FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2124709747> ?p ?o ?g. }

• W2124709747 endingPage "2718" @default.
• W2124709747 startingPage "2707" @default.
• W2124709747 abstract "ABSTRACT During productive herpes simplex virus type 1 (HSV-1) infection, a subset of viral delayed-early (DE) and late (L) genes require the immediate-early (IE) protein ICP27 for their expression. However, the cis -acting regulatory sequences in DE and L genes that mediate their specific induction by ICP27 are unknown. One viral L gene that is highly dependent on ICP27 is that encoding glycoprotein C (gC). We previously demonstrated that this gene is posttranscriptionally transactivated by ICP27 in a plasmid cotransfection assay. Based on our past results, we hypothesized that the gC gene possesses a cis -acting inhibitory sequence and that ICP27 overcomes the effects of this sequence to enable efficient gC expression. To test this model, we systematically deleted sequences from the body of the gC gene and tested the resulting constructs for expression. In so doing, we identified a 258-bp “silencing element” (SE) in the 5′ portion of the gC coding region. When present, the SE inhibits gC mRNA accumulation from a transiently transfected gC gene, unless ICP27 is present. Moreover, the SE can be transferred to another HSV-1 gene, where it inhibits mRNA accumulation in the absence of ICP27 and confers high-level expression in the presence of ICP27. Thus, for the first time, an ICP27-responsive sequence has been identified in a physiologically relevant ICP27 target gene. To see if the SE functions during viral infection, we engineered HSV-1 recombinants that lack the SE, either in a wild-type (WT) or ICP27-null genetic background. In an ICP27-null background, deletion of the SE led to ICP27-independent expression of the gC gene, demonstrating that the SE functions during viral infection. Surprisingly, the ICP27-independent gC expression seen with the mutant occurred even in the absence of viral DNA synthesis, indicating that the SE helps to regulate the tight DNA replication-dependent expression of gC." @default.
• W2124709747 created "2016-06-24" @default.
• W2124709747 creator A5006457894 @default.
• W2124709747 creator A5012318392 @default.
• W2124709747 creator A5039793753 @default.
• W2124709747 creator A5060792296 @default.
• W2124709747 creator A5060922456 @default.
• W2124709747 creator A5074019195 @default.
• W2124709747 date "2010-03-15" @default.
• W2124709747 modified "2023-10-15" @default.
• W2124709747 title "Identification of an ICP27-Responsive Element in the Coding Region of a Herpes Simplex Virus Type 1 Late Gene" @default.
• W2124709747 cites W1514981056 @default.
• W2124709747 cites W1540296073 @default.
• W2124709747 cites W1559441251 @default.
• W2124709747 cites W1577671916 @default.
• W2124709747 cites W1583466448 @default.
• W2124709747 cites W1592653658 @default.
• W2124709747 cites W1594175085 @default.
• W2124709747 cites W1628312488 @default.
• W2124709747 cites W1733825645 @default.
• W2124709747 cites W1757069992 @default.
• W2124709747 cites W1799661214 @default.
• W2124709747 cites W1807366979 @default.
• W2124709747 cites W1890282385 @default.
• W2124709747 cites W1895304651 @default.
• W2124709747 cites W1965372133 @default.
• W2124709747 cites W1966435564 @default.
• W2124709747 cites W1971645214 @default.
• W2124709747 cites W1977820220 @default.
• W2124709747 cites W1978856165 @default.
• W2124709747 cites W1993755997 @default.
• W2124709747 cites W2003260938 @default.
• W2124709747 cites W2006979668 @default.
• W2124709747 cites W2013076780 @default.
• W2124709747 cites W2014001404 @default.
• W2124709747 cites W2019475067 @default.
• W2124709747 cites W2023613252 @default.
• W2124709747 cites W2023686279 @default.
• W2124709747 cites W2025726628 @default.
• W2124709747 cites W2029671705 @default.
• W2124709747 cites W2045946640 @default.
• W2124709747 cites W2047079821 @default.
• W2124709747 cites W2065677302 @default.
• W2124709747 cites W2067747544 @default.
• W2124709747 cites W2071694837 @default.
• W2124709747 cites W2088756940 @default.
• W2124709747 cites W2095757228 @default.
• W2124709747 cites W2101840360 @default.
• W2124709747 cites W2110126927 @default.
• W2124709747 cites W2116445024 @default.
• W2124709747 cites W2117645968 @default.
• W2124709747 cites W2119376048 @default.
• W2124709747 cites W2121672427 @default.
• W2124709747 cites W2123922221 @default.
• W2124709747 cites W2125137658 @default.
• W2124709747 cites W2129952868 @default.
• W2124709747 cites W2136942244 @default.
• W2124709747 cites W2141157874 @default.
• W2124709747 cites W2147461226 @default.
• W2124709747 cites W2148633495 @default.
• W2124709747 cites W2153117746 @default.
• W2124709747 cites W2156805103 @default.
• W2124709747 cites W2164152668 @default.
• W2124709747 cites W2168667993 @default.
• W2124709747 doi "https://doi.org/10.1128/jvi.02005-09" @default.
• W2124709747 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2826072" @default.
• W2124709747 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20042503" @default.
• W2124709747 hasPublicationYear "2010" @default.
• W2124709747 type Work @default.
• W2124709747 sameAs 2124709747 @default.
• W2124709747 citedByCount "13" @default.
• W2124709747 countsByYear W21247097472012 @default.
• W2124709747 countsByYear W21247097472013 @default.
• W2124709747 countsByYear W21247097472014 @default.
• W2124709747 countsByYear W21247097472015 @default.
• W2124709747 countsByYear W21247097472017 @default.
• W2124709747 countsByYear W21247097472019 @default.
• W2124709747 countsByYear W21247097472021 @default.
• W2124709747 crossrefType "journal-article" @default.
• W2124709747 hasAuthorship W2124709747A5006457894 @default.
• W2124709747 hasAuthorship W2124709747A5012318392 @default.
• W2124709747 hasAuthorship W2124709747A5039793753 @default.
• W2124709747 hasAuthorship W2124709747A5060792296 @default.
• W2124709747 hasAuthorship W2124709747A5060922456 @default.
• W2124709747 hasAuthorship W2124709747A5074019195 @default.
• W2124709747 hasBestOaLocation W21247097471 @default.
• W2124709747 hasConcept C104317684 @default.
• W2124709747 hasConcept C119056186 @default.
• W2124709747 hasConcept C150194340 @default.
• W2124709747 hasConcept C153911025 @default.
• W2124709747 hasConcept C165864922 @default.
• W2124709747 hasConcept C21592294 @default.
• W2124709747 hasConcept C2522874641 @default.
• W2124709747 hasConcept C2781196997 @default.
• W2124709747 hasConcept C54355233 @default.
• W2124709747 hasConcept C86803240 @default.
• W2124709747 hasConcept C91779695 @default.
• W2124709747 hasConceptScore W2124709747C104317684 @default.

• next