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- W2123200328 abstract "SYNOPSIS The localization and transformation of the immobilization antigens of Paramecium aurelia were studied with the use of ferritin‐conjugated antibody. The reaction was extremely specific, ferritin granules being found only on the pellicle and cilia of homologous animals. In the transformation from one antigenic type to another, ferritin granules, conjugated with antibody specific for the “new” antigen, appeared initially on the pellicle and subsequently on the cilia. An electron‐dense counterstain, of potassium permanganate and uranyl acetate, not only increased the detail of the structure of the organism, but also stained the absorbed globulin of the specific antibody as a thick fuzz on pellicle and cilia. This fuzz, unlike the ferritin granules, was clearly visible at very low magnifications of the electron microscope. This gave a quick scanning method, especially useful in transformation experiments, when various stages could be quickly scanned for the first appearance of globulin fuzz." @default.
- W2123200328 created "2016-06-24" @default.
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- W2123200328 date "1963-03-01" @default.
- W2123200328 modified "2023-09-23" @default.
- W2123200328 title "CYTOCHEMICAL LOCALIZATION OF ANTIGENS OF PARAMECIUM BY FERRITIN‐CONJUGATED ANTIBODY AND BY COUNTERSTAINING THE RESULTANT ABSORBED GLOBULIN" @default.
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- W2123200328 doi "https://doi.org/10.1111/j.1365-2818.1963.tb02085.x" @default.
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