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• W2123188025 endingPage "53" @default.
• W2123188025 startingPage "45" @default.
• W2123188025 abstract "Adeno-associated virus (AAV) vectors are a platform of choice for in vivo gene transfer applications. However, neutralizing antibodies (NAb) to AAV can be found in humans and some animal species as a result of exposure to the wild-type virus, and high-titer NAb develop following AAV vector administration. In some conditions, anti-AAV NAb can block transduction with AAV vectors even when present at low titers, thus requiring prescreening before vector administration. Here we describe an improved in vitro, cell-based assay for the determination of NAb titer in serum or plasma samples. The assay is easy to setup and sensitive and, depending on the purpose, can be validated to support clinical development of gene therapy products based on AAV vectors." @default.
• W2123188025 created "2016-06-24" @default.
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• W2123188025 creator A5054401285 @default.
• W2123188025 creator A5080778019 @default.
• W2123188025 date "2015-04-01" @default.
• W2123188025 modified "2023-09-26" @default.
• W2123188025 title "Determination of Anti-Adeno-Associated Virus Vector Neutralizing Antibody Titer with an <i>In Vitro</i> Reporter System" @default.
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• W2123188025 doi "https://doi.org/10.1089/hgtb.2015.037" @default.
• W2123188025 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4403012" @default.
• W2123188025 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25819687" @default.
• W2123188025 hasPublicationYear "2015" @default.
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