Matches in SemOpenAlex for { <https://semopenalex.org/work/W2124492012> ?p ?o ?g. }
- W2124492012 endingPage "4199" @default.
- W2124492012 startingPage "4192" @default.
- W2124492012 abstract "ABSTRACT The human pathogen enterohemorrhagic Escherichia coli (EHEC) O157:H7 colonizes the rectoanal junction (RAJ) in cattle, its natural reservoir. Colonization at the RAJ poses a serious risk for fecal shedding and contamination of the environment. We previously demonstrated that EHEC senses acyl-homoserine lactones (AHLs) produced by the microbiota in the rumen to activate the gad acid resistance genes necessary for survival through the acidic stomachs in cattle and to repress the locus of enterocyte effacement (LEE) genes important for colonization of the RAJ, but unnecessary in the rumen. Devoid of AHLs, the RAJ is the prominent site of colonization of EHEC in cattle. To determine if the presence of AHLs in the RAJ could repress colonization at this site, we engineered EHEC to express the Yersinia enterocolitica AHL synthase gene yenI , which constitutively produces AHLs, to mimic a constant exposure of AHLs in the environment. The yenI + EHEC produces oxo-C6-homoserine lactone (oxo-C6-HSL) and had a significant reduction in LEE expression, effector protein secretion, and attaching and effacing (A/E) lesion formation in vitro compared to the wild type (WT). The yenI + EHEC also activated expression of the gad genes. To assess whether AHL production, which decreases LEE expression, would decrease RAJ colonization by EHEC, cattle were challenged at the RAJ with WT or yenI + EHEC. Although the yenI + EHEC colonized the RAJ with efficiency equal to that of the WT, there was a trend for the cattle to shed the WT strain longer than the yenI + EHEC." @default.
- W2124492012 created "2016-06-24" @default.
- W2124492012 creator A5004199630 @default.
- W2124492012 creator A5008162118 @default.
- W2124492012 creator A5019486706 @default.
- W2124492012 creator A5027665615 @default.
- W2124492012 creator A5061511885 @default.
- W2124492012 creator A5077198825 @default.
- W2124492012 date "2013-11-01" @default.
- W2124492012 modified "2023-10-16" @default.
- W2124492012 title "The Acyl-Homoserine Lactone Synthase YenI from Yersinia enterocolitica Modulates Virulence Gene Expression in Enterohemorrhagic Escherichia coli O157:H7" @default.
- W2124492012 cites W1492600174 @default.
- W2124492012 cites W1588164239 @default.
- W2124492012 cites W1895584462 @default.
- W2124492012 cites W1904815041 @default.
- W2124492012 cites W1922086601 @default.
- W2124492012 cites W1968294861 @default.
- W2124492012 cites W1970347595 @default.
- W2124492012 cites W1974566785 @default.
- W2124492012 cites W1978376077 @default.
- W2124492012 cites W1979633235 @default.
- W2124492012 cites W1983344225 @default.
- W2124492012 cites W1985396638 @default.
- W2124492012 cites W1987436161 @default.
- W2124492012 cites W1992263339 @default.
- W2124492012 cites W1997961331 @default.
- W2124492012 cites W2002375686 @default.
- W2124492012 cites W2027659554 @default.
- W2124492012 cites W2032306900 @default.
- W2124492012 cites W2038309376 @default.
- W2124492012 cites W2040641257 @default.
- W2124492012 cites W2043302089 @default.
- W2124492012 cites W2045594930 @default.
- W2124492012 cites W2051347791 @default.
- W2124492012 cites W2052967851 @default.
- W2124492012 cites W2060990894 @default.
- W2124492012 cites W2061060437 @default.
- W2124492012 cites W2068174324 @default.
- W2124492012 cites W2073376843 @default.
- W2124492012 cites W2095011510 @default.
- W2124492012 cites W2097393614 @default.
- W2124492012 cites W2099768150 @default.
- W2124492012 cites W2102382756 @default.
- W2124492012 cites W2103444887 @default.
- W2124492012 cites W2111602293 @default.
- W2124492012 cites W2112111992 @default.
- W2124492012 cites W2112150569 @default.
- W2124492012 cites W2112344221 @default.
- W2124492012 cites W2114682122 @default.
- W2124492012 cites W2115489314 @default.
- W2124492012 cites W2116137883 @default.
- W2124492012 cites W2119856153 @default.
- W2124492012 cites W2128896236 @default.
- W2124492012 cites W2135894069 @default.
- W2124492012 cites W2138004396 @default.
- W2124492012 cites W2138776000 @default.
- W2124492012 cites W2141240337 @default.
- W2124492012 cites W2144963709 @default.
- W2124492012 cites W2146730213 @default.
- W2124492012 cites W2148396729 @default.
- W2124492012 cites W2149147672 @default.
- W2124492012 cites W2151592044 @default.
- W2124492012 cites W2151933243 @default.
- W2124492012 cites W2156080241 @default.
- W2124492012 cites W2156270624 @default.
- W2124492012 cites W2158266769 @default.
- W2124492012 cites W2159246434 @default.
- W2124492012 cites W2160403140 @default.
- W2124492012 cites W2164259487 @default.
- W2124492012 cites W2165619858 @default.
- W2124492012 cites W2166127814 @default.
- W2124492012 cites W2168190685 @default.
- W2124492012 cites W2171006179 @default.
- W2124492012 cites W2171228833 @default.
- W2124492012 cites W2172946412 @default.
- W2124492012 cites W2335304797 @default.
- W2124492012 doi "https://doi.org/10.1128/iai.00889-13" @default.
- W2124492012 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3811827" @default.
- W2124492012 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23980115" @default.
- W2124492012 hasPublicationYear "2013" @default.
- W2124492012 type Work @default.
- W2124492012 sameAs 2124492012 @default.
- W2124492012 citedByCount "9" @default.
- W2124492012 countsByYear W21244920122015 @default.
- W2124492012 countsByYear W21244920122018 @default.
- W2124492012 countsByYear W21244920122019 @default.
- W2124492012 countsByYear W21244920122020 @default.
- W2124492012 countsByYear W21244920122022 @default.
- W2124492012 countsByYear W21244920122023 @default.
- W2124492012 crossrefType "journal-article" @default.
- W2124492012 hasAuthorship W2124492012A5004199630 @default.
- W2124492012 hasAuthorship W2124492012A5008162118 @default.
- W2124492012 hasAuthorship W2124492012A5019486706 @default.
- W2124492012 hasAuthorship W2124492012A5027665615 @default.
- W2124492012 hasAuthorship W2124492012A5061511885 @default.
- W2124492012 hasAuthorship W2124492012A5077198825 @default.
- W2124492012 hasBestOaLocation W21244920121 @default.
- W2124492012 hasConcept C104317684 @default.