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• W2124402246 abstract "ABSTRACT Detection of antigen-specific CD4 + T cells is facilitated by the use of fluorescently labeled soluble peptide-major histocompatibility complex (MHC) multimers which mirror the antigen specificity of T-cell receptor recognition. We have used soluble peptide-MHC class II tetramers containing peptides from the protective antigen (PA) of Bacillus anthracis to detect circulating T cells in peripheral blood of subjects vaccinated with an anthrax vaccine. PA-specific HLA class II-restricted T lymphocytes were isolated which displayed both TH1- and TH2-like characteristics, indicating heterogeneity of the lymphocyte lineage within the CD4 + response. Presentation of antigen to these T-cell clones by HLA-matched antigen-presenting cells exposed to the intact PA protein confirmed that the identified epitopes are indeed naturally processed by the human immune system. Specific tetramer-derived T-cell profiling may be useful for monitoring helper CD4 + T-cell responses to anthrax vaccination." @default.
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• W2124402246 date "2007-04-01" @default.
• W2124402246 modified "2023-10-17" @default.
• W2124402246 title "Antigen-Specific CD4 ⁺ T Cells Recognize Epitopes of Protective Antigen following Vaccination with an Anthrax Vaccine" @default.
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• W2124402246 doi "https://doi.org/10.1128/iai.01814-06" @default.
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