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• W2124459170 endingPage "5028" @default.
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• W2124459170 abstract "ABSTRACT Human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) strains differ in their capacity to replicate in macrophages, but mechanisms underlying these differences are not fully understood. Here, we identify a highly conserved N-linked glycosylation site (N173 in SIV, corresponding to N160 in HIV) in the V2 region of the SIV envelope glycoprotein (Env) as a novel determinant of macrophage tropism and characterize mechanisms underlying this phenotype. Loss of the N173 glycosylation site in the non-macrophage-tropic SIVmac239 by introducing an N173Q mutation enhanced viral replication and multinucleated giant cell formation upon infection of rhesus macrophages, while the addition of N173 to SIVmac251 had the opposite effect. The removal of N173 in SIVmac239 enhanced CD4-independent cell-to-cell transmission to CCR5-expressing cells. SIVmac239 with N173Q mediated CD4-independent cell-cell fusion but could not infect CD4-negative cells in single-round infections. Thus, CD4-independent phenotypes were detected only in the context of cell-to-cell contact. Similar results were obtained in SIVmac251 with and without N173. N173 decreased the neutralization sensitivity of SIVmac251 but had no effect on the neutralization sensitivity of SIVmac239. The N173Q mutation had no effect on SIVmac239 binding to CD4 in Biacore assays, coimmunoprecipitation assays, and enzyme-linked immunosorbent assays (ELISAs). These findings suggest that the loss of the N173 N-linked glycosylation site increases SIVmac239 replication in macrophages by enhancing CD4-independent cell-to-cell virus transmission through CCR5-mediated fusion. This mechanism may facilitate the escape of macrophage-tropic viruses from neutralizing antibodies while promoting spreading infection by these viruses in vivo . IMPORTANCE In this study, we identify a genetic determinant in the viral envelope (N173) that increases replication and spreading infection of SIV strains in macrophages by enhancing cell-to-cell virus transmission. This effect is explained by a novel mechanism involving increased cell-to-cell fusion in the absence of CD4, the primary receptor that normally mediates virus entry. The same genetic determinant also affects the sensitivity of these viruses to inhibition by neutralizing antibodies. Most macrophage-tropic HIV/SIV strains are known to be neutralization sensitive. Together, these findings suggest that this efficient mode of virus transmission may facilitate the escape of macrophage-tropic viruses from neutralizing antibodies while promoting spreading infection by these viruses to cells expressing little or no CD4 in vivo ." @default.
• W2124459170 created "2016-06-24" @default.
• W2124459170 creator A5018901631 @default.
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• W2124459170 creator A5066679273 @default.
• W2124459170 creator A5071476197 @default.
• W2124459170 creator A5075224434 @default.
• W2124459170 creator A5081520991 @default.
• W2124459170 date "2014-05-01" @default.
• W2124459170 modified "2023-09-23" @default.
• W2124459170 title "Loss of a Conserved N-Linked Glycosylation Site in the Simian Immunodeficiency Virus Envelope Glycoprotein V2 Region Enhances Macrophage Tropism by Increasing CD4-Independent Cell-to-Cell Transmission" @default.
• W2124459170 cites W1483105582 @default.
• W2124459170 cites W1493470785 @default.
• W2124459170 cites W1532310879 @default.
• W2124459170 cites W1553637501 @default.
• W2124459170 cites W1607434342 @default.
• W2124459170 cites W1628484047 @default.
• W2124459170 cites W1964017631 @default.
• W2124459170 cites W1965495859 @default.
• W2124459170 cites W1966607693 @default.
• W2124459170 cites W1970563411 @default.
• W2124459170 cites W1971553870 @default.
• W2124459170 cites W1972341594 @default.
• W2124459170 cites W1975634384 @default.
• W2124459170 cites W1977929936 @default.
• W2124459170 cites W1983365822 @default.
• W2124459170 cites W1987393778 @default.
• W2124459170 cites W1993834400 @default.
• W2124459170 cites W1995355944 @default.
• W2124459170 cites W1996061504 @default.
• W2124459170 cites W1996684522 @default.
• W2124459170 cites W1999053028 @default.
• W2124459170 cites W1999483871 @default.
• W2124459170 cites W2000884295 @default.
• W2124459170 cites W2000885937 @default.
• W2124459170 cites W2002896157 @default.
• W2124459170 cites W2003264913 @default.
• W2124459170 cites W2003797832 @default.
• W2124459170 cites W2012816187 @default.
• W2124459170 cites W2016180326 @default.
• W2124459170 cites W2018930937 @default.
• W2124459170 cites W2023398760 @default.
• W2124459170 cites W2023604714 @default.
• W2124459170 cites W2025302903 @default.
• W2124459170 cites W2031983597 @default.
• W2124459170 cites W2031992914 @default.
• W2124459170 cites W2032410170 @default.
• W2124459170 cites W2032614798 @default.
• W2124459170 cites W2039642538 @default.
• W2124459170 cites W2042476299 @default.
• W2124459170 cites W2050070282 @default.
• W2124459170 cites W2053377271 @default.
• W2124459170 cites W2054564712 @default.
• W2124459170 cites W2058632331 @default.
• W2124459170 cites W2062204035 @default.
• W2124459170 cites W2064268252 @default.
• W2124459170 cites W2067107443 @default.
• W2124459170 cites W2071516463 @default.
• W2124459170 cites W2072003614 @default.
• W2124459170 cites W2073044742 @default.
• W2124459170 cites W2082091832 @default.
• W2124459170 cites W2083726777 @default.
• W2124459170 cites W2089692155 @default.
• W2124459170 cites W2094158458 @default.
• W2124459170 cites W2095703604 @default.
• W2124459170 cites W2095939962 @default.
• W2124459170 cites W2099171570 @default.
• W2124459170 cites W2099311529 @default.
• W2124459170 cites W2099381548 @default.
• W2124459170 cites W2099576234 @default.
• W2124459170 cites W2100027422 @default.
• W2124459170 cites W2101926858 @default.
• W2124459170 cites W2104802051 @default.
• W2124459170 cites W2107298174 @default.
• W2124459170 cites W2113236498 @default.
• W2124459170 cites W2120031187 @default.
• W2124459170 cites W2120520610 @default.
• W2124459170 cites W2122531531 @default.
• W2124459170 cites W2122617162 @default.
• W2124459170 cites W2124832895 @default.
• W2124459170 cites W2124935744 @default.
• W2124459170 cites W2128579789 @default.
• W2124459170 cites W2130565519 @default.
• W2124459170 cites W2131692078 @default.
• W2124459170 cites W2133629725 @default.
• W2124459170 cites W2133956851 @default.
• W2124459170 cites W2136000813 @default.
• W2124459170 cites W2136958613 @default.
• W2124459170 cites W2137366946 @default.
• W2124459170 cites W2137428749 @default.
• W2124459170 cites W2137903260 @default.
• W2124459170 cites W2141489451 @default.
• W2124459170 cites W2142035308 @default.
• W2124459170 cites W2143421242 @default.
• W2124459170 cites W2143614051 @default.
• W2124459170 cites W2146426059 @default.
• W2124459170 cites W2152281378 @default.

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