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• W2124173618 endingPage "5851" @default.
• W2124173618 startingPage "5847" @default.
• W2124173618 abstract "ABSTRACT We have sequenced the entire region of DNA required for the biosynthesis of CS5 pili from enterotoxigenic Escherichia coli O115:H40 downstream of the major subunit gene, designated csfA (for coli surface factor five A). Five more open reading frames (ORFs) ( csfB , csfC , csfE , csfF , and csfD ) which are transcribed in the same direction as the major subunit and are flanked by a number of insertion sequence regions have been identified. T7 polymerase-mediated overexpression of the cloned csf ORFs confirmed protein sizes based on the DNA sequences that encode them. The expression of only the csf region in E. coli K-12 resulted in the hemagglutination of human erythrocytes and the cell surface expression of CS5 pili, suggesting that the cluster contains all necessary information for CS5 pilus biogenesis and function." @default.
• W2124173618 created "2016-06-24" @default.
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• W2124173618 date "1999-09-15" @default.
• W2124173618 modified "2023-10-16" @default.
• W2124173618 title "CS5 Pilus Biosynthesis Genes from Enterotoxigenic <i>Escherichia coli</i> O115:H40" @default.
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• W2124173618 doi "https://doi.org/10.1128/jb.181.18.5847-5851.1999" @default.
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