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• W2124742274 abstract "Xylene oxygenase, which is encoded on the TOL plasmid pWWO of Pseudomonas putida mt-2, is a key enzyme system in the degradation of toluene and xylenes by this organism. It was expressed in an Escherichia coli recombinant strain carrying the xylMA structural genes. This recombinant, which expressed xylene oxygenase from the heat-shock induced lambda PL promoter, was analyzed for its potential as a biocatalytic tool so as to effect the oxidation of side chains of aromatic hydrocarbons to the corresponding alcohols. Compounds that were tested as potential substrates carried different substituents on the aromatic ring at ortho, meta, and para positions, relative to the methyl moiety. Products that accumulated after administration of the aromatic hydrocarbons to concentrated suspensions of the recombinant were identified by gas chromatography and mass spectrometry. Toluene derivatives with ortho substituents could not serve as substrates for the biocatalyst, whereas a number of meta- or para- substituted analogs were efficiently oxidized to the corresponding benzylalcohols. Bioconversion of the substrates by resting cells varied from 3 mumol min-1 g-1 cell dry weight for 1,3,5-trimethylbenzene to 18 mumol min-1 g-1 cell dry weight for meta-xylene. Whole cells that expressed xylene oxygenase did catalyze the oxidation of the methyl substituent attached to a benzene ring, but no conversion of n-alkylbenzene derivatives with longer side chains was observed. Although the ethyl group of ethylbenzene could not be converted by the biocatalyst, cells containing xylene oxygenase were capable of oxidizing the ethylene side group of styrene to produce styrene epoxide." @default.
• W2124742274 created "2016-06-24" @default.
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• W2124742274 date "1994-07-01" @default.
• W2124742274 modified "2023-10-18" @default.
• W2124742274 title "TOL plasmid-specified xylene oxygenase is a wide substrate range monooxygenase capable of olefin epoxidation" @default.
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• W2124742274 doi "https://doi.org/10.1016/0141-0229(94)90127-9" @default.
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