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• W2123483096 abstract "• A real-time PCR protocol based on 18S rDNA sequences was developed to provide a specific, sensitive and quantitative assay for the root-infecting virus vector Polymyxa graminis. • The assay was calibrated with zoospore suspensions and inoculated roots and then shown to work with naturally infected plant roots and infested soil. Both the temperate P. graminis ribotypes previously described are detected but are not distinguished. DNA from related plasmodiophorids and from a range of fungi and plants was not detected. • Different genotypes of Triticum were grown in a soil infested with P. graminis and Soil-borne cereal mosaic virus (SBCMV). The genotypes differed in susceptibility to P. graminis, the least susceptible being the Triticum monococcum accession K-58505. • Conventional PCR assays and sequencing of amplified rDNA fragments showed that P. graminis isolates infecting wheat were mostly, but not exclusively, of ribotype II. Ribotype II was clearly associated with SBCMV transmission and seems to occur preferentially on wheat whereas ribotype I is mostly associated with barley." @default.
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• W2123483096 date "2004-12-20" @default.
• W2123483096 modified "2023-10-16" @default.
• W2123483096 title "The use of conventional and quantitative real‐time PCR assays for <i>Polymyxa graminis</i> to examine host plant resistance, inoculum levels and intraspecific variation" @default.
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• W2123483096 doi "https://doi.org/10.1111/j.1469-8137.2004.01291.x" @default.
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