FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2124548832> ?p ?o ?g. }

• W2124548832 endingPage "288" @default.
• W2124548832 startingPage "277" @default.
• W2124548832 abstract "Excessive proteolytic degradation of fibronectin (FN) has been implicated in impaired tissue repair in chronic wounds. We previously reported two strategies for stabilizing FN against proteolytic degradation; the first conjugated polyethylene glycol (PEG) through cysteine residues and the second conjugated PEG chains of varying molecular weight on lysine residues. PEGylation of FN via lysine residues resulted in increased resistance to proteolysis with increasing PEG size, but an overall decrease in biological activity, as characterized by cell and gelatin binding. Our latest method to stabilize FN against proteolysis masks functional regions in the protein during lysine PEGylation. FN is PEGylated while it is bound to gelatin Sepharose beads with 2, 5, and 10 kDa PEG precursors. This results in partially PEGylated FN that is more stable than native FN and whose proteolytic stability increases with PEG molecular weight. Unlike completely PEGylated FN, partially PEGylated FN has cell adhesion, gelatin binding, and matrix assembly responses that are comparable to native FN. This is new evidence of how PEGylation variables can be used to stabilize FN while retaining its activity. The conjugates developed herein can be used to dissect molecular mechanisms mediated by FN stability and functionality, and address the problem of FN degradation in chronic wounds. © 2014 American Institute of Chemical Engineers Biotechnol. Prog., 31:277–288, 2015" @default.
• W2124548832 created "2016-06-24" @default.
• W2124548832 creator A5016904892 @default.
• W2124548832 creator A5034855502 @default.
• W2124548832 creator A5038802830 @default.
• W2124548832 date "2014-11-24" @default.
• W2124548832 modified "2023-09-30" @default.
• W2124548832 title "Proteolytically stabilizing fibronectin without compromising cell and gelatin binding activity" @default.
• W2124548832 cites W140120344 @default.
• W2124548832 cites W146078149 @default.
• W2124548832 cites W1539404498 @default.
• W2124548832 cites W1550344038 @default.
• W2124548832 cites W1558451333 @default.
• W2124548832 cites W1560078646 @default.
• W2124548832 cites W1568784633 @default.
• W2124548832 cites W1573296762 @default.
• W2124548832 cites W1812258050 @default.
• W2124548832 cites W1968717450 @default.
• W2124548832 cites W1976066469 @default.
• W2124548832 cites W1977941193 @default.
• W2124548832 cites W1984947128 @default.
• W2124548832 cites W1987067083 @default.
• W2124548832 cites W2012368513 @default.
• W2124548832 cites W2015834878 @default.
• W2124548832 cites W2023644368 @default.
• W2124548832 cites W2023692836 @default.
• W2124548832 cites W2023989365 @default.
• W2124548832 cites W2024373128 @default.
• W2124548832 cites W2024413031 @default.
• W2124548832 cites W2024667292 @default.
• W2124548832 cites W2028200036 @default.
• W2124548832 cites W2029003138 @default.
• W2124548832 cites W2035802939 @default.
• W2124548832 cites W2036130205 @default.
• W2124548832 cites W2041275298 @default.
• W2124548832 cites W2043960873 @default.
• W2124548832 cites W2044694782 @default.
• W2124548832 cites W2046168396 @default.
• W2124548832 cites W2047188856 @default.
• W2124548832 cites W2050485974 @default.
• W2124548832 cites W2051256914 @default.
• W2124548832 cites W2051570308 @default.
• W2124548832 cites W2056388718 @default.
• W2124548832 cites W2068324201 @default.
• W2124548832 cites W2074405834 @default.
• W2124548832 cites W2077118080 @default.
• W2124548832 cites W2081643000 @default.
• W2124548832 cites W2092040506 @default.
• W2124548832 cites W2094704598 @default.
• W2124548832 cites W2095930245 @default.
• W2124548832 cites W2096172752 @default.
• W2124548832 cites W2104359967 @default.
• W2124548832 cites W2139629945 @default.
• W2124548832 cites W2146453842 @default.
• W2124548832 cites W2147791888 @default.
• W2124548832 cites W2151810131 @default.
• W2124548832 cites W2152931265 @default.
• W2124548832 cites W2161410789 @default.
• W2124548832 cites W2326242369 @default.
• W2124548832 cites W2407878834 @default.
• W2124548832 cites W4236272134 @default.
• W2124548832 cites W93111349 @default.
• W2124548832 doi "https://doi.org/10.1002/btpr.2018" @default.
• W2124548832 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25394993" @default.
• W2124548832 hasPublicationYear "2014" @default.
• W2124548832 type Work @default.
• W2124548832 sameAs 2124548832 @default.
• W2124548832 citedByCount "2" @default.
• W2124548832 countsByYear W21245488322019 @default.
• W2124548832 countsByYear W21245488322020 @default.
• W2124548832 crossrefType "journal-article" @default.
• W2124548832 hasAuthorship W2124548832A5016904892 @default.
• W2124548832 hasAuthorship W2124548832A5034855502 @default.
• W2124548832 hasAuthorship W2124548832A5038802830 @default.
• W2124548832 hasBestOaLocation W21245488321 @default.
• W2124548832 hasConcept C10138342 @default.
• W2124548832 hasConcept C12554922 @default.
• W2124548832 hasConcept C1491633281 @default.
• W2124548832 hasConcept C162324750 @default.
• W2124548832 hasConcept C181199279 @default.
• W2124548832 hasConcept C185592680 @default.
• W2124548832 hasConcept C2776016237 @default.
• W2124548832 hasConcept C2776964284 @default.
• W2124548832 hasConcept C2778823387 @default.
• W2124548832 hasConcept C2779149719 @default.
• W2124548832 hasConcept C2781307694 @default.
• W2124548832 hasConcept C515207424 @default.
• W2124548832 hasConcept C54400483 @default.
• W2124548832 hasConcept C55493867 @default.
• W2124548832 hasConcept C85789140 @default.
• W2124548832 hasConcept C86492073 @default.
• W2124548832 hasConcept C86803240 @default.
• W2124548832 hasConceptScore W2124548832C10138342 @default.
• W2124548832 hasConceptScore W2124548832C12554922 @default.
• W2124548832 hasConceptScore W2124548832C1491633281 @default.
• W2124548832 hasConceptScore W2124548832C162324750 @default.
• W2124548832 hasConceptScore W2124548832C181199279 @default.
• W2124548832 hasConceptScore W2124548832C185592680 @default.

• next