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- W2124381559 abstract "Considerable progress in identifying transport systems of the plant plasma membrane has been made recently. The putative systems cloned to date comprise H+-ATPases, potassium, chloride and water channels, and carriers involved in the transport of glucose, sucrose, amino acids, peptides, potassium, nitrate, ammonium, phosphate, sulfate, iron and copper. Most of these systems were identified first in Arabidopsis thaliana. Successful cloning strategies have involved the following variety of techniques: complementation of yeast mutants, screening of Arabidopsis mutants, immunoscreening of a cDNA expression library expressed in mammalian cells, screening of genomic and cDNA libraries with probes (or degenerate oligonucleotides) derived from yeast and/or animal genes, or database screening for sequence similarity to eukaryotic counterparts. Many related transport systems have subsequently been identified either by screening libraries directly, or by systematic cDNA sequencing programs. Surprisingly large gene families have been revealed. Heterologous expression systems, such as yeast, Xenopus oocytes or insect cells, provide tools for studying the transport activities, biochemical properties and structure-function relationships of these systems. Their expression and functions in planta are investigated using northern blot analysis, in situ hybridization, and transgenic approaches. Individual systems encoded by the same gene family can differ in their transport properties and have distinct tissue expression patterns. Such diversity might be central to the integration of solute transport at the whole plant level, allowing the differential expression of sets of transport systems specifically tailored to the requirements of each tissue." @default.
- W2124381559 created "2016-06-24" @default.
- W2124381559 creator A5022783575 @default.
- W2124381559 creator A5028143960 @default.
- W2124381559 creator A5036442661 @default.
- W2124381559 creator A5048715255 @default.
- W2124381559 date "1997-05-01" @default.
- W2124381559 modified "2023-10-15" @default.
- W2124381559 title "Plasma membrane transport systems in higher plants: From black boxes to molecular physiology" @default.
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