FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W212401> ?p ?o ?g. }

• W212401 endingPage "56" @default.
• W212401 startingPage "547" @default.
• W212401 abstract "Athymic PVG-rnu/rnu rats receiving a single intravenous injection of syngeneic euthymic thoracic duct lymphocytes (TDL) develop normal levels of CD4+ T lymphocytes and survive for more than 2 years in a conventional animal house. We investigated the origin of the T cells (and B cells) in reconstituted nude recipients by transferring TDL carrying either the 3T chromosome marker or the RT6b + Igk-1b allotype or the RT7b (leucocyte-common) allotype markers. Karyotype analysis of spleen and lymph node (LN) cells from 1- to 2-year-old PVG-3T/3T-reconstituted nude recipients, stimulated in vitro with phytohaemagglutinin (PHA), unexpectedly revealed that a majority (79-97%) of dividing cells were of nude origin. However, extensive nude cell division was also recorded in PHA-stimulated cultures using mixtures of euthymic (PVG-3T/3T) and unreconstituted nude spleen cells; the assumption that only T cells divide in PHA-stimulated cultures thus appears to be erroneous. In contrast to the karyotype analysis, sIg- RT6b+ LN cells obtained from nude recipients reconstituted 2 years earlier with PVG-RT6b allotype-marked TDL, were all of donor origin with no indication of a nude-derived sIg- RT6a+ population. Igk-1b+ donor B cells were not found in these same recipients. Dual fluorescence analysis of TDL from 18- to 20-month RT7b-reconstituted nudes showed that 91-100% of CD4+ cells were donor-derived. When tested functionally, sIg- RT7b+ (donor) cells, but not sIg- RT7b- (nude-derived) cells, were able to reject skin allografts and induce local graft-versus-host (GVH) responses. Donor T cells, in contrast to CD4+ cells of nude origin, divided extensively in nude recipients; FACS-purified RT7b+ (donor) TDL retransferred from 17-month primary reconstituted nude rats, expanded further (60-100-fold) in secondary nude recipients. In conclusion, only the donor-derived CD4+ cells in reconstituted nude rats displayed T-cell function; evidence to the contrary from karyotype analysis was flawed. At no stage in their life did uninjected or T-cell reconstituted nude rats develop endogenous cells that in any way resembled CD4+ products of the thymus." @default.
• W212401 created "2016-06-24" @default.
• W212401 creator A5032055781 @default.
• W212401 creator A5033250399 @default.
• W212401 creator A5040545059 @default.
• W212401 creator A5042808179 @default.
• W212401 date "1989-12-01" @default.
• W212401 modified "2023-10-18" @default.
• W212401 title "The origin of T cells in permanently reconstituted old athymic nude rats. Analysis using chromosome or allotype markers." @default.
• W212401 cites W1525360339 @default.
• W212401 cites W1539817818 @default.
• W212401 cites W1540232723 @default.
• W212401 cites W1569095844 @default.
• W212401 cites W16634968 @default.
• W212401 cites W1675660902 @default.
• W212401 cites W1767943166 @default.
• W212401 cites W1928402537 @default.
• W212401 cites W1968637585 @default.
• W212401 cites W1968799745 @default.
• W212401 cites W1982440683 @default.
• W212401 cites W1985215100 @default.
• W212401 cites W1987709478 @default.
• W212401 cites W2008809605 @default.
• W212401 cites W2012337198 @default.
• W212401 cites W2031876252 @default.
• W212401 cites W2032849822 @default.
• W212401 cites W2037707688 @default.
• W212401 cites W2056769875 @default.
• W212401 cites W2070502835 @default.
• W212401 cites W2072804681 @default.
• W212401 cites W2072958605 @default.
• W212401 cites W2078365253 @default.
• W212401 cites W2079428489 @default.
• W212401 cites W2089921240 @default.
• W212401 cites W2118429881 @default.
• W212401 cites W2118807250 @default.
• W212401 cites W2139382219 @default.
• W212401 cites W2161901115 @default.
• W212401 cites W2164411482 @default.
• W212401 cites W2169950577 @default.
• W212401 cites W2240214327 @default.
• W212401 cites W2398621014 @default.
• W212401 cites W64647244 @default.
• W212401 cites W82958451 @default.
• W212401 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1385545" @default.
• W212401 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2575076" @default.
• W212401 hasPublicationYear "1989" @default.
• W212401 type Work @default.
• W212401 sameAs 212401 @default.
• W212401 citedByCount "8" @default.
• W212401 crossrefType "journal-article" @default.
• W212401 hasAuthorship W212401A5032055781 @default.
• W212401 hasAuthorship W212401A5033250399 @default.
• W212401 hasAuthorship W212401A5040545059 @default.
• W212401 hasAuthorship W212401A5042808179 @default.
• W212401 hasConcept C104317684 @default.
• W212401 hasConcept C142724271 @default.
• W212401 hasConcept C147483822 @default.
• W212401 hasConcept C153911025 @default.
• W212401 hasConcept C203014093 @default.
• W212401 hasConcept C2777761686 @default.
• W212401 hasConcept C2780050583 @default.
• W212401 hasConcept C2780278673 @default.
• W212401 hasConcept C2780849966 @default.
• W212401 hasConcept C2780889450 @default.
• W212401 hasConcept C2780931953 @default.
• W212401 hasConcept C2908647359 @default.
• W212401 hasConcept C30278631 @default.
• W212401 hasConcept C30481170 @default.
• W212401 hasConcept C53226629 @default.
• W212401 hasConcept C54355233 @default.
• W212401 hasConcept C71924100 @default.
• W212401 hasConcept C81885089 @default.
• W212401 hasConcept C86803240 @default.
• W212401 hasConcept C99454951 @default.
• W212401 hasConceptScore W212401C104317684 @default.
• W212401 hasConceptScore W212401C142724271 @default.
• W212401 hasConceptScore W212401C147483822 @default.
• W212401 hasConceptScore W212401C153911025 @default.
• W212401 hasConceptScore W212401C203014093 @default.
• W212401 hasConceptScore W212401C2777761686 @default.
• W212401 hasConceptScore W212401C2780050583 @default.
• W212401 hasConceptScore W212401C2780278673 @default.
• W212401 hasConceptScore W212401C2780849966 @default.
• W212401 hasConceptScore W212401C2780889450 @default.
• W212401 hasConceptScore W212401C2780931953 @default.
• W212401 hasConceptScore W212401C2908647359 @default.
• W212401 hasConceptScore W212401C30278631 @default.
• W212401 hasConceptScore W212401C30481170 @default.
• W212401 hasConceptScore W212401C53226629 @default.
• W212401 hasConceptScore W212401C54355233 @default.
• W212401 hasConceptScore W212401C71924100 @default.
• W212401 hasConceptScore W212401C81885089 @default.
• W212401 hasConceptScore W212401C86803240 @default.
• W212401 hasConceptScore W212401C99454951 @default.
• W212401 hasIssue "4" @default.
• W212401 hasLocation W2124011 @default.
• W212401 hasOpenAccess W212401 @default.

• next