumarate reductase gene as well as approximately 8 kb of upstream DNA. Complementation of CMTn-3 with the fumarate reductase gene plus 0.5-kb of upstream DNA restored growth on fumarate. These studies explicitly define the sole physiological fumarate reductase gene from the several possibilities suggested by the genomic sequence of MR-1. Surprisingly, the fumarate reductase gene plus 0.77-kb upstream DNA from S. frigidimarina NCIMB400 did not complement CMTn-3." @default.
- W2123102279 created "2016-06-24" @default.
- W2123102279 creator A5006361845 @default.
- W2123102279 creator A5021492589 @default.
- W2123102279 creator A5021832087 @default.
- W2123102279 date "2003-08-01" @default.
- W2123102279 modified "2023-10-10" @default.
- W2123102279 title "Identification of the gene encoding the sole physiological fumarate reductase in Shewanella oneidensis MR-1" @default.
- W2123102279 doi "https://doi.org/10.1002/jobm.200390034" @default.
- W2123102279 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12872312" @default.
- W2123102279 hasPublicationYear "2003" @default.
- W2123102279 type Work @default.
- W2123102279 sameAs 2123102279 @default.
- W2123102279 citedByCount "41" @default.
- W2123102279 countsByYear W21231022792012 @default.
- W2123102279 countsByYear W21231022792013 @default.
- W2123102279 countsByYear W21231022792014 @default.
- W2123102279 countsByYear W21231022792015 @default.
- W2123102279 countsByYear W21231022792016 @default.
- W2123102279 countsByYear W21231022792018 @default.
- W2123102279 countsByYear W21231022792019 @default.
- W2123102279 countsByYear W21231022792020 @default.
- W2123102279 countsByYear W21231022792021 @default.
- W2123102279 countsByYear W21231022792022 @default.
- W2123102279 countsByYear W21231022792023 @default.
- W2123102279 crossrefType "journal-article" @default.
- W2123102279 hasAuthorship W2123102279A5006361845 @default.
- W2123102279 hasAuthorship W2123102279A5021492589 @default.
- W2123102279 hasAuthorship W2123102279A5021832087 @default.
- W2123102279 hasConcept C104317684 @default.
- W2123102279 hasConcept C121612415 @default.
- W2123102279 hasConcept C134651460 @default.
- W2123102279 hasConcept C143065580 @default.
- W2123102279 hasConcept C153911025 @default.
- W2123102279 hasConcept C17757408 @default.
- W2123102279 hasConcept C181199279 @default.
- W2123102279 hasConcept C2781170380 @default.
- W2123102279 hasConcept C28693550 @default.
- W2123102279 hasConcept C4918238 @default.
- W2123102279 hasConcept C523546767 @default.
- W2123102279 hasConcept C54355233 @default.
- W2123102279 hasConcept C547475151 @default.
- W2123102279 hasConcept C552990157 @default.
- W2123102279 hasConcept C55493867 @default.
- W2123102279 hasConcept C86803240 @default.
- W2123102279 hasConceptScore W2123102279C104317684 @default.
- W2123102279 hasConceptScore W2123102279C121612415 @default.
- W2123102279 hasConceptScore W2123102279C134651460 @default.
- W2123102279 hasConceptScore W2123102279C143065580 @default.
- W2123102279 hasConceptScore W2123102279C153911025 @default.
- W2123102279 hasConceptScore W2123102279C17757408 @default.
- W2123102279 hasConceptScore W2123102279C181199279 @default.
- W2123102279 hasConceptScore W2123102279C2781170380 @default.
- W2123102279 hasConceptScore W2123102279C28693550 @default.
- W2123102279 hasConceptScore W2123102279C4918238 @default.
- W2123102279 hasConceptScore W2123102279C523546767 @default.
- W2123102279 hasConceptScore W2123102279C54355233 @default.
- W2123102279 hasConceptScore W2123102279C547475151 @default.
- W2123102279 hasConceptScore W2123102279C552990157 @default.
- W2123102279 hasConceptScore W2123102279C55493867 @default.
- W2123102279 hasConceptScore W2123102279C86803240 @default.
- W2123102279 hasIssue "4" @default.
- W2123102279 hasLocation W21231022791 @default.
- W2123102279 hasLocation W21231022792 @default.
- W2123102279 hasOpenAccess W2123102279 @default.
- W2123102279 hasPrimaryLocation W21231022791 @default.
- W2123102279 hasRelatedWork W1991753746 @default.
- W2123102279 hasRelatedWork W2017908005 @default.
- W2123102279 hasRelatedWork W2035409691 @default.
- W2123102279 hasRelatedWork W2054659986 @default.
- W2123102279 hasRelatedWork W2072952539 @default.
- W2123102279 hasRelatedWork W2092510143 @default.
- W2123102279 hasRelatedWork W2138252528 @default.
- W2123102279 hasRelatedWork W2171475414 @default.
- W2123102279 hasRelatedWork W2436039627 @default.
- W2123102279 hasRelatedWork W4245240558 @default.
- W2123102279 hasVolume "43" @default.
- W2123102279 isParatext "false" @default.
- W2123102279 isRetracted "false" @default.
- W2123102279 magId "2123102279" @default.
- W2123102279 workType "article" @default.