FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2123717662> ?p ?o ?g. }

• W2123717662 endingPage "343" @default.
• W2123717662 startingPage "330" @default.
• W2123717662 abstract "Large-scale pharmacogenetics and complex disease association studies will require typing of thousands of single-nucleotide polymorphisms (SNPs) in thousands of individuals. Such projects would benefit from a genotyping system with accuracy >99% and a failure rate <5% on a simple, reliable, and flexible platform. However, such a system is not yet available for routine laboratory use. We have evaluated a modification of the previously reported Invader SNP-typing chemistry for use in a genotyping laboratory and tested its automation. The Invader technology uses a Flap Endonuclease for allele discrimination and a universal fluorescence resonance energy transfer (FRET) reporter system. Three hundred and eighty-four individuals were genotyped across a panel of 36 SNPs and one insertion/deletion polymorphism with Invader assays using PCR product as template, a total of 14,208 genotypes. An average failure rate of 2.3% was recorded, mostly associated with PCR failure, and the typing was 99.2% accurate when compared with genotypes generated with established techniques. An average signal-to-noise ratio (9:1) was obtained. The high degree of discrimination for single base changes, coupled with homogeneous format, has allowed us to deploy liquid handling robots in a 384-well microtitre plate format and an automated end-point capture of fluorescent signal. Simple semiautomated data interpretation allows the generation of ∼25,000 genotypes per person per week, which is 10-fold greater than gel-based SNP typing and microsatellite typing in our laboratory. Savings on labor costs are considerable. We conclude that Invader chemistry using PCR products as template represents a useful technology for typing large numbers of SNPs rapidly and efficiently." @default.
• W2123717662 created "2016-06-24" @default.
• W2123717662 creator A5001458487 @default.
• W2123717662 creator A5014842174 @default.
• W2123717662 creator A5021814227 @default.
• W2123717662 creator A5022180165 @default.
• W2123717662 creator A5029747003 @default.
• W2123717662 creator A5037745531 @default.
• W2123717662 creator A5044772197 @default.
• W2123717662 creator A5052837634 @default.
• W2123717662 creator A5060502895 @default.
• W2123717662 creator A5065878246 @default.
• W2123717662 creator A5066852526 @default.
• W2123717662 creator A5074438248 @default.
• W2123717662 creator A5081309660 @default.
• W2123717662 creator A5081721372 @default.
• W2123717662 date "2000-03-01" @default.
• W2123717662 modified "2023-10-17" @default.
• W2123717662 title "Evaluation of Single Nucleotide Polymorphism Typing with Invader on PCR Amplicons and Its Automation" @default.
• W2123717662 cites W1538518956 @default.
• W2123717662 cites W1559760142 @default.
• W2123717662 cites W1589411629 @default.
• W2123717662 cites W1630413167 @default.
• W2123717662 cites W1711580613 @default.
• W2123717662 cites W1958523811 @default.
• W2123717662 cites W1978920258 @default.
• W2123717662 cites W1987739795 @default.
• W2123717662 cites W2015602170 @default.
• W2123717662 cites W2020663735 @default.
• W2123717662 cites W2022187295 @default.
• W2123717662 cites W2024196981 @default.
• W2123717662 cites W2028383523 @default.
• W2123717662 cites W2034957838 @default.
• W2123717662 cites W2035804334 @default.
• W2123717662 cites W2042103448 @default.
• W2123717662 cites W2046056761 @default.
• W2123717662 cites W2047948939 @default.
• W2123717662 cites W2053277918 @default.
• W2123717662 cites W2062042643 @default.
• W2123717662 cites W2062253025 @default.
• W2123717662 cites W2065362054 @default.
• W2123717662 cites W2068412525 @default.
• W2123717662 cites W2071692287 @default.
• W2123717662 cites W2073476576 @default.
• W2123717662 cites W2092483013 @default.
• W2123717662 cites W2105025553 @default.
• W2123717662 cites W2127082697 @default.
• W2123717662 cites W2130445686 @default.
• W2123717662 cites W2144345532 @default.
• W2123717662 cites W2160017466 @default.
• W2123717662 cites W2160524963 @default.
• W2123717662 cites W2160782760 @default.
• W2123717662 cites W2161916629 @default.
• W2123717662 cites W4254753097 @default.
• W2123717662 doi "https://doi.org/10.1101/gr.10.3.330" @default.
• W2123717662 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/311429" @default.
• W2123717662 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10720574" @default.
• W2123717662 hasPublicationYear "2000" @default.
• W2123717662 type Work @default.
• W2123717662 sameAs 2123717662 @default.
• W2123717662 citedByCount "198" @default.
• W2123717662 countsByYear W21237176622012 @default.
• W2123717662 countsByYear W21237176622013 @default.
• W2123717662 countsByYear W21237176622014 @default.
• W2123717662 countsByYear W21237176622015 @default.
• W2123717662 countsByYear W21237176622016 @default.
• W2123717662 countsByYear W21237176622017 @default.
• W2123717662 countsByYear W21237176622018 @default.
• W2123717662 countsByYear W21237176622019 @default.
• W2123717662 countsByYear W21237176622022 @default.
• W2123717662 crossrefType "journal-article" @default.
• W2123717662 hasAuthorship W2123717662A5001458487 @default.
• W2123717662 hasAuthorship W2123717662A5014842174 @default.
• W2123717662 hasAuthorship W2123717662A5021814227 @default.
• W2123717662 hasAuthorship W2123717662A5022180165 @default.
• W2123717662 hasAuthorship W2123717662A5029747003 @default.
• W2123717662 hasAuthorship W2123717662A5037745531 @default.
• W2123717662 hasAuthorship W2123717662A5044772197 @default.
• W2123717662 hasAuthorship W2123717662A5052837634 @default.
• W2123717662 hasAuthorship W2123717662A5060502895 @default.
• W2123717662 hasAuthorship W2123717662A5065878246 @default.
• W2123717662 hasAuthorship W2123717662A5066852526 @default.
• W2123717662 hasAuthorship W2123717662A5074438248 @default.
• W2123717662 hasAuthorship W2123717662A5081309660 @default.
• W2123717662 hasAuthorship W2123717662A5081721372 @default.
• W2123717662 hasBestOaLocation W21237176621 @default.
• W2123717662 hasConcept C104317684 @default.
• W2123717662 hasConcept C135763542 @default.
• W2123717662 hasConcept C139275648 @default.
• W2123717662 hasConcept C153209595 @default.
• W2123717662 hasConcept C163691529 @default.
• W2123717662 hasConcept C180754005 @default.
• W2123717662 hasConcept C2781209916 @default.
• W2123717662 hasConcept C31467283 @default.
• W2123717662 hasConcept C49105822 @default.
• W2123717662 hasConcept C54355233 @default.
• W2123717662 hasConcept C61320498 @default.
• W2123717662 hasConcept C70721500 @default.

• next