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• W2123670297 endingPage "6570" @default.
• W2123670297 startingPage "6565" @default.
• W2123670297 abstract "ABSTRACT A deletion mutation in the gene rpoZ of Mycobacterium smegmatis causes reduced growth rate and a change in colony morphology. During purification of RNA polymerase from the mutant strain, the β′ subunit undergoes fragmentation but the fragments remain associated with the enzyme and maintain it in an active state until the whole destabilized assembly breaks down in the final step of purification. Complementation of the mutant strain with an integrated copy of the wild-type rpoZ brings back the wild-type colony morphology and improves the growth rate and activity of the enzyme, and the integrity of the β′ subunit remains unaffected." @default.
• W2123670297 created "2016-06-24" @default.
• W2123670297 creator A5022550195 @default.
• W2123670297 creator A5033077936 @default.
• W2123670297 creator A5047691044 @default.
• W2123670297 date "2005-09-15" @default.
• W2123670297 modified "2023-09-25" @default.
• W2123670297 title "Deletion of the Gene <i>rpoZ</i> , Encoding the ω Subunit of RNA Polymerase, in <i>Mycobacterium smegmatis</i> Results in Fragmentation of the β′ Subunit in the Enzyme Assembly" @default.
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• W2123670297 doi "https://doi.org/10.1128/jb.187.18.6565-6570.2005" @default.
• W2123670297 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1273006" @default.
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