Matches in SemOpenAlex for { <https://semopenalex.org/work/W2124061356> ?p ?o ?g. }
- W2124061356 endingPage "4726" @default.
- W2124061356 startingPage "4718" @default.
- W2124061356 abstract "RNA-based duplication mediated by reverse transcriptase (RT), a process termed retrotransposition, is ongoing in humans and is a source of significant inter- and perhaps intraindividual genomic variation. The long interspersed element 1 (LINE-1 or L1) ORF2 protein is the genomic source for RT activity required for mobilization of its own RNA in cis and other RNAs, such as SINE/variable-number tandem-repeat (VNTR)/Alu (SVA) elements, in trans. SVA elements are ∼2-kb hominid-specific noncoding RNAs that have resulted in single-gene disease in humans through insertional mutagenesis or aberrant mRNA splicing. Here, using an SVA retrotransposition cell culture assay in U2OS cells, we investigated SVA domains important in L1-mediated SVA retrotransposition. Partial- and whole-domain deletions revealed that removal of either the Alu-like or SINE-R domain in the context of a full-length SVA has little to no effect, whereas removal of the CT hexamer or the VNTR domain can result in a 75% decrease in activity. Additional experiments demonstrate that the Alu-like fragment alone can retrotranspose at low levels while the addition of the CT hexamer can enhance activity as much as 2-fold compared to that of the full-length SVA. These results suggest that no SVA domain is essential for retrotransposition in U2OS cells and that the 5′ end of SVA (hexamer and Alu-like domain) is sufficient for retrotransposition." @default.
- W2124061356 created "2016-06-24" @default.
- W2124061356 creator A5044261830 @default.
- W2124061356 creator A5062883688 @default.
- W2124061356 creator A5064084333 @default.
- W2124061356 creator A5088244605 @default.
- W2124061356 date "2012-11-01" @default.
- W2124061356 modified "2023-10-12" @default.
- W2124061356 title "The Minimal Active Human SVA Retrotransposon Requires Only the 5′-Hexamer and Alu-Like Domains" @default.
- W2124061356 cites W1528429662 @default.
- W2124061356 cites W1563166241 @default.
- W2124061356 cites W1689369637 @default.
- W2124061356 cites W1944771638 @default.
- W2124061356 cites W1959325762 @default.
- W2124061356 cites W1965141089 @default.
- W2124061356 cites W1969989752 @default.
- W2124061356 cites W1986319478 @default.
- W2124061356 cites W1986929596 @default.
- W2124061356 cites W1988495745 @default.
- W2124061356 cites W1991333931 @default.
- W2124061356 cites W1995827174 @default.
- W2124061356 cites W2007589425 @default.
- W2124061356 cites W2011016615 @default.
- W2124061356 cites W2014439672 @default.
- W2124061356 cites W2015415562 @default.
- W2124061356 cites W2018086201 @default.
- W2124061356 cites W2019469673 @default.
- W2124061356 cites W2019478742 @default.
- W2124061356 cites W2032237595 @default.
- W2124061356 cites W2032679533 @default.
- W2124061356 cites W2035120673 @default.
- W2124061356 cites W2036994672 @default.
- W2124061356 cites W2040741651 @default.
- W2124061356 cites W2040891039 @default.
- W2124061356 cites W2042081010 @default.
- W2124061356 cites W2043603340 @default.
- W2124061356 cites W2046415390 @default.
- W2124061356 cites W2047979193 @default.
- W2124061356 cites W2049997041 @default.
- W2124061356 cites W2059173951 @default.
- W2124061356 cites W2059919630 @default.
- W2124061356 cites W2062250400 @default.
- W2124061356 cites W2064629259 @default.
- W2124061356 cites W2070807615 @default.
- W2124061356 cites W2071501400 @default.
- W2124061356 cites W2075224242 @default.
- W2124061356 cites W2076424822 @default.
- W2124061356 cites W2080056745 @default.
- W2124061356 cites W2084161942 @default.
- W2124061356 cites W2084408009 @default.
- W2124061356 cites W2086739233 @default.
- W2124061356 cites W2091279754 @default.
- W2124061356 cites W2102773855 @default.
- W2124061356 cites W2106021702 @default.
- W2124061356 cites W2109533720 @default.
- W2124061356 cites W2114766453 @default.
- W2124061356 cites W2116338406 @default.
- W2124061356 cites W2119742125 @default.
- W2124061356 cites W2120034162 @default.
- W2124061356 cites W2120041527 @default.
- W2124061356 cites W2120762351 @default.
- W2124061356 cites W2122826014 @default.
- W2124061356 cites W2123883764 @default.
- W2124061356 cites W2127540087 @default.
- W2124061356 cites W2131212168 @default.
- W2124061356 cites W2133013000 @default.
- W2124061356 cites W2134143935 @default.
- W2124061356 cites W2137036621 @default.
- W2124061356 cites W2139368993 @default.
- W2124061356 cites W2142805668 @default.
- W2124061356 cites W2144623802 @default.
- W2124061356 cites W2145932909 @default.
- W2124061356 cites W2146892790 @default.
- W2124061356 cites W2149459456 @default.
- W2124061356 cites W2150649207 @default.
- W2124061356 cites W2151672054 @default.
- W2124061356 cites W2152036273 @default.
- W2124061356 cites W2153586836 @default.
- W2124061356 cites W2155011940 @default.
- W2124061356 cites W2155264063 @default.
- W2124061356 cites W2155452725 @default.
- W2124061356 cites W2156847772 @default.
- W2124061356 cites W2157890550 @default.
- W2124061356 cites W2160248428 @default.
- W2124061356 cites W2161017601 @default.
- W2124061356 cites W2161681137 @default.
- W2124061356 cites W2162146849 @default.
- W2124061356 cites W2165618724 @default.
- W2124061356 cites W2166308827 @default.
- W2124061356 cites W2168263755 @default.
- W2124061356 cites W2168676279 @default.
- W2124061356 cites W2168909179 @default.
- W2124061356 doi "https://doi.org/10.1128/mcb.00860-12" @default.
- W2124061356 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3486173" @default.
- W2124061356 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23007156" @default.
- W2124061356 hasPublicationYear "2012" @default.
- W2124061356 type Work @default.
- W2124061356 sameAs 2124061356 @default.