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• W2124328463 abstract "The soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) proteins are small and abundant membrane-bound proteins, whose specific interactions mediate membrane fusion during cell fusion or cellular trafficking. In this study, we report the use of a label-free method, called imaging ellipsometer to analyze the interactions among three SNAREs, namely Sec22p, Ykt6p and Sso2p. The SNAREs were immobilized on the silicon wafer and then analyzed in a pairwise mode with microfluidic array, leading us to discover the interactions between Ykt6p and Sso2p, Sec22p and Sso2p. Moreover, by using the real-time function of the imaging ellipsometer, we were able to obtain their association constants (K(A)) of about 10(4) M(-1). We argue that the use of imaging ellipsometer coupled with microfluidic device will deepen our understanding of the molecular mechanisms underlying membrane fusion process." @default.
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• W2124328463 date "2014-06-18" @default.
• W2124328463 modified "2023-10-01" @default.
• W2124328463 title "Analysis of interactions between SNARE proteins using imaging ellipsometer coupled with microfluidic array" @default.
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• W2124328463 doi "https://doi.org/10.1038/srep05341" @default.
• W2124328463 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4061542" @default.
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