Matches in SemOpenAlex for { <https://semopenalex.org/work/W2124764306> ?p ?o ?g. }
- W2124764306 endingPage "548" @default.
- W2124764306 startingPage "535" @default.
- W2124764306 abstract "An antiserum to the beta 2 subunit of the rat gamma-aminobutyric acid (GABAA) receptor was prepared by immunizing a rabbit with a fusion protein expressed in bacteria. The fusion protein had the large, intracellular loop expanding between the putative M3 and M4 transmembrane domains of the beta 2 subunit fused to staphylococcal protein A (SPA). The antiserum immunoprecipitated both the solubilized and the affinity-purified GABAA receptors. The anti-beta 2 antibodies were affinity purified on immobilized beta 2 intracellular loop peptide. The antibodies recognized a 55-57 kDa peptide in immunoblots of either crude membranes from rat cerebral cortex or affinity-purified GABAA receptors from bovine cerebral cortex. Immunocytochemistry with the affinity-purified antibody has revealed for the first time the localization of the beta 2 subunit in the rat brain. A comparative study of the regional and cellular immunoreactivities of the affinity-purified anti-beta 2 antibody and the monoclonal antibody 62-3G1 (which recognizes both beta 2 and beta 3 subunits) is presented. The procedure described for generating and preparing specific anti-beta 2 subunit antibodies that are valuable for immunocytochemistry could be extended to other GABAA receptor subunits." @default.
- W2124764306 created "2016-06-24" @default.
- W2124764306 creator A5004693289 @default.
- W2124764306 creator A5010469690 @default.
- W2124764306 creator A5021384155 @default.
- W2124764306 creator A5043605173 @default.
- W2124764306 creator A5088168529 @default.
- W2124764306 date "1999-11-01" @default.
- W2124764306 modified "2023-10-14" @default.
- W2124764306 title "Immunocytochemical localization of the ?3 subunit of the ?-aminobutyric acidA receptor in the rat brain" @default.
- W2124764306 cites W1504992746 @default.
- W2124764306 cites W1505903665 @default.
- W2124764306 cites W1508300860 @default.
- W2124764306 cites W1524203275 @default.
- W2124764306 cites W1538927534 @default.
- W2124764306 cites W1570185216 @default.
- W2124764306 cites W1657090468 @default.
- W2124764306 cites W1784526106 @default.
- W2124764306 cites W1828083423 @default.
- W2124764306 cites W1947718130 @default.
- W2124764306 cites W1969283272 @default.
- W2124764306 cites W1974022934 @default.
- W2124764306 cites W1982860158 @default.
- W2124764306 cites W1993095599 @default.
- W2124764306 cites W1997437840 @default.
- W2124764306 cites W2001295380 @default.
- W2124764306 cites W2004268468 @default.
- W2124764306 cites W2006489225 @default.
- W2124764306 cites W2022885766 @default.
- W2124764306 cites W2036485889 @default.
- W2124764306 cites W2039738625 @default.
- W2124764306 cites W2044138283 @default.
- W2124764306 cites W2045133279 @default.
- W2124764306 cites W2046878980 @default.
- W2124764306 cites W2050243692 @default.
- W2124764306 cites W2056562685 @default.
- W2124764306 cites W2062575830 @default.
- W2124764306 cites W2064162330 @default.
- W2124764306 cites W2067237230 @default.
- W2124764306 cites W2077065023 @default.
- W2124764306 cites W2080506185 @default.
- W2124764306 cites W2088366911 @default.
- W2124764306 cites W2106899177 @default.
- W2124764306 cites W2112444339 @default.
- W2124764306 cites W2131931434 @default.
- W2124764306 cites W2139230578 @default.
- W2124764306 cites W2141896877 @default.
- W2124764306 cites W2156212448 @default.
- W2124764306 cites W2156268266 @default.
- W2124764306 cites W2924856415 @default.
- W2124764306 cites W4234396293 @default.
- W2124764306 cites W4317524510 @default.
- W2124764306 doi "https://doi.org/10.1002/(sici)1096-9861(19991101)413:4<535::aid-cne4>3.0.co;2-t" @default.
- W2124764306 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7884042" @default.
- W2124764306 hasPublicationYear "1999" @default.
- W2124764306 type Work @default.
- W2124764306 sameAs 2124764306 @default.
- W2124764306 citedByCount "58" @default.
- W2124764306 countsByYear W21247643062012 @default.
- W2124764306 countsByYear W21247643062013 @default.
- W2124764306 countsByYear W21247643062015 @default.
- W2124764306 countsByYear W21247643062016 @default.
- W2124764306 countsByYear W21247643062017 @default.
- W2124764306 countsByYear W21247643062018 @default.
- W2124764306 countsByYear W21247643062021 @default.
- W2124764306 crossrefType "journal-article" @default.
- W2124764306 hasAuthorship W2124764306A5004693289 @default.
- W2124764306 hasAuthorship W2124764306A5010469690 @default.
- W2124764306 hasAuthorship W2124764306A5021384155 @default.
- W2124764306 hasAuthorship W2124764306A5043605173 @default.
- W2124764306 hasAuthorship W2124764306A5088168529 @default.
- W2124764306 hasConcept C104292427 @default.
- W2124764306 hasConcept C104317684 @default.
- W2124764306 hasConcept C123894998 @default.
- W2124764306 hasConcept C134018914 @default.
- W2124764306 hasConcept C153911025 @default.
- W2124764306 hasConcept C159654299 @default.
- W2124764306 hasConcept C168258287 @default.
- W2124764306 hasConcept C170493617 @default.
- W2124764306 hasConcept C179247698 @default.
- W2124764306 hasConcept C181199279 @default.
- W2124764306 hasConcept C203014093 @default.
- W2124764306 hasConcept C35866371 @default.
- W2124764306 hasConcept C40767141 @default.
- W2124764306 hasConcept C55493867 @default.
- W2124764306 hasConcept C71829478 @default.
- W2124764306 hasConcept C86803240 @default.
- W2124764306 hasConcept C91523082 @default.
- W2124764306 hasConceptScore W2124764306C104292427 @default.
- W2124764306 hasConceptScore W2124764306C104317684 @default.
- W2124764306 hasConceptScore W2124764306C123894998 @default.
- W2124764306 hasConceptScore W2124764306C134018914 @default.
- W2124764306 hasConceptScore W2124764306C153911025 @default.
- W2124764306 hasConceptScore W2124764306C159654299 @default.
- W2124764306 hasConceptScore W2124764306C168258287 @default.
- W2124764306 hasConceptScore W2124764306C170493617 @default.
- W2124764306 hasConceptScore W2124764306C179247698 @default.
- W2124764306 hasConceptScore W2124764306C181199279 @default.