FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2123217337> ?p ?o ?g. }

• W2123217337 endingPage "941" @default.
• W2123217337 startingPage "933" @default.
• W2123217337 abstract "Elastase is a major virulence factor in Pseudomonas aeruginosa that is believed to cause extensive tissue damage during infection in the human host. Elastase is secreted in non-mucoid P. aeruginosa. It is known that secretion of most virulence factors such as elastase, lipase, exotoxin A, etc., in P. aeruginosa is greatly reduced in alginate-secreting mucoid cells isolated from the lungs of cystic fibrosis (CF) patients. We have previously reported that in mucoid P. aeruginosaan intracellular protease cleaves the 16 kDa form of nucleoside diphosphate kinase (Ndk) to a truncated 12 kDa form. This smaller form is membrane associated and has been observed to form complexes with specific proteins to predominantly generate GTP, an important molecule in alginate synthesis. The main aim of this study was to purify and characterize this protease. The protease was purified by hydrophobic interaction chromatography of the crude extract of mucoid P. aeruginosa 8821, a CF isolate. Further analysis using a gelatin containing SDS–polyacrylamide gel detected the presence of a 103 kDa protease, which when boiled, migrated as a 33 kDa protein on a SDS–polyacrylamide gel. The first 10 amino acids from the N-terminus of the 33 kDa protease showed 100% identity to the mature form of elastase. An elastase-negative lasB ::Cm knock-out mutant in the mucoid 8821 background was constructed, and it showed a non-mucoid phenotype. This mutant showed the presence of only the 16 kDa form of Ndk both in the cytoplasm and membrane fractions. We present evidence for the retention of active elastase in the periplasm of mucoid P. aeruginosa and its role in the generation of the 12 kDa form of Ndk. Finally, we demonstrate that elastase, when overproduced in both mucoid and non-mucoid cells, stimulates alginate synthesis. This suggests that the genetic rearrangements that trigger mucoidy in P. aeruginosa also allow retention of elastase in the periplasm in an active oligomeric form that facilitates cleavage of 16 kDa Ndk to its 12 kDa form for the generation of GTP, required for alginate synthesis." @default.
• W2123217337 created "2016-06-24" @default.
• W2123217337 creator A5008527036 @default.
• W2123217337 creator A5008952399 @default.
• W2123217337 creator A5044903709 @default.
• W2123217337 date "1998-12-01" @default.
• W2123217337 modified "2023-10-18" @default.
• W2123217337 title "Cellular function of elastase in Pseudomonas aeruginosa: role in the cleavage of nucleoside diphosphate kinase and in alginate synthesis" @default.
• W2123217337 cites W1484938390 @default.
• W2123217337 cites W1501323995 @default.
• W2123217337 cites W1641710824 @default.
• W2123217337 cites W1813709322 @default.
• W2123217337 cites W1874411209 @default.
• W2123217337 cites W1889610427 @default.
• W2123217337 cites W1911515883 @default.
• W2123217337 cites W1918146145 @default.
• W2123217337 cites W1948277616 @default.
• W2123217337 cites W1948956821 @default.
• W2123217337 cites W1965574262 @default.
• W2123217337 cites W1977105094 @default.
• W2123217337 cites W1994116618 @default.
• W2123217337 cites W1996527079 @default.
• W2123217337 cites W2005391739 @default.
• W2123217337 cites W2014417670 @default.
• W2123217337 cites W2034994151 @default.
• W2123217337 cites W2039071520 @default.
• W2123217337 cites W2040107168 @default.
• W2123217337 cites W2053454626 @default.
• W2123217337 cites W2053764628 @default.
• W2123217337 cites W2100837269 @default.
• W2123217337 cites W2102321085 @default.
• W2123217337 cites W2103615189 @default.
• W2123217337 cites W2109826394 @default.
• W2123217337 cites W2110573124 @default.
• W2123217337 cites W2115368037 @default.
• W2123217337 cites W2118260061 @default.
• W2123217337 cites W2120744835 @default.
• W2123217337 cites W2125867320 @default.
• W2123217337 cites W2126378019 @default.
• W2123217337 cites W2128917908 @default.
• W2123217337 cites W2131442384 @default.
• W2123217337 cites W2132417094 @default.
• W2123217337 cites W2146881880 @default.
• W2123217337 cites W2152430830 @default.
• W2123217337 cites W2152450950 @default.
• W2123217337 cites W2158937196 @default.
• W2123217337 cites W2161344845 @default.
• W2123217337 cites W2161951965 @default.
• W2123217337 cites W2169545551 @default.
• W2123217337 cites W4255227858 @default.
• W2123217337 doi "https://doi.org/10.1046/j.1365-2958.1998.01121.x" @default.
• W2123217337 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9988471" @default.
• W2123217337 hasPublicationYear "1998" @default.
• W2123217337 type Work @default.
• W2123217337 sameAs 2123217337 @default.
• W2123217337 citedByCount "95" @default.
• W2123217337 countsByYear W21232173372012 @default.
• W2123217337 countsByYear W21232173372013 @default.
• W2123217337 countsByYear W21232173372014 @default.
• W2123217337 countsByYear W21232173372016 @default.
• W2123217337 countsByYear W21232173372017 @default.
• W2123217337 countsByYear W21232173372018 @default.
• W2123217337 countsByYear W21232173372019 @default.
• W2123217337 countsByYear W21232173372020 @default.
• W2123217337 countsByYear W21232173372021 @default.
• W2123217337 countsByYear W21232173372022 @default.
• W2123217337 countsByYear W21232173372023 @default.
• W2123217337 crossrefType "journal-article" @default.
• W2123217337 hasAuthorship W2123217337A5008527036 @default.
• W2123217337 hasAuthorship W2123217337A5008952399 @default.
• W2123217337 hasAuthorship W2123217337A5044903709 @default.
• W2123217337 hasBestOaLocation W21232173371 @default.
• W2123217337 hasConcept C101660505 @default.
• W2123217337 hasConcept C104317684 @default.
• W2123217337 hasConcept C153911025 @default.
• W2123217337 hasConcept C181199279 @default.
• W2123217337 hasConcept C182179738 @default.
• W2123217337 hasConcept C2776714187 @default.
• W2123217337 hasConcept C2777637488 @default.
• W2123217337 hasConcept C2777807008 @default.
• W2123217337 hasConcept C2781044401 @default.
• W2123217337 hasConcept C2781307694 @default.
• W2123217337 hasConcept C40767141 @default.
• W2123217337 hasConcept C523546767 @default.
• W2123217337 hasConcept C54355233 @default.
• W2123217337 hasConcept C55493867 @default.
• W2123217337 hasConcept C55728118 @default.
• W2123217337 hasConcept C86803240 @default.
• W2123217337 hasConcept C89423630 @default.
• W2123217337 hasConceptScore W2123217337C101660505 @default.
• W2123217337 hasConceptScore W2123217337C104317684 @default.
• W2123217337 hasConceptScore W2123217337C153911025 @default.
• W2123217337 hasConceptScore W2123217337C181199279 @default.
• W2123217337 hasConceptScore W2123217337C182179738 @default.
• W2123217337 hasConceptScore W2123217337C2776714187 @default.
• W2123217337 hasConceptScore W2123217337C2777637488 @default.
• W2123217337 hasConceptScore W2123217337C2777807008 @default.
• W2123217337 hasConceptScore W2123217337C2781044401 @default.

• next