Matches in SemOpenAlex for { <https://semopenalex.org/work/W2124456479> ?p ?o ?g. }
- W2124456479 endingPage "82" @default.
- W2124456479 startingPage "70" @default.
- W2124456479 abstract "Abstract A robust DNA extraction method is important to identify the majority of microorganisms present in environmental microbial communities and to enable a consistent comparison between different studies. Here, 15 manual and four automated commercial DNA extraction kits were evaluated for their efficiency to extract DNA from porcine feces and ileal digesta samples. DNA yield, integrity, and purity varied among the different methods. Terminal restriction fragment length polymorphism (T‐ RFLP ) and Illumina amplicon sequencing were used to characterize the diversity and composition of the microbial communities. We also compared phylogenetic profiles of two regions of the 16S rRNA gene, one of the most used region (V1–2) and the V5–6 region. A high correlation between community structures obtained by analyzing both regions was observed at genus and family level for ileum digesta and feces. Based on our findings, we want to recommend the Fast DNA ™ SPIN Kit for Soil ( MP Biomedical) as a suitable kit for the analyses of porcine gastrointestinal tract samples." @default.
- W2124456479 created "2016-06-24" @default.
- W2124456479 creator A5025297319 @default.
- W2124456479 creator A5027219705 @default.
- W2124456479 creator A5035474246 @default.
- W2124456479 creator A5064220909 @default.
- W2124456479 date "2015-11-05" @default.
- W2124456479 modified "2023-10-11" @default.
- W2124456479 title "Evaluation of <scp>DNA</scp> extraction kits and phylogenetic diversity of the porcine gastrointestinal tract based on Illumina sequencing of two hypervariable regions" @default.
- W2124456479 cites W1764295403 @default.
- W2124456479 cites W1852407256 @default.
- W2124456479 cites W1966355274 @default.
- W2124456479 cites W1967850793 @default.
- W2124456479 cites W1969435169 @default.
- W2124456479 cites W1975301516 @default.
- W2124456479 cites W1981029671 @default.
- W2124456479 cites W1987557410 @default.
- W2124456479 cites W1989947520 @default.
- W2124456479 cites W1994441172 @default.
- W2124456479 cites W2001367223 @default.
- W2124456479 cites W2006069224 @default.
- W2124456479 cites W2011223551 @default.
- W2124456479 cites W2014141043 @default.
- W2124456479 cites W2014206657 @default.
- W2124456479 cites W2015309066 @default.
- W2124456479 cites W2021345151 @default.
- W2124456479 cites W2027588231 @default.
- W2124456479 cites W2051246865 @default.
- W2124456479 cites W2051322058 @default.
- W2124456479 cites W2057090852 @default.
- W2124456479 cites W2061016076 @default.
- W2124456479 cites W2065842063 @default.
- W2124456479 cites W2069752064 @default.
- W2124456479 cites W2079397498 @default.
- W2124456479 cites W2085691101 @default.
- W2124456479 cites W2092431346 @default.
- W2124456479 cites W2092466285 @default.
- W2124456479 cites W2093229186 @default.
- W2124456479 cites W2095132285 @default.
- W2124456479 cites W2101960580 @default.
- W2124456479 cites W2104142566 @default.
- W2124456479 cites W2122454567 @default.
- W2124456479 cites W2122767606 @default.
- W2124456479 cites W2125826054 @default.
- W2124456479 cites W2131415145 @default.
- W2124456479 cites W2139784934 @default.
- W2124456479 cites W2143539303 @default.
- W2124456479 cites W2144324631 @default.
- W2124456479 cites W2144568881 @default.
- W2124456479 cites W2147494764 @default.
- W2124456479 cites W2147899248 @default.
- W2124456479 cites W2149426290 @default.
- W2124456479 cites W2151159165 @default.
- W2124456479 cites W2151547579 @default.
- W2124456479 cites W2152885278 @default.
- W2124456479 cites W2157783441 @default.
- W2124456479 cites W2166095584 @default.
- W2124456479 cites W2168634626 @default.
- W2124456479 cites W2323021532 @default.
- W2124456479 doi "https://doi.org/10.1002/mbo3.312" @default.
- W2124456479 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4767427" @default.
- W2124456479 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26541370" @default.
- W2124456479 hasPublicationYear "2015" @default.
- W2124456479 type Work @default.
- W2124456479 sameAs 2124456479 @default.
- W2124456479 citedByCount "52" @default.
- W2124456479 countsByYear W21244564792016 @default.
- W2124456479 countsByYear W21244564792017 @default.
- W2124456479 countsByYear W21244564792018 @default.
- W2124456479 countsByYear W21244564792019 @default.
- W2124456479 countsByYear W21244564792020 @default.
- W2124456479 countsByYear W21244564792021 @default.
- W2124456479 countsByYear W21244564792022 @default.
- W2124456479 countsByYear W21244564792023 @default.
- W2124456479 crossrefType "journal-article" @default.
- W2124456479 hasAuthorship W2124456479A5025297319 @default.
- W2124456479 hasAuthorship W2124456479A5027219705 @default.
- W2124456479 hasAuthorship W2124456479A5035474246 @default.
- W2124456479 hasAuthorship W2124456479A5064220909 @default.
- W2124456479 hasBestOaLocation W21244564791 @default.
- W2124456479 hasConcept C104317684 @default.
- W2124456479 hasConcept C106337198 @default.
- W2124456479 hasConcept C168007829 @default.
- W2124456479 hasConcept C171636592 @default.
- W2124456479 hasConcept C193252679 @default.
- W2124456479 hasConcept C42062724 @default.
- W2124456479 hasConcept C49105822 @default.
- W2124456479 hasConcept C51679486 @default.
- W2124456479 hasConcept C54355233 @default.
- W2124456479 hasConcept C552990157 @default.
- W2124456479 hasConcept C61716771 @default.
- W2124456479 hasConcept C70721500 @default.
- W2124456479 hasConcept C78063203 @default.
- W2124456479 hasConcept C81566733 @default.
- W2124456479 hasConcept C8185291 @default.
- W2124456479 hasConcept C86803240 @default.
- W2124456479 hasConcept C89423630 @default.
- W2124456479 hasConceptScore W2124456479C104317684 @default.