FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2124251007> ?p ?o ?g. }

• W2124251007 endingPage "202" @default.
• W2124251007 startingPage "191" @default.
• W2124251007 abstract "This article discusses the features of a newly developed matrix-assisted laser desorption/ionization quadrupole/time-of-flight (MALDI-QqTOF) mass spectrometer that is useful in the analysis of phosphorylated peptides. Aliquots of β-casein, a commonly used phosphorylated protein standard, were digested with trypsin directly on a non-porous polyurethane membrane used as sample support in MALDI-QqTOF mass spectrometry (MS) experiments. Although a complete peptide map was obtained, it was difficult to obtain sequence information for some of the tryptic fragments, in particular T1-2, which bears four phosphate groups and is thus difficult to ionize in positive mode. This article focuses on the sequencing of this particular fragment by comparing MS/MS spectra obtained using different precursor ions. These precursors associated with T1-2 were [M + H]+, [M + H]2+, and [M + H − nH3PO4]+ ions. Typically, phosphorylated ions showed facile unimolecular losses of phosphoric acid moieties, and produced limited backbone fragmentation. The abundance of [M + H]2+ ions of T1-2 in the full mass spectrum was low relative to that of [M + H]+. [M + H − 4H3PO4]+ ions as MS/MS precursors underwent backbone fragmentations, with phosphoserine residues transformed into dehydroalanines or serines. Unusual b + 18 u fragments were observed, although only for segments with previously phosphorylated serines. These partly interfered with c-ions, and were noticeable due to overlapping isotopic envelopes. It was possible to establish the sequence of phosphorylated tryptic fragment T1-2 and the location of phosphate groups using the mass of dehydroalanine residues (69 Da) and b + 18 u fragments as markers. All MS and MS/MS spectra obtained with fully phosphorylated β-casein were compared with spectra acquired with dephosphorylated β-casein obtained commercially. These comparisons helped assess the spectral differences caused by the presence of phosphate groups. Also, they highlighted the potential usefulness of conducting dephosphorylation directly on the probe prior to MALDI analysis in future studies. Copyright © 2001 John Wiley & Sons, Ltd." @default.
• W2124251007 created "2016-06-24" @default.
• W2124251007 creator A5001861722 @default.
• W2124251007 creator A5002999559 @default.
• W2124251007 creator A5030577242 @default.
• W2124251007 creator A5058370384 @default.
• W2124251007 creator A5062391667 @default.
• W2124251007 creator A5077797708 @default.
• W2124251007 creator A5090987760 @default.
• W2124251007 date "2001-01-01" @default.
• W2124251007 modified "2023-10-17" @default.
• W2124251007 title "On-membrane digestion of ?-casein for determination of phosphorylation sites by matrix-assisted laser desorption/ionization quadrupole/time-of-flight mass spectrometry" @default.
• W2124251007 cites W1964504843 @default.
• W2124251007 cites W2002609852 @default.
• W2124251007 cites W2007425565 @default.
• W2124251007 cites W2020209954 @default.
• W2124251007 cites W2028224654 @default.
• W2124251007 cites W2032318346 @default.
• W2124251007 cites W2032515511 @default.
• W2124251007 cites W2032618552 @default.
• W2124251007 cites W2041586217 @default.
• W2124251007 cites W2046205199 @default.
• W2124251007 cites W2050382345 @default.
• W2124251007 cites W2060518145 @default.
• W2124251007 cites W2069433087 @default.
• W2124251007 cites W2069698030 @default.
• W2124251007 cites W2071106732 @default.
• W2124251007 cites W2073292063 @default.
• W2124251007 cites W2075045273 @default.
• W2124251007 cites W2080960719 @default.
• W2124251007 cites W2092882300 @default.
• W2124251007 cites W2093621392 @default.
• W2124251007 cites W2117962961 @default.
• W2124251007 cites W2441730539 @default.
• W2124251007 cites W4251700885 @default.
• W2124251007 doi "https://doi.org/10.1002/1097-0231(20010215)15:3<191::aid-rcm209>3.0.co;2-n" @default.
• W2124251007 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11180550" @default.
• W2124251007 hasPublicationYear "2001" @default.
• W2124251007 type Work @default.
• W2124251007 sameAs 2124251007 @default.
• W2124251007 citedByCount "31" @default.
• W2124251007 countsByYear W21242510072012 @default.
• W2124251007 crossrefType "journal-article" @default.
• W2124251007 hasAuthorship W2124251007A5001861722 @default.
• W2124251007 hasAuthorship W2124251007A5002999559 @default.
• W2124251007 hasAuthorship W2124251007A5030577242 @default.
• W2124251007 hasAuthorship W2124251007A5058370384 @default.
• W2124251007 hasAuthorship W2124251007A5062391667 @default.
• W2124251007 hasAuthorship W2124251007A5077797708 @default.
• W2124251007 hasAuthorship W2124251007A5090987760 @default.
• W2124251007 hasConcept C111919701 @default.
• W2124251007 hasConcept C113196181 @default.
• W2124251007 hasConcept C145148216 @default.
• W2124251007 hasConcept C150394285 @default.
• W2124251007 hasConcept C162356407 @default.
• W2124251007 hasConcept C162711632 @default.
• W2124251007 hasConcept C16291436 @default.
• W2124251007 hasConcept C167050937 @default.
• W2124251007 hasConcept C178790620 @default.
• W2124251007 hasConcept C185592680 @default.
• W2124251007 hasConcept C191015642 @default.
• W2124251007 hasConcept C198291218 @default.
• W2124251007 hasConcept C202157981 @default.
• W2124251007 hasConcept C2779281246 @default.
• W2124251007 hasConcept C2779933727 @default.
• W2124251007 hasConcept C31827203 @default.
• W2124251007 hasConcept C40325409 @default.
• W2124251007 hasConcept C40684141 @default.
• W2124251007 hasConcept C41008148 @default.
• W2124251007 hasConcept C43617362 @default.
• W2124251007 hasConcept C55493867 @default.
• W2124251007 hasConcept C75280812 @default.
• W2124251007 hasConceptScore W2124251007C111919701 @default.
• W2124251007 hasConceptScore W2124251007C113196181 @default.
• W2124251007 hasConceptScore W2124251007C145148216 @default.
• W2124251007 hasConceptScore W2124251007C150394285 @default.
• W2124251007 hasConceptScore W2124251007C162356407 @default.
• W2124251007 hasConceptScore W2124251007C162711632 @default.
• W2124251007 hasConceptScore W2124251007C16291436 @default.
• W2124251007 hasConceptScore W2124251007C167050937 @default.
• W2124251007 hasConceptScore W2124251007C178790620 @default.
• W2124251007 hasConceptScore W2124251007C185592680 @default.
• W2124251007 hasConceptScore W2124251007C191015642 @default.
• W2124251007 hasConceptScore W2124251007C198291218 @default.
• W2124251007 hasConceptScore W2124251007C202157981 @default.
• W2124251007 hasConceptScore W2124251007C2779281246 @default.
• W2124251007 hasConceptScore W2124251007C2779933727 @default.
• W2124251007 hasConceptScore W2124251007C31827203 @default.
• W2124251007 hasConceptScore W2124251007C40325409 @default.
• W2124251007 hasConceptScore W2124251007C40684141 @default.
• W2124251007 hasConceptScore W2124251007C41008148 @default.
• W2124251007 hasConceptScore W2124251007C43617362 @default.
• W2124251007 hasConceptScore W2124251007C55493867 @default.
• W2124251007 hasConceptScore W2124251007C75280812 @default.
• W2124251007 hasIssue "3" @default.
• W2124251007 hasLocation W21242510071 @default.
• W2124251007 hasLocation W21242510072 @default.
• W2124251007 hasOpenAccess W2124251007 @default.

• next