FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2124633033> ?p ?o ?g. }

• W2124633033 endingPage "246" @default.
• W2124633033 startingPage "240" @default.
• W2124633033 abstract "The base excision repair (BER) pathway plays a key role in protecting the genome from endogenous DNA damage. Current methods to measure BER activities are indirect and cumbersome. Here, we introduce a direct method to assay DNA excision repair that is suitable for automation and industrial use, based on the fluorescence quenching mechanism of molecular beacons. We designed a single-stranded DNA oligonucleotide labelled with a 5'-fluorescein (F) and a 3'-Dabcyl (D) in which the fluorophore, F, is held in close proximity to the quencher, D, by the stem-loop structure design of the oligonucleotide. Following removal of the modified base or incision of the oligonucleotide, the fluorophore is separated from the quencher and fluorescence can be detected as a function of time. Several modified beacons have been used to validate the assay on both cell-free extracts and purified proteins. We have further developed the method to analyze BER in cultured cells. As described, the molecular beacon-based assay can be applied to all DNA modifications processed by DNA excision/incision repair pathways. Possible applications of the assay are discussed, including high-throughput real-time DNA repair measurements both in vitro and in living cells." @default.
• W2124633033 created "2016-06-24" @default.
• W2124633033 creator A5003204998 @default.
• W2124633033 creator A5007737413 @default.
• W2124633033 creator A5038021150 @default.
• W2124633033 creator A5048406939 @default.
• W2124633033 creator A5064506934 @default.
• W2124633033 creator A5067760784 @default.
• W2124633033 date "2004-06-01" @default.
• W2124633033 modified "2023-09-25" @default.
• W2124633033 title "A molecular beacon assay for measuring base excision repair activities" @default.
• W2124633033 cites W1497294456 @default.
• W2124633033 cites W1534310027 @default.
• W2124633033 cites W1557101255 @default.
• W2124633033 cites W1601733367 @default.
• W2124633033 cites W1965228991 @default.
• W2124633033 cites W1968895302 @default.
• W2124633033 cites W1977932650 @default.
• W2124633033 cites W1991813493 @default.
• W2124633033 cites W2012707998 @default.
• W2124633033 cites W2018553092 @default.
• W2124633033 cites W2026710381 @default.
• W2124633033 cites W2026895762 @default.
• W2124633033 cites W2041683774 @default.
• W2124633033 cites W2047479283 @default.
• W2124633033 cites W2053999371 @default.
• W2124633033 cites W2056669656 @default.
• W2124633033 cites W2062253025 @default.
• W2124633033 cites W2067546483 @default.
• W2124633033 cites W2079555125 @default.
• W2124633033 cites W2079574184 @default.
• W2124633033 cites W2080982746 @default.
• W2124633033 cites W2088814535 @default.
• W2124633033 cites W2095965962 @default.
• W2124633033 cites W2096136751 @default.
• W2124633033 cites W2112773853 @default.
• W2124633033 cites W2127838106 @default.
• W2124633033 cites W2131970139 @default.
• W2124633033 cites W2135825601 @default.
• W2124633033 cites W2137095626 @default.
• W2124633033 cites W2157867429 @default.
• W2124633033 cites W2168558045 @default.
• W2124633033 cites W2169834878 @default.
• W2124633033 cites W2171409050 @default.
• W2124633033 cites W2323274627 @default.
• W2124633033 doi "https://doi.org/10.1016/j.bbrc.2004.04.179" @default.
• W2124633033 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15158468" @default.
• W2124633033 hasPublicationYear "2004" @default.
• W2124633033 type Work @default.
• W2124633033 sameAs 2124633033 @default.
• W2124633033 citedByCount "82" @default.
• W2124633033 countsByYear W21246330332012 @default.
• W2124633033 countsByYear W21246330332013 @default.
• W2124633033 countsByYear W21246330332014 @default.
• W2124633033 countsByYear W21246330332015 @default.
• W2124633033 countsByYear W21246330332016 @default.
• W2124633033 countsByYear W21246330332017 @default.
• W2124633033 countsByYear W21246330332018 @default.
• W2124633033 countsByYear W21246330332019 @default.
• W2124633033 countsByYear W21246330332020 @default.
• W2124633033 countsByYear W21246330332021 @default.
• W2124633033 countsByYear W21246330332022 @default.
• W2124633033 countsByYear W21246330332023 @default.
• W2124633033 crossrefType "journal-article" @default.
• W2124633033 hasAuthorship W2124633033A5003204998 @default.
• W2124633033 hasAuthorship W2124633033A5007737413 @default.
• W2124633033 hasAuthorship W2124633033A5038021150 @default.
• W2124633033 hasAuthorship W2124633033A5048406939 @default.
• W2124633033 hasAuthorship W2124633033A5064506934 @default.
• W2124633033 hasAuthorship W2124633033A5067760784 @default.
• W2124633033 hasConcept C104451858 @default.
• W2124633033 hasConcept C119203544 @default.
• W2124633033 hasConcept C121332964 @default.
• W2124633033 hasConcept C129312508 @default.
• W2124633033 hasConcept C134935766 @default.
• W2124633033 hasConcept C143425029 @default.
• W2124633033 hasConcept C153911025 @default.
• W2124633033 hasConcept C185592680 @default.
• W2124633033 hasConcept C187206112 @default.
• W2124633033 hasConcept C192396546 @default.
• W2124633033 hasConcept C2778617687 @default.
• W2124633033 hasConcept C552990157 @default.
• W2124633033 hasConcept C55493867 @default.
• W2124633033 hasConcept C62520636 @default.
• W2124633033 hasConcept C70721500 @default.
• W2124633033 hasConcept C86803240 @default.
• W2124633033 hasConcept C91881484 @default.
• W2124633033 hasConcept C95444343 @default.
• W2124633033 hasConceptScore W2124633033C104451858 @default.
• W2124633033 hasConceptScore W2124633033C119203544 @default.
• W2124633033 hasConceptScore W2124633033C121332964 @default.
• W2124633033 hasConceptScore W2124633033C129312508 @default.
• W2124633033 hasConceptScore W2124633033C134935766 @default.
• W2124633033 hasConceptScore W2124633033C143425029 @default.
• W2124633033 hasConceptScore W2124633033C153911025 @default.
• W2124633033 hasConceptScore W2124633033C185592680 @default.
• W2124633033 hasConceptScore W2124633033C187206112 @default.
• W2124633033 hasConceptScore W2124633033C192396546 @default.

• next