FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2123161878> ?p ?o ?g. }

• W2123161878 endingPage "691" @default.
• W2123161878 startingPage "685" @default.
• W2123161878 abstract "Recent studies have demonstrated that transplanted bone marrow-derived stem cells (BMCs) possess a broad differentiation potential and are able to form new cardiomyocytes. However, the identity of BMCs as true cardiomyocytes is still ambiguous. Therefore, we investigated the fate of transplanted fluorescence labeled BMCs and cardiomyocytes in co-culture.For cell tracking we used two different fluorescent probes, Vybrant/DiO and Vybrant/DiI. BMCs were taken from human sternal marrow, purified using a Ficoll-gradient-centrifugation, treated with 5-azacytidine and stained with Vybrant/DiO. Furthermore, isolated spontaneous beating cardiomyocytes of neonatal rats (CM) were labeled with Vybrant/DiI. Thereafter, the BMCs were transplanted into CM-cultures and investigated on day 1, 4, 7, 14 and 28 using two-color fluorescence phenotyping by laser-scanning-cytometry (LSC). Two-color positive cells were harvested by patch-clamp technique and beta-MHC mRNA expression was analyzed by single-cell PCR.Two different morphological phenotypes were observed by LSC. First, isolated DiO labeled BMCs without contact or with direct cell contact to DiI labeled CMs. Second, some BMCs and CMs were double positive for DiO/DiI spontaneously forming hybrids. This population increased by 18% from day 1 to 4 and decreased only slightly until day 28. Additionally, few two-color positive cell formations expressed both human and rat specific beta-MHC mRNA as well as only human beta-MHC mRNA indicating that cell-fusion and transdifferentiation has occurred.These observations provide in vitro evidence for spontaneous cell fusion and transdifferentiation of BMCs in co-culture, raising the possibility that the observed phenomenons may contribute to development or maintenance of these cell types." @default.
• W2123161878 created "2016-06-24" @default.
• W2123161878 creator A5001439184 @default.
• W2123161878 creator A5005800061 @default.
• W2123161878 creator A5009463511 @default.
• W2123161878 creator A5015237090 @default.
• W2123161878 creator A5042925324 @default.
• W2123161878 creator A5058190750 @default.
• W2123161878 creator A5067285590 @default.
• W2123161878 creator A5068024917 @default.
• W2123161878 date "2005-11-01" @default.
• W2123161878 modified "2023-10-18" @default.
• W2123161878 title "Fusion of bone marrow-derived stem cells with cardiomyocytes in a heterologous in vitro model" @default.
• W2123161878 cites W1631250427 @default.
• W2123161878 cites W1979993133 @default.
• W2123161878 cites W1981522023 @default.
• W2123161878 cites W1991248271 @default.
• W2123161878 cites W1991374176 @default.
• W2123161878 cites W1992141131 @default.
• W2123161878 cites W2005319907 @default.
• W2123161878 cites W2016823790 @default.
• W2123161878 cites W2063969136 @default.
• W2123161878 cites W2068380542 @default.
• W2123161878 cites W2090041902 @default.
• W2123161878 cites W2097834438 @default.
• W2123161878 cites W2104898886 @default.
• W2123161878 cites W2109794889 @default.
• W2123161878 cites W2110667326 @default.
• W2123161878 cites W2122059700 @default.
• W2123161878 cites W2129388529 @default.
• W2123161878 cites W2147561450 @default.
• W2123161878 cites W2148220540 @default.
• W2123161878 cites W2150102501 @default.
• W2123161878 cites W2158213998 @default.
• W2123161878 cites W621136948 @default.
• W2123161878 cites W10131357 @default.
• W2123161878 doi "https://doi.org/10.1016/j.ejcts.2005.06.047" @default.
• W2123161878 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16194609" @default.
• W2123161878 hasPublicationYear "2005" @default.
• W2123161878 type Work @default.
• W2123161878 sameAs 2123161878 @default.
• W2123161878 citedByCount "35" @default.
• W2123161878 countsByYear W21231618782012 @default.
• W2123161878 countsByYear W21231618782013 @default.
• W2123161878 countsByYear W21231618782014 @default.
• W2123161878 countsByYear W21231618782015 @default.
• W2123161878 countsByYear W21231618782018 @default.
• W2123161878 crossrefType "journal-article" @default.
• W2123161878 hasAuthorship W2123161878A5001439184 @default.
• W2123161878 hasAuthorship W2123161878A5005800061 @default.
• W2123161878 hasAuthorship W2123161878A5009463511 @default.
• W2123161878 hasAuthorship W2123161878A5015237090 @default.
• W2123161878 hasAuthorship W2123161878A5042925324 @default.
• W2123161878 hasAuthorship W2123161878A5058190750 @default.
• W2123161878 hasAuthorship W2123161878A5067285590 @default.
• W2123161878 hasAuthorship W2123161878A5068024917 @default.
• W2123161878 hasBestOaLocation W21231618781 @default.
• W2123161878 hasConcept C137061746 @default.
• W2123161878 hasConcept C1491633281 @default.
• W2123161878 hasConcept C153911025 @default.
• W2123161878 hasConcept C185592680 @default.
• W2123161878 hasConcept C202751555 @default.
• W2123161878 hasConcept C203014093 @default.
• W2123161878 hasConcept C2778630268 @default.
• W2123161878 hasConcept C2780007613 @default.
• W2123161878 hasConcept C28328180 @default.
• W2123161878 hasConcept C5362183 @default.
• W2123161878 hasConcept C54355233 @default.
• W2123161878 hasConcept C553184892 @default.
• W2123161878 hasConcept C60870556 @default.
• W2123161878 hasConcept C86803240 @default.
• W2123161878 hasConcept C92488253 @default.
• W2123161878 hasConcept C95444343 @default.
• W2123161878 hasConceptScore W2123161878C137061746 @default.
• W2123161878 hasConceptScore W2123161878C1491633281 @default.
• W2123161878 hasConceptScore W2123161878C153911025 @default.
• W2123161878 hasConceptScore W2123161878C185592680 @default.
• W2123161878 hasConceptScore W2123161878C202751555 @default.
• W2123161878 hasConceptScore W2123161878C203014093 @default.
• W2123161878 hasConceptScore W2123161878C2778630268 @default.
• W2123161878 hasConceptScore W2123161878C2780007613 @default.
• W2123161878 hasConceptScore W2123161878C28328180 @default.
• W2123161878 hasConceptScore W2123161878C5362183 @default.
• W2123161878 hasConceptScore W2123161878C54355233 @default.
• W2123161878 hasConceptScore W2123161878C553184892 @default.
• W2123161878 hasConceptScore W2123161878C60870556 @default.
• W2123161878 hasConceptScore W2123161878C86803240 @default.
• W2123161878 hasConceptScore W2123161878C92488253 @default.
• W2123161878 hasConceptScore W2123161878C95444343 @default.
• W2123161878 hasIssue "5" @default.
• W2123161878 hasLocation W21231618781 @default.
• W2123161878 hasLocation W21231618782 @default.
• W2123161878 hasOpenAccess W2123161878 @default.
• W2123161878 hasPrimaryLocation W21231618781 @default.
• W2123161878 hasRelatedWork W159911074 @default.
• W2123161878 hasRelatedWork W1968107606 @default.
• W2123161878 hasRelatedWork W1971266684 @default.
• W2123161878 hasRelatedWork W2016823790 @default.

• next