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- W2124384769 abstract "In order to ascertain whether chemical alterations in cell surface glycopeptides may be associated with tumor progression, we isolated sialogylcopeptide fractions from the surface of AS-30D rat ascites hepatoma cells before and after tumor progression. During the 70th to 90th transplantation generation of the AS-30D tumor, a morphological change and an increase in tumor virulence were observed. The tumor morphology progressed from a predominance of very large cell clusters to a predominance of single cells, doublets, triplets, and smaller clusters. The neuraminidase-labile, cell surface sialic acid of the AS-30D tumor decreased 27% after morphological progression of the tumor. Sialoglycopeptide fractions were prepared by papain digestion of cell suspensions obtained from the 11th to 14th, 49th to 55th, and 116th transplantation generations. These fractions were partially purified by trichloroacetic acid precipitation, dialysis, and gel filtration on Sephadex G-50. Compositional analysis and ion-exchange chromatographic behavior of the sialoglycopeptide fractions were similar except for a marked decrease in their glucose content associated with increased passage of the tumor." @default.
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- W2124384769 date "1972-03-01" @default.
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- W2124384769 title "Isolation and chemical characterization of cell surface sialoglycopeptide fractions during progression of rat ascites hepatoma AS-30D." @default.
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