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- W2123459894 abstract "Trypanosomes are the causative agents of many diseases of medical and veterinary importance, including sleeping sickness and nagana in Africa, and Chagas disease in South America. Accurate identification of trypanosome species is essential, as some species are morphologically indistinguishable, yet differ greatly in their pathogenicity. A range of molecular tools has been developed for identification of species and strains of trypanosomes. PCR, using primer sets designed to amplify a specific DNA fragment from each trypanosome species, is frequently used. More recently, generic systems have been developed that can potentially recognize all trypanosome species, such as amplification of the internal transcribed spacer and fluorescent fragment length barcoding, both of which use interspecies size variation in PCR fragments amplified from the ribosomal RNA locus. Loop-mediated isothermal amplification is a promising technique and is able to detect trypanosomes in blood, serum and cerebrospinal fluid. The advantages of these techniques for high-throughput and sensitive molecular identification will be discussed." @default.
- W2123459894 created "2016-06-24" @default.
- W2123459894 creator A5014702689 @default.
- W2123459894 creator A5040170526 @default.
- W2123459894 date "2008-04-01" @default.
- W2123459894 modified "2023-09-27" @default.
- W2123459894 title "New molecular tools for the identification of trypanosome species" @default.
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- W2123459894 doi "https://doi.org/10.2217/17460913.3.2.167" @default.
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