Matches in SemOpenAlex for { <https://semopenalex.org/work/W2123144950> ?p ?o ?g. }
- W2123144950 endingPage "683" @default.
- W2123144950 startingPage "672" @default.
- W2123144950 abstract "A large number of proteins in the tonoplast, including pumps, carriers, ion channels and receptors support the various functions of the plant vacuole. To date, few proteins involved in these activities have been identified at the molecular level. In this study, proteomic analysis was used to identify new tonoplast proteins. A primary requirement of any organelle analysis by proteomics is that the purity of the isolated organelle needs to be high. Using suspension-cultured Arabidopsis cells (Arabidopsis Col-0 cell suspension), a method was developed for the isolation of intact highly purified vacuoles. No plasma membrane proteins were detected in Western blots of the isolated vacuole fraction, and only a few proteins from the Golgi and endoplasmic reticulum. The proteomic analysis of the purified tonoplast involved fractionation of the proteins by SDS-PAGE and analysis by LC-MS/MS. Using this approach, it was possible to identify 163 proteins. These included well-characterized tonoplast proteins such as V-type H+-ATPases and V-type H+-PPases, and others with functions reasonably expected to be related to the tonoplast. There were also a number of proteins for which a function has not yet been deduced." @default.
- W2123144950 created "2016-06-24" @default.
- W2123144950 creator A5006947150 @default.
- W2123144950 creator A5034791415 @default.
- W2123144950 creator A5043878996 @default.
- W2123144950 creator A5056591341 @default.
- W2123144950 creator A5060352866 @default.
- W2123144950 creator A5068540369 @default.
- W2123144950 creator A5068950868 @default.
- W2123144950 creator A5069711133 @default.
- W2123144950 creator A5077910250 @default.
- W2123144950 creator A5082565276 @default.
- W2123144950 date "2004-06-15" @default.
- W2123144950 modified "2023-10-17" @default.
- W2123144950 title "Isolation of Intact Vacuoles and Proteomic Analysis of Tonoplast from Suspension-Cultured Cells of Arabidopsis thaliana" @default.
- W2123144950 cites W1494075612 @default.
- W2123144950 cites W1964399324 @default.
- W2123144950 cites W1974472308 @default.
- W2123144950 cites W1983065030 @default.
- W2123144950 cites W1987184389 @default.
- W2123144950 cites W1995695956 @default.
- W2123144950 cites W2002210641 @default.
- W2123144950 cites W2002729096 @default.
- W2123144950 cites W2009954812 @default.
- W2123144950 cites W2020087630 @default.
- W2123144950 cites W2027787897 @default.
- W2123144950 cites W2037822482 @default.
- W2123144950 cites W2056134842 @default.
- W2123144950 cites W2057894657 @default.
- W2123144950 cites W2059830231 @default.
- W2123144950 cites W2071592895 @default.
- W2123144950 cites W2073303763 @default.
- W2123144950 cites W2078594804 @default.
- W2123144950 cites W2090225411 @default.
- W2123144950 cites W2091926120 @default.
- W2123144950 cites W2098909392 @default.
- W2123144950 cites W2110824674 @default.
- W2123144950 cites W2114145458 @default.
- W2123144950 cites W2118121572 @default.
- W2123144950 cites W2120697535 @default.
- W2123144950 cites W2123319557 @default.
- W2123144950 cites W2127128984 @default.
- W2123144950 cites W2149824864 @default.
- W2123144950 cites W2150045184 @default.
- W2123144950 cites W2150237311 @default.
- W2123144950 cites W2155500251 @default.
- W2123144950 cites W2157547973 @default.
- W2123144950 cites W2163612151 @default.
- W2123144950 cites W2169286706 @default.
- W2123144950 cites W4254164052 @default.
- W2123144950 doi "https://doi.org/10.1093/pcp/pch099" @default.
- W2123144950 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15215502" @default.
- W2123144950 hasPublicationYear "2004" @default.
- W2123144950 type Work @default.
- W2123144950 sameAs 2123144950 @default.
- W2123144950 citedByCount "176" @default.
- W2123144950 countsByYear W21231449502012 @default.
- W2123144950 countsByYear W21231449502013 @default.
- W2123144950 countsByYear W21231449502014 @default.
- W2123144950 countsByYear W21231449502015 @default.
- W2123144950 countsByYear W21231449502016 @default.
- W2123144950 countsByYear W21231449502017 @default.
- W2123144950 countsByYear W21231449502018 @default.
- W2123144950 countsByYear W21231449502019 @default.
- W2123144950 countsByYear W21231449502020 @default.
- W2123144950 countsByYear W21231449502021 @default.
- W2123144950 countsByYear W21231449502022 @default.
- W2123144950 countsByYear W21231449502023 @default.
- W2123144950 crossrefType "journal-article" @default.
- W2123144950 hasAuthorship W2123144950A5006947150 @default.
- W2123144950 hasAuthorship W2123144950A5034791415 @default.
- W2123144950 hasAuthorship W2123144950A5043878996 @default.
- W2123144950 hasAuthorship W2123144950A5056591341 @default.
- W2123144950 hasAuthorship W2123144950A5060352866 @default.
- W2123144950 hasAuthorship W2123144950A5068540369 @default.
- W2123144950 hasAuthorship W2123144950A5068950868 @default.
- W2123144950 hasAuthorship W2123144950A5069711133 @default.
- W2123144950 hasAuthorship W2123144950A5077910250 @default.
- W2123144950 hasAuthorship W2123144950A5082565276 @default.
- W2123144950 hasBestOaLocation W21231449501 @default.
- W2123144950 hasConcept C102568950 @default.
- W2123144950 hasConcept C104317684 @default.
- W2123144950 hasConcept C104397665 @default.
- W2123144950 hasConcept C119062480 @default.
- W2123144950 hasConcept C143065580 @default.
- W2123144950 hasConcept C144647389 @default.
- W2123144950 hasConcept C158617107 @default.
- W2123144950 hasConcept C168240541 @default.
- W2123144950 hasConcept C181199279 @default.
- W2123144950 hasConcept C185592680 @default.
- W2123144950 hasConcept C190062978 @default.
- W2123144950 hasConcept C27239569 @default.
- W2123144950 hasConcept C2775988993 @default.
- W2123144950 hasConcept C2779491563 @default.
- W2123144950 hasConcept C41625074 @default.
- W2123144950 hasConcept C46111723 @default.
- W2123144950 hasConcept C55493867 @default.
- W2123144950 hasConcept C85265743 @default.