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- W2124451323 abstract "Strains of Xanthomonas campestris pv. vesicatoria (Xcv) carrying avrBs2 are specifically recognized by Bs2 pepper plants, resulting in localized cell death and plant resistance. Agrobacterium-mediated transient expression of the Xcv avrBs2 gene in plant cells results in Bs2-dependent cell death, indicating that the AvrBs2 protein alone is sufficient for the activation of disease resistance-mediated cell death in planta. We now provide evidence that AvrBs2 is secreted from Xcv and that secretion is type III (hrp) dependent. N- and C-terminal deletion analysis of AvrBs2 has identified the effector domain of AvrBs2 recognized by Bs2 pepper plants. By using a truncated Pseudomonas syringae AvrRpt2 effector reporter devoid of type III signal sequences, we have localized the minimal region of AvrBs2 required for type III secretion in Xcv. Furthermore, we have identified the region of AvrBs2 required for both type III secretion and translocation to host plants. The mapping of AvrBs2 sequences sufficient for type III delivery also revealed the presence of a potential mRNA secretion signal." @default.
- W2124451323 created "2016-06-24" @default.
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- W2124451323 date "2000-11-14" @default.
- W2124451323 modified "2023-09-27" @default.
- W2124451323 title "Molecular signals required for type III secretion and translocation of the <i>Xanthomonas campestris</i> AvrBs2 protein to pepper plants" @default.
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- W2124451323 doi "https://doi.org/10.1073/pnas.230450797" @default.
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