FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2124220997> ?p ?o ?g. }

• W2124220997 endingPage "319" @default.
• W2124220997 startingPage "309" @default.
• W2124220997 abstract "The characteristics of growth and synthesis of plasmid-encoded protein were studied for strains of recombinant E. coli JM103 which carried the β-lactamase gene on plasmids of different sizes. The plasmids used included the vector pUC8 and its recombinant derivatives containing varying-sized inserts of Drosophila DNA (not expressed in E. coli). Luria broth (LB) and a minimal medium (M9) supplemented in some cases with additional inorganic phosphate were used as growth media. There was no evidence of segregational instability in these experiments, where no antibiotic selection pressure was employed. Responses of the recombinant strains to variations in environmental parameters including pH, phosphate concentration in the medium, and aeration rate were examined. While the cell growth rate in LB decreased with pH in the range 7.0–8.0, the bulk β-lactamase activity was maximized at an intermediate pH. The recombinant cell growth rate decreases with increasing plasmid size in the minimal medium, while such decrease is not significant when a rich medium such as LB is used. There is an intermediate plasmid size in the range studied (2.7–8.7 kb), at which β-lactamase activity is maximum. While reduction in aeration rate (which determines the dissolved oxygen level) is detrimental for cell growth, it is beneficial for β-lactamase synthesis. The bulk β-lactamase activity therefore exhibits a maximum with respect to aeration rate. Cell growth and β-lactamase production are affected in a similar manner by phosphate concentration in the minimal medium and therefore both are maximized at the same phosphate concentration. This investigation demonstrates clearly how the production of a recombinant plasmid-encoded protein can be maximized by proper manipulation of culture conditions and how it is affected by plasmid size." @default.
• W2124220997 created "2016-06-24" @default.
• W2124220997 creator A5006422720 @default.
• W2124220997 creator A5055085105 @default.
• W2124220997 creator A5073478356 @default.
• W2124220997 date "1989-07-01" @default.
• W2124220997 modified "2023-09-23" @default.
• W2124220997 title "Expression of ?-lactamase by recombinantEscherichia coli strains containing plasmids of different sizes?effects of pH, phosphate, and dissolved oxygen" @default.
• W2124220997 cites W1451335909 @default.
• W2124220997 cites W1542093177 @default.
• W2124220997 cites W1557830462 @default.
• W2124220997 cites W1560038937 @default.
• W2124220997 cites W1570124823 @default.
• W2124220997 cites W1601687245 @default.
• W2124220997 cites W1667731728 @default.
• W2124220997 cites W1873631119 @default.
• W2124220997 cites W1926128616 @default.
• W2124220997 cites W1932091963 @default.
• W2124220997 cites W1966902953 @default.
• W2124220997 cites W1973964227 @default.
• W2124220997 cites W1977151699 @default.
• W2124220997 cites W1989676333 @default.
• W2124220997 cites W1991177095 @default.
• W2124220997 cites W1999115523 @default.
• W2124220997 cites W2008897847 @default.
• W2124220997 cites W2009674410 @default.
• W2124220997 cites W2012298927 @default.
• W2124220997 cites W2027831635 @default.
• W2124220997 cites W2044871969 @default.
• W2124220997 cites W2047929189 @default.
• W2124220997 cites W2065978184 @default.
• W2124220997 cites W2067064181 @default.
• W2124220997 cites W2068527325 @default.
• W2124220997 cites W2074294699 @default.
• W2124220997 cites W2097312418 @default.
• W2124220997 cites W2110708405 @default.
• W2124220997 cites W2115439273 @default.
• W2124220997 cites W2116680463 @default.
• W2124220997 cites W2118589035 @default.
• W2124220997 cites W2118794883 @default.
• W2124220997 cites W2119116080 @default.
• W2124220997 cites W2152813690 @default.
• W2124220997 cites W2166104965 @default.
• W2124220997 cites W2166513343 @default.
• W2124220997 cites W2172211639 @default.
• W2124220997 cites W34642594 @default.
• W2124220997 cites W4244396076 @default.
• W2124220997 doi "https://doi.org/10.1002/bit.260340306" @default.
• W2124220997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18588109" @default.
• W2124220997 hasPublicationYear "1989" @default.
• W2124220997 type Work @default.
• W2124220997 sameAs 2124220997 @default.
• W2124220997 citedByCount "71" @default.
• W2124220997 countsByYear W21242209972012 @default.
• W2124220997 countsByYear W21242209972013 @default.
• W2124220997 countsByYear W21242209972014 @default.
• W2124220997 countsByYear W21242209972015 @default.
• W2124220997 countsByYear W21242209972016 @default.
• W2124220997 countsByYear W21242209972020 @default.
• W2124220997 countsByYear W21242209972021 @default.
• W2124220997 crossrefType "journal-article" @default.
• W2124220997 hasAuthorship W2124220997A5006422720 @default.
• W2124220997 hasAuthorship W2124220997A5055085105 @default.
• W2124220997 hasAuthorship W2124220997A5073478356 @default.
• W2124220997 hasConcept C104317684 @default.
• W2124220997 hasConcept C105702510 @default.
• W2124220997 hasConcept C178790620 @default.
• W2124220997 hasConcept C185592680 @default.
• W2124220997 hasConcept C22744801 @default.
• W2124220997 hasConcept C2524010 @default.
• W2124220997 hasConcept C2777132085 @default.
• W2124220997 hasConcept C2778022156 @default.
• W2124220997 hasConcept C2778312390 @default.
• W2124220997 hasConcept C33923547 @default.
• W2124220997 hasConcept C40767141 @default.
• W2124220997 hasConcept C43617362 @default.
• W2124220997 hasConcept C540031477 @default.
• W2124220997 hasConcept C547475151 @default.
• W2124220997 hasConcept C552990157 @default.
• W2124220997 hasConcept C55493867 @default.
• W2124220997 hasConcept C86803240 @default.
• W2124220997 hasConcept C86922832 @default.
• W2124220997 hasConcept C89423630 @default.
• W2124220997 hasConceptScore W2124220997C104317684 @default.
• W2124220997 hasConceptScore W2124220997C105702510 @default.
• W2124220997 hasConceptScore W2124220997C178790620 @default.
• W2124220997 hasConceptScore W2124220997C185592680 @default.
• W2124220997 hasConceptScore W2124220997C22744801 @default.
• W2124220997 hasConceptScore W2124220997C2524010 @default.
• W2124220997 hasConceptScore W2124220997C2777132085 @default.
• W2124220997 hasConceptScore W2124220997C2778022156 @default.
• W2124220997 hasConceptScore W2124220997C2778312390 @default.
• W2124220997 hasConceptScore W2124220997C33923547 @default.
• W2124220997 hasConceptScore W2124220997C40767141 @default.
• W2124220997 hasConceptScore W2124220997C43617362 @default.
• W2124220997 hasConceptScore W2124220997C540031477 @default.
• W2124220997 hasConceptScore W2124220997C547475151 @default.
• W2124220997 hasConceptScore W2124220997C552990157 @default.

• next