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- W2124102845 abstract "Abstract Background Standardized and well-characterized genetic building blocks are a prerequisite for the convenient and reproducible assembly of novel genetic modules and devices. While numerous standardized parts exist for Escherichia coli , such tools are still missing for the Gram-positive model organism Bacillus subtilis . The goal of this study was to develop and thoroughly evaluate such a genetic toolbox. Results We developed five BioBrick-compatible integrative B. subtilis vectors by deleting unnecessary parts and removing forbidden restriction sites to allow cloning in BioBrick (RFC10) standard. Three empty backbone vectors with compatible resistance markers and integration sites were generated, allowing the stable chromosomal integration and combination of up to three different devices in one strain. In addition, two integrative reporter vectors, based on the lacZ and luxABCDE cassettes, were BioBrick-adjusted, to enable β-galactosidase and luciferase reporter assays, respectively. Four constitutive and two inducible promoters were thoroughly characterized by quantitative, time-resolved measurements. Together, these promoters cover a range of more than three orders of magnitude in promoter strength, thereby allowing a fine-tuned adjustment of cellular protein amounts. Finally, the Bacillus BioBrick Box also provides five widely used epitope tags (FLAG, His 10 , cMyc, HA, StrepII), which can be translationally fused N- or C-terminally to any protein of choice. Conclusion Our genetic toolbox contains three compatible empty integration vectors, two reporter vectors and a set of six promoters, two of them inducible. Furthermore, five different epitope tags offer convenient protein handling and detection. All parts adhere to the BioBrick standard and hence enable standardized work with B. subtilis . We believe that our well-documented and carefully evaluated Bacillus BioBrick Box represents a very useful genetic tool kit, not only for the iGEM competition but any other BioBrick-based project in B. subtilis ." @default.
- W2124102845 created "2016-06-24" @default.
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- W2124102845 date "2013-12-01" @default.
- W2124102845 modified "2023-10-13" @default.
- W2124102845 title "The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis" @default.
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- W2124102845 cites W1554918119 @default.
- W2124102845 cites W1826524642 @default.
- W2124102845 cites W1872820980 @default.
- W2124102845 cites W1953863407 @default.
- W2124102845 cites W1965740284 @default.
- W2124102845 cites W1974799146 @default.
- W2124102845 cites W1978364047 @default.
- W2124102845 cites W1982228455 @default.
- W2124102845 cites W1991642577 @default.
- W2124102845 cites W2001426985 @default.
- W2124102845 cites W2003090737 @default.
- W2124102845 cites W2005489232 @default.
- W2124102845 cites W2012430176 @default.
- W2124102845 cites W2016667239 @default.
- W2124102845 cites W2018280419 @default.
- W2124102845 cites W2025070182 @default.
- W2124102845 cites W2027965827 @default.
- W2124102845 cites W2047882868 @default.
- W2124102845 cites W2059349322 @default.
- W2124102845 cites W2060476056 @default.
- W2124102845 cites W2065820073 @default.
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- W2124102845 cites W2077460182 @default.
- W2124102845 cites W2079843145 @default.
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- W2124102845 doi "https://doi.org/10.1186/1754-1611-7-29" @default.
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