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• W2123235908 startingPage "317" @default.
• W2123235908 abstract "ABSTRACT Activation of the epidermal growth factor receptor (EGFR) by EGF results in binding of clathrin adaptor protein complex AP-2 to the receptor cytoplasmic tail. The transient interaction with AP-2 is thought to be responsible for the selective recruitment of the EGFR into coated pits during endocytosis. In this study we found that EGF-induced EGFR/AP-2 association, measured by co-immunoprecipitation, persists after receptor internalization. Double-label immunofluorescence of EGF-treated A-431 and COS-1 cells revealed the presence of AP-2, clathrin and eps15, another component of the plasma membrane coated pits, in the large perinuclear endosomes loaded with EGFRs. By optical sectioning and image deconvolution, the immunoreactivities were seen to be distributed within vesicular and tubular elements of these endosomes. In addition, these compartments contained the transferrin receptors and a EEA.1 protein, markers of early endosomes. Furthermore, Golgi clathrin adaptor complex AP-1 was found in EGFR-containing endosomes and EGFR immunoprecipitates in A-431 cells. The direct interaction of the EGFR with µ1 as well as µ2 subunits of AP-1 and AP-2, correspondingly, was shown using the yeast two-hybrid assay. Brefeldin A, a drug that releases AP-1 from the trans-Golgi membranes, had no effect on AP-1 association with endosomes and its co-precipitation with EGFR. Taken together, the data suggest that endosomal EGFR-AP complexes make up a significant portion of the total amount of these complexes detectable by coimmunoprecipitation. It can be proposed that APs are capable of binding to the endosomal membrane via a mechanism that requires AP interaction with the intracellular tails of multimeric receptors like activated EGFR, which in turn allows recruitment of clathrin and eps15. The hypothesis that the competition between adaptor complexes for binding to the receptor tails in endosomes may regulate of the sorting of receptors is discussed." @default.
• W2123235908 created "2016-06-24" @default.
• W2123235908 creator A5022727157 @default.
• W2123235908 creator A5033899179 @default.
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• W2123235908 date "1999-02-01" @default.
• W2123235908 modified "2023-09-24" @default.
• W2123235908 title "Clathrin, adaptors and eps15 in endosomes containing activated epidermal growth factor receptors" @default.
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• W2123235908 doi "https://doi.org/10.1242/jcs.112.3.317" @default.
• W2123235908 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9885285" @default.
• W2123235908 hasPublicationYear "1999" @default.
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