Matches in SemOpenAlex for { <https://semopenalex.org/work/W2123608704> ?p ?o ?g. }
- W2123608704 endingPage "528" @default.
- W2123608704 startingPage "521" @default.
- W2123608704 abstract "A small heat-shock protein (sHSP) that shows molecular chaperone activity in vitro was recently purified from mature chestnut (Castanea sativa) cotyledons. This protein, renamed here as CsHSP17. 5, belongs to cytosolic class I, as revealed by cDNA sequencing and immunoelectron microscopy. Recombinant CsHSP17.5 was overexpressed in Escherichia coli to study its possible function under stress conditions. Upon transfer from 37 degrees C to 50 degrees C, a temperature known to cause cell autolysis, those cells that accumulated CsHSP17.5 showed improved viability compared with control cultures. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of cell lysates suggested that such a protective effect in vivo is due to the ability of recombinant sHSP to maintain soluble cytosolic proteins in their native conformation, with little substrate specificity. To test the recent hypothesis that sHSPs may be involved in protection against cold stress, we also studied the viability of recombinant cells at 4 degrees C. Unlike the major heat-induced chaperone, GroEL/ES, the chestnut sHSP significantly enhanced cell survivability at this temperature. CsHSP17.5 thus represents an example of a HSP capable of protecting cells against both thermal extremes. Consistent with these findings, high-level induction of homologous transcripts was observed in vegetative tissues of chestnut plantlets exposed to either type of thermal stress but not salt stress." @default.
- W2123608704 created "2016-06-24" @default.
- W2123608704 creator A5013951178 @default.
- W2123608704 creator A5025210422 @default.
- W2123608704 creator A5031514818 @default.
- W2123608704 creator A5034221645 @default.
- W2123608704 creator A5044423877 @default.
- W2123608704 creator A5064526013 @default.
- W2123608704 creator A5077503995 @default.
- W2123608704 creator A5086747276 @default.
- W2123608704 date "1999-06-01" @default.
- W2123608704 modified "2023-10-06" @default.
- W2123608704 title "Heterologous Expression of a Plant Small Heat-Shock Protein Enhances <i>Escherichia coli</i>Viability under Heat and Cold Stress1" @default.
- W2123608704 cites W1537274817 @default.
- W2123608704 cites W1603717990 @default.
- W2123608704 cites W1821805481 @default.
- W2123608704 cites W1834847896 @default.
- W2123608704 cites W1871950358 @default.
- W2123608704 cites W1936320257 @default.
- W2123608704 cites W1976300942 @default.
- W2123608704 cites W1978545193 @default.
- W2123608704 cites W1980948387 @default.
- W2123608704 cites W1998821979 @default.
- W2123608704 cites W2003394416 @default.
- W2123608704 cites W2008938176 @default.
- W2123608704 cites W2010335064 @default.
- W2123608704 cites W2012999417 @default.
- W2123608704 cites W2015585021 @default.
- W2123608704 cites W2022038675 @default.
- W2123608704 cites W2030414352 @default.
- W2123608704 cites W2032625890 @default.
- W2123608704 cites W2039315146 @default.
- W2123608704 cites W2044031095 @default.
- W2123608704 cites W2045866234 @default.
- W2123608704 cites W2054664724 @default.
- W2123608704 cites W2060644195 @default.
- W2123608704 cites W2077614843 @default.
- W2123608704 cites W2080943796 @default.
- W2123608704 cites W2084664870 @default.
- W2123608704 cites W2092686336 @default.
- W2123608704 cites W2099689112 @default.
- W2123608704 cites W2101845512 @default.
- W2123608704 cites W2105472242 @default.
- W2123608704 cites W2112383686 @default.
- W2123608704 cites W2119999468 @default.
- W2123608704 cites W2124399930 @default.
- W2123608704 cites W2127033103 @default.
- W2123608704 cites W2148573587 @default.
- W2123608704 cites W2154836366 @default.
- W2123608704 cites W227264993 @default.
- W2123608704 cites W2462173644 @default.
- W2123608704 cites W4243642973 @default.
- W2123608704 cites W4376043815 @default.
- W2123608704 doi "https://doi.org/10.1104/pp.120.2.521" @default.
- W2123608704 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/59290" @default.
- W2123608704 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10364403" @default.
- W2123608704 hasPublicationYear "1999" @default.
- W2123608704 type Work @default.
- W2123608704 sameAs 2123608704 @default.
- W2123608704 citedByCount "141" @default.
- W2123608704 countsByYear W21236087042012 @default.
- W2123608704 countsByYear W21236087042013 @default.
- W2123608704 countsByYear W21236087042014 @default.
- W2123608704 countsByYear W21236087042015 @default.
- W2123608704 countsByYear W21236087042016 @default.
- W2123608704 countsByYear W21236087042017 @default.
- W2123608704 countsByYear W21236087042018 @default.
- W2123608704 countsByYear W21236087042019 @default.
- W2123608704 countsByYear W21236087042020 @default.
- W2123608704 countsByYear W21236087042021 @default.
- W2123608704 countsByYear W21236087042022 @default.
- W2123608704 countsByYear W21236087042023 @default.
- W2123608704 crossrefType "journal-article" @default.
- W2123608704 hasAuthorship W2123608704A5013951178 @default.
- W2123608704 hasAuthorship W2123608704A5025210422 @default.
- W2123608704 hasAuthorship W2123608704A5031514818 @default.
- W2123608704 hasAuthorship W2123608704A5034221645 @default.
- W2123608704 hasAuthorship W2123608704A5044423877 @default.
- W2123608704 hasAuthorship W2123608704A5064526013 @default.
- W2123608704 hasAuthorship W2123608704A5077503995 @default.
- W2123608704 hasAuthorship W2123608704A5086747276 @default.
- W2123608704 hasBestOaLocation W21236087041 @default.
- W2123608704 hasConcept C104317684 @default.
- W2123608704 hasConcept C104810894 @default.
- W2123608704 hasConcept C108305142 @default.
- W2123608704 hasConcept C116084860 @default.
- W2123608704 hasConcept C142724271 @default.
- W2123608704 hasConcept C153911025 @default.
- W2123608704 hasConcept C159654299 @default.
- W2123608704 hasConcept C181199279 @default.
- W2123608704 hasConcept C187882448 @default.
- W2123608704 hasConcept C205260736 @default.
- W2123608704 hasConcept C2775962898 @default.
- W2123608704 hasConcept C2777174735 @default.
- W2123608704 hasConcept C2778913249 @default.
- W2123608704 hasConcept C2780115692 @default.
- W2123608704 hasConcept C2993140218 @default.
- W2123608704 hasConcept C40767141 @default.