Matches in SemOpenAlex for { <https://semopenalex.org/work/W2124213089> ?p ?o ?g. }
- W2124213089 endingPage "22" @default.
- W2124213089 startingPage "22" @default.
- W2124213089 abstract "Bacterial adhesion and biofilm growth can cause severe biomaterial-related infections and failure of medical implants. To assess the antifouling properties of engineered coatings, advanced approaches are needed for in situ monitoring of bacterial viability and growth kinetics as the bacteria colonize a surface. Here, we present an optimized protocol for optical real-time quantification of bacterial viability. To stain living bacteria, we replaced the commonly used fluorescent dye SYTO(®) 9 with endogenously expressed eGFP, as SYTO(®) 9 inhibited bacterial growth. With the addition of nontoxic concentrations of propidium iodide (PI) to the culture medium, the fraction of live and dead bacteria could be continuously monitored by fluorescence microscopy as demonstrated here using GFP expressing Escherichia coli as model organism. The viability of bacteria was thereby monitored on untreated and bioactive dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride (DMOAC)-coated glass substrates over several hours. Pre-adsorption of the antimicrobial surfaces with serum proteins, which mimics typical protein adsorption to biomaterial surfaces upon contact with host body fluids, completely blocked the antimicrobial activity of the DMOAC surfaces as we observed the recovery of bacterial growth. Hence, this optimized eGFP/PI viability assay provides a protocol for unperturbed in situ monitoring of bacterial viability and colonization on engineered biomaterial surfaces with single-bacteria sensitivity under physiologically relevant conditions." @default.
- W2124213089 created "2016-06-24" @default.
- W2124213089 creator A5016184584 @default.
- W2124213089 creator A5040449994 @default.
- W2124213089 creator A5041730414 @default.
- W2124213089 creator A5050544735 @default.
- W2124213089 creator A5052712800 @default.
- W2124213089 creator A5066412732 @default.
- W2124213089 creator A5079934671 @default.
- W2124213089 date "2013-12-01" @default.
- W2124213089 modified "2023-09-27" @default.
- W2124213089 title "Fluorescence-based<i>in situ</i>assay to probe the viability and growth kinetics of surface-adhering and suspended recombinant bacteria" @default.
- W2124213089 cites W1540505601 @default.
- W2124213089 cites W1901110641 @default.
- W2124213089 cites W1972053765 @default.
- W2124213089 cites W1977426084 @default.
- W2124213089 cites W1980581562 @default.
- W2124213089 cites W1985786267 @default.
- W2124213089 cites W1987283891 @default.
- W2124213089 cites W1996275832 @default.
- W2124213089 cites W2000640603 @default.
- W2124213089 cites W2001872064 @default.
- W2124213089 cites W2009977841 @default.
- W2124213089 cites W2014696362 @default.
- W2124213089 cites W2031725886 @default.
- W2124213089 cites W2038186289 @default.
- W2124213089 cites W2039510774 @default.
- W2124213089 cites W2043966332 @default.
- W2124213089 cites W2047409754 @default.
- W2124213089 cites W2048931877 @default.
- W2124213089 cites W2057326780 @default.
- W2124213089 cites W2057466373 @default.
- W2124213089 cites W2057634638 @default.
- W2124213089 cites W2062383368 @default.
- W2124213089 cites W2070300719 @default.
- W2124213089 cites W2073828389 @default.
- W2124213089 cites W2077930487 @default.
- W2124213089 cites W2078720957 @default.
- W2124213089 cites W2082939222 @default.
- W2124213089 cites W2086984087 @default.
- W2124213089 cites W2095805080 @default.
- W2124213089 cites W2101871915 @default.
- W2124213089 cites W2109717868 @default.
- W2124213089 cites W2112336662 @default.
- W2124213089 cites W2112835254 @default.
- W2124213089 cites W2119975425 @default.
- W2124213089 cites W2120978086 @default.
- W2124213089 cites W2127068865 @default.
- W2124213089 cites W2132806834 @default.
- W2124213089 cites W2144440398 @default.
- W2124213089 cites W2144821695 @default.
- W2124213089 cites W2155103701 @default.
- W2124213089 cites W2158458733 @default.
- W2124213089 cites W2160675732 @default.
- W2124213089 cites W2161978916 @default.
- W2124213089 cites W2169452559 @default.
- W2124213089 cites W2169572322 @default.
- W2124213089 cites W2247764484 @default.
- W2124213089 cites W2332072553 @default.
- W2124213089 cites W4229939445 @default.
- W2124213089 cites W4238326592 @default.
- W2124213089 cites W4243574899 @default.
- W2124213089 doi "https://doi.org/10.1186/1559-4106-8-22" @default.
- W2124213089 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4243816" @default.
- W2124213089 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24706134" @default.
- W2124213089 hasPublicationYear "2013" @default.
- W2124213089 type Work @default.
- W2124213089 sameAs 2124213089 @default.
- W2124213089 citedByCount "11" @default.
- W2124213089 countsByYear W21242130892014 @default.
- W2124213089 countsByYear W21242130892015 @default.
- W2124213089 countsByYear W21242130892017 @default.
- W2124213089 countsByYear W21242130892018 @default.
- W2124213089 countsByYear W21242130892020 @default.
- W2124213089 countsByYear W21242130892021 @default.
- W2124213089 countsByYear W21242130892022 @default.
- W2124213089 countsByYear W21242130892023 @default.
- W2124213089 crossrefType "journal-article" @default.
- W2124213089 hasAuthorship W2124213089A5016184584 @default.
- W2124213089 hasAuthorship W2124213089A5040449994 @default.
- W2124213089 hasAuthorship W2124213089A5041730414 @default.
- W2124213089 hasAuthorship W2124213089A5050544735 @default.
- W2124213089 hasAuthorship W2124213089A5052712800 @default.
- W2124213089 hasAuthorship W2124213089A5066412732 @default.
- W2124213089 hasAuthorship W2124213089A5079934671 @default.
- W2124213089 hasBestOaLocation W21242130891 @default.
- W2124213089 hasConcept C104317684 @default.
- W2124213089 hasConcept C121332964 @default.
- W2124213089 hasConcept C12554922 @default.
- W2124213089 hasConcept C169274487 @default.
- W2124213089 hasConcept C17741926 @default.
- W2124213089 hasConcept C178790620 @default.
- W2124213089 hasConcept C185592680 @default.
- W2124213089 hasConcept C190283241 @default.
- W2124213089 hasConcept C202751555 @default.
- W2124213089 hasConcept C2775934118 @default.
- W2124213089 hasConcept C2778414984 @default.
- W2124213089 hasConcept C31573885 @default.