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• W2124211982 abstract "<h3>Objective</h3> To investigate optimal test vial (TV) volume, utility and reliability of TVs, intermediate temperature exposure (−88°C to −93°C) before cryostorage, cryostorage in nitrogen vapor (VN₂) and liquid nitrogen (LN₂), and long-term stability of VN₂ cryostorage of human semen. <h3>Design</h3> Prospective clinical laboratory study. <h3>Setting</h3> University assisted reproductive technology (ART) laboratory. <h3>Patient(s)</h3> A total of 594 patients undergoing semen analysis and cryopreservation. <h3>Intervention(s)</h3> Semen analysis, cryopreservation with different intermediate steps and in different volumes (50–1,000 μL), and long-term storage in LN₂ or VN₂. <h3>Main Outcome Measure(s)</h3> Optimal TV volume, prediction of cryosurvival (CS) in ART procedure vials (ARTVs) with pre-freeze semen parameters and TV CS, post-thaw motility after two- or three-step semen cryopreservation and cryostorage in VN₂ and LN₂. <h3>Result(s)</h3> Test vial volume of 50 μL yielded lower CS than other volumes tested. Cryosurvival of 100 μL was similar to that of larger volumes tested. An intermediate temperature exposure (−88°C to −93°C for 20 minutes) during cryopreservation did not affect post-thaw motility. Cryosurvival of TVs and ARTVs from the same ejaculate were similar. Cryosurvival of the first TV in a series of cryopreserved ejaculates was similar to and correlated with that of TVs from different ejaculates within the same patient. Cryosurvival of the first TV was correlated with subsequent ARTVs. Long-term cryostorage in VN₂ did not affect CS. <h3>Conclusion(s)</h3> This study provides experimental evidence for use of a single 100 μL TV per patient to predict CS when freezing multiple ejaculates over a short period of time (<10 days). Additionally, semen cryostorage in VN₂ provides a stable and safe environment over time." @default.
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• W2124211982 date "2015-03-01" @default.
• W2124211982 modified "2023-10-16" @default.
• W2124211982 title "Optimizing human semen cryopreservation by reducing test vial volume and repetitive test vial sampling" @default.
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• W2124211982 doi "https://doi.org/10.1016/j.fertnstert.2014.12.107" @default.
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