FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2123967742> ?p ?o ?g. }

• W2123967742 endingPage "332" @default.
• W2123967742 startingPage "321" @default.
• W2123967742 abstract "Although putative horse embryonic stem (ES)-like cell lines have been obtained recently from in vivo-derived embryos, it is currently not known whether it is possible to obtain ES cell (ESC) lines from somatic cell nuclear transfer (SCNT) and parthenogenetic (PA) embryos. Our aim is to establish culture conditions for the derivation of autologous ESC lines for cell therapy studies in an equine model. Our results indicate that both the use of early-stage blastocysts with a clearly visible inner cell mass (ICM) and the use of pronase to dissect the ICM allow the derivation of a higher proportion of primary ICM outgrowths from PA and SCNT embryos. Primary ICM outgrowths express the molecular markers of pluripotency POU class 5 homeobox 1 (POU5F1) and (sex determining region-Y)-box2 (SOX2), and in some cases, NANOG. Cells obtained after the passages of PA primary ICM outgrowths display alkaline phosphatase (AP) activity and POU5F1, SOX2, caudal-related homeobox-2 (CDX2) and eomesodermin (EOMES) expression, but may lose NANOG. Cystic embryoid body-like structures expressing POU5F1, CDX2 and EOMES were produced from these cells. Immunohistochemical analysis of equine embryos reveals the presence of POU5F1 in trophectoderm, primitive endoderm and ICM. These results suggest that cells obtained after passages of primary ICM outgrowths are positive for trophoblast stem cell markers while expressing POU5F1 and displaying AP activity. Therefore, these cells most likely represent trophoblast cells rather than true ESCs. This study represents an important first step towards the production of autologous equine ESCs for pre-clinical cell therapy studies on large animal models." @default.
• W2123967742 created "2016-06-24" @default.
• W2123967742 creator A5014467839 @default.
• W2123967742 creator A5014635120 @default.
• W2123967742 creator A5023867818 @default.
• W2123967742 creator A5036039589 @default.
• W2123967742 creator A5065494091 @default.
• W2123967742 creator A5074833069 @default.
• W2123967742 creator A5016037693 @default.
• W2123967742 date "2011-03-01" @default.
• W2123967742 modified "2023-09-26" @default.
• W2123967742 title "Trophoblast stem cell marker gene expression in inner cell mass-derived cells from parthenogenetic equine embryos" @default.
• W2123967742 cites W1582404500 @default.
• W2123967742 cites W1887077905 @default.
• W2123967742 cites W1966915633 @default.
• W2123967742 cites W1967510325 @default.
• W2123967742 cites W1980417131 @default.
• W2123967742 cites W1982352752 @default.
• W2123967742 cites W1983627503 @default.
• W2123967742 cites W1994831934 @default.
• W2123967742 cites W2005432188 @default.
• W2123967742 cites W2014165073 @default.
• W2123967742 cites W2014178377 @default.
• W2123967742 cites W2020012816 @default.
• W2123967742 cites W2023507189 @default.
• W2123967742 cites W2028492278 @default.
• W2123967742 cites W2031280968 @default.
• W2123967742 cites W2033604950 @default.
• W2123967742 cites W2033728888 @default.
• W2123967742 cites W2036013289 @default.
• W2123967742 cites W2045771535 @default.
• W2123967742 cites W2048477506 @default.
• W2123967742 cites W2048554758 @default.
• W2123967742 cites W2048833550 @default.
• W2123967742 cites W2060132470 @default.
• W2123967742 cites W2061184843 @default.
• W2123967742 cites W2065353453 @default.
• W2123967742 cites W2072872182 @default.
• W2123967742 cites W2078178837 @default.
• W2123967742 cites W2086943348 @default.
• W2123967742 cites W2096017463 @default.
• W2123967742 cites W2097595052 @default.
• W2123967742 cites W2108931314 @default.
• W2123967742 cites W2110737932 @default.
• W2123967742 cites W2113693767 @default.
• W2123967742 cites W2114647473 @default.
• W2123967742 cites W2122214027 @default.
• W2123967742 cites W2126536572 @default.
• W2123967742 cites W2127100374 @default.
• W2123967742 cites W2140338297 @default.
• W2123967742 cites W2142001424 @default.
• W2123967742 cites W2166002518 @default.
• W2123967742 cites W2171471011 @default.
• W2123967742 cites W3044385279 @default.
• W2123967742 cites W4246254021 @default.
• W2123967742 cites W4253326570 @default.
• W2123967742 doi "https://doi.org/10.1530/rep-09-0536" @default.
• W2123967742 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21209071" @default.
• W2123967742 hasPublicationYear "2011" @default.
• W2123967742 type Work @default.
• W2123967742 sameAs 2123967742 @default.
• W2123967742 citedByCount "24" @default.
• W2123967742 countsByYear W21239677422013 @default.
• W2123967742 countsByYear W21239677422014 @default.
• W2123967742 countsByYear W21239677422015 @default.
• W2123967742 countsByYear W21239677422016 @default.
• W2123967742 countsByYear W21239677422017 @default.
• W2123967742 countsByYear W21239677422018 @default.
• W2123967742 countsByYear W21239677422019 @default.
• W2123967742 countsByYear W21239677422020 @default.
• W2123967742 countsByYear W21239677422021 @default.
• W2123967742 countsByYear W21239677422022 @default.
• W2123967742 countsByYear W21239677422023 @default.
• W2123967742 crossrefType "journal-article" @default.
• W2123967742 hasAuthorship W2123967742A5014467839 @default.
• W2123967742 hasAuthorship W2123967742A5014635120 @default.
• W2123967742 hasAuthorship W2123967742A5016037693 @default.
• W2123967742 hasAuthorship W2123967742A5023867818 @default.
• W2123967742 hasAuthorship W2123967742A5036039589 @default.
• W2123967742 hasAuthorship W2123967742A5065494091 @default.
• W2123967742 hasAuthorship W2123967742A5074833069 @default.
• W2123967742 hasBestOaLocation W21239677421 @default.
• W2123967742 hasConcept C104317684 @default.
• W2123967742 hasConcept C107459253 @default.
• W2123967742 hasConcept C121587040 @default.
• W2123967742 hasConcept C131364181 @default.
• W2123967742 hasConcept C145103041 @default.
• W2123967742 hasConcept C159399396 @default.
• W2123967742 hasConcept C16685009 @default.
• W2123967742 hasConcept C172680121 @default.
• W2123967742 hasConcept C196843134 @default.
• W2123967742 hasConcept C202438428 @default.
• W2123967742 hasConcept C207168726 @default.
• W2123967742 hasConcept C207701140 @default.
• W2123967742 hasConcept C2776953305 @default.
• W2123967742 hasConcept C2777495146 @default.
• W2123967742 hasConcept C2777933648 @default.
• W2123967742 hasConcept C2778177303 @default.

• next