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- W2123861038 abstract "A leukotoxin purified from Enterobacter cloacae culture by saline precipitation, gel chromatography and HPLC was studied as a modulator of reactive oxidant species (ROS) produced by human neutrophils. Chemiluminescence showed that stimulation of ROS was achieved at a low leukotoxin concentration, but ROS production decreased when the toxin was applied at concentrations above 30 microg/mL. Also, the addition of 100 microg toxin/mL significantly reduced the activating effect of phorbol myristate acetate (PMA) and low doses of toxin did not produce an opposite effect toward the stimulation produced by PMA. Normal neutrophils showed a linear correlation between the inverse of ROS production and time, but the kinetic reaction changed when toxins were added to the cells and the ROS formation increased directly with time." @default.
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- W2123861038 date "2001-01-01" @default.
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- W2123861038 title "Enterobacter cloacae leukotoxin: modulation of reactive oxidant species generated by neutrophils" @default.
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- W2123861038 doi "https://doi.org/10.1002/bio.604" @default.
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