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- W2124139384 abstract "Callus and cell suspension cultures were initiated and maintained in sugarcane clone, cv. NiF4 (Saccharum spp. hybrid) and were used as starting material. These were transformed with Agrobacterium tumefaciens EHAlOl and LBA4404 carrying a binary vector pMLH7133-GUS, which contains neomycin phospho-transferase (NPTII), hygromycin phospho-transferase (HPT) and 13-glucuronidase (GUS) genes. Expression of the GUS activity suggested that co-cultivation in liquid medium is effective, and cell aggregates of suspension culture is superior to calli cultured on solid medium as a material for Agrobacterium-mediated transformation in sugarcane. In experiment 1, hygromycin-resistant, GUS-positive calli were selected from the infected cell suspension culture. In experiment 2, hygromycin-resistant, GUS-positive shoots were regenerated from the selected calli. The HPT gene was detected from these calli and shoots by PCR analysis." @default.
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- W2124139384 date "2001-01-01" @default.
- W2124139384 modified "2023-09-23" @default.
- W2124139384 title "Agrobacterium tumefaciens-mediated transformation of sugarcane using cell suspension culture with a novel method." @default.
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