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- W2123201278 abstract "Summary The past decade has seen a tremendous increase in RNA research, which has demonstrated that RNAs are involved in many more processes than were previously thought. The dynamics of RNA synthesis towards their regulated activity requires the interplay of RNAs with numerous RNA binding proteins (RBPs). The localization of RNA, a mechanism for controlling translation in a spatial and temporal fashion, requires processing and assembly of RNA into transport granules in the nucleus, transport towards cytoplasmic destinations and regulation of its activity. Compared with animal model systems little is known about RNA dynamics and motility in plants. Commonly used methods to study RNA transport and localization are time‐consuming, and require expensive equipment and a high level of experimental skill. Here, we introduce the λN 22 RNA stem‐loop binding system for the in vivo visualization of RNA in plant cells. The λN 22 system consists of two components: the λN 22 RNA binding peptide and the corresponding box‐B stem loops. We generated fusions of λN 22 to different fluorophores and a GATEWAY vector series for the simple fusion of any target RNA 5′ or 3′ to box‐B stem loops. We show that the λN 22 system can be used to detect RNAs in transient expression assays, and that it offers advantages compared with the previously described MS2 system. Furthermore, the λN 22 system can be used in combination with the MS2 system to visualize different RNAs simultaneously in the same cell. The toolbox of vectors generated for both systems is easy to use and promises significant progress in our understanding of RNA transport and localization in plant cells." @default.
- W2123201278 created "2016-06-24" @default.
- W2123201278 creator A5013870582 @default.
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- W2123201278 date "2012-04-13" @default.
- W2123201278 modified "2023-10-16" @default.
- W2123201278 title "In vivo visualization of RNA in plants cells using the λN22 system and a GATEWAY-compatible vector series for candidate RNAs" @default.
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- W2123201278 doi "https://doi.org/10.1111/j.1365-313x.2012.04923.x" @default.
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