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• W2123485755 abstract "RATIONALE Carbohydrates are highly variable in structure owing to differences in their anomeric configurations, monomer stereochemistry, inter‐residue linkage positions and general branching features. The separation of carbohydrate isomers poses a great challenge for current analytical techniques. METHODS The isomeric heterogeneity of disaccharide ions and monosaccharide‐glycolaldehyde product ions was evaluated using electrospray traveling wave ion mobility mass spectrometry (Synapt G2 high‐definition mass spectrometer) in both positive and negative ion modes. RESULTS The separation of isomeric disaccharide ions was observed but not fully achieved based on their mobility profiles. The mobilities of isomeric product ions, the monosaccharide‐glycolaldehydes, derived from different disaccharide isomers were measured. Multiple mobility peaks were observed for both monosaccharide‐glycolaldehyde cations and anions, indicating that there was more than one structural configuration in the gas phase as verified by NMR in solution. More importantly, the mobility patterns for isomeric monosaccharide‐glycolaldehyde product ions were different, which enabled partial characterization of their respective disaccharide ions. Abundant disaccharide cluster ions were also observed. The results showed that a majority of isomeric cluster ions had different drift times and, moreover, more than one mobility peak was detected for a number of specific cluster ions. CONCLUSIONS It is demonstrated that ion mobility mass spectrometry is an advantageous method to assess the isomeric heterogeneity of carbohydrate compounds. It is capable of differentiating different types of carbohydrate ions having identical m/z values as well as multiple structural configurations of single compounds. Copyright © 2013 John Wiley & Sons, Ltd." @default.
• W2123485755 created "2016-06-24" @default.
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• W2123485755 date "2013-10-31" @default.
• W2123485755 modified "2023-10-14" @default.
• W2123485755 title "Ion mobility mass spectrometry analysis of isomeric disaccharide precursor, product and cluster ions" @default.
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• W2123485755 doi "https://doi.org/10.1002/rcm.6720" @default.
• W2123485755 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4317727" @default.
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