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- W2124780069 abstract "ABSTRACT Japanese encephalitis virus (JEV) is a mosquito-borne pathogenic flavivirus responsible for acute viral encephalitis in humans. The cellular entry of JEV is poorly characterized in terms of molecular requirements and pathways. Here we present a systematic study of the internalization mechanism of JEV in fibroblasts and neuroblastoma cells. To verify the roles of distinct pathways of cell entry, we used fluorescently labeled virus particles, a combination of pharmacological inhibitors, RNA interference (RNAi), and dominant-negative (DN) mutants of regulatory proteins involved in endocytosis. Our study demonstrates that JEV infects fibroblasts in a clathrin-dependent manner, but it deploys a clathrin-independent mechanism to infect neuronal cells. The clathrin-independent pathway requires dynamin and plasma membrane cholesterol. Virus binding to neuronal cells leads to rapid actin rearrangements and an intact and dynamic actin cytoskeleton, and the small GTPase RhoA plays an important role in viral entry. Immunofluorescence analysis of viral colocalization with endocytic markers showed that JEV traffics through Rab5-positive early endosomes and that release of the viral nucleocapsid occurs at the level of the early and not the late endosomes." @default.
- W2124780069 created "2016-06-24" @default.
- W2124780069 creator A5005586871 @default.
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- W2124780069 creator A5058270135 @default.
- W2124780069 creator A5065412587 @default.
- W2124780069 creator A5068866232 @default.
- W2124780069 date "2013-01-01" @default.
- W2124780069 modified "2023-10-13" @default.
- W2124780069 title "Japanese Encephalitis Virus Infects Neuronal Cells through a Clathrin-Independent Endocytic Mechanism" @default.
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- W2124780069 cites W1964764190 @default.
- W2124780069 cites W1965530119 @default.
- W2124780069 cites W1965635723 @default.
- W2124780069 cites W1966828940 @default.
- W2124780069 cites W1967965496 @default.
- W2124780069 cites W1968826282 @default.
- W2124780069 cites W1980056833 @default.
- W2124780069 cites W1982094437 @default.
- W2124780069 cites W1984653721 @default.
- W2124780069 cites W1986849641 @default.
- W2124780069 cites W1988039566 @default.
- W2124780069 cites W1989210142 @default.
- W2124780069 cites W1996037007 @default.
- W2124780069 cites W1996453362 @default.
- W2124780069 cites W2011126059 @default.
- W2124780069 cites W2015704490 @default.
- W2124780069 cites W2015752928 @default.
- W2124780069 cites W2016230098 @default.
- W2124780069 cites W2016747731 @default.
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- W2124780069 cites W2029302344 @default.
- W2124780069 cites W2030398776 @default.
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- W2124780069 cites W2032444338 @default.
- W2124780069 cites W2034332162 @default.
- W2124780069 cites W2035501441 @default.
- W2124780069 cites W2039242651 @default.
- W2124780069 cites W2039467740 @default.
- W2124780069 cites W2039794487 @default.
- W2124780069 cites W2040562690 @default.
- W2124780069 cites W2044191234 @default.
- W2124780069 cites W2051776908 @default.
- W2124780069 cites W2053406561 @default.
- W2124780069 cites W2059602019 @default.
- W2124780069 cites W2062452684 @default.
- W2124780069 cites W2066383202 @default.
- W2124780069 cites W2077175094 @default.
- W2124780069 cites W2078121570 @default.
- W2124780069 cites W2078998316 @default.
- W2124780069 cites W2079099220 @default.
- W2124780069 cites W2080927554 @default.
- W2124780069 cites W2084361478 @default.
- W2124780069 cites W2089920144 @default.
- W2124780069 cites W2093028387 @default.
- W2124780069 cites W2099051954 @default.
- W2124780069 cites W2102312968 @default.
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- W2124780069 cites W2126161876 @default.
- W2124780069 cites W2126371427 @default.
- W2124780069 cites W2129123695 @default.
- W2124780069 cites W2135325359 @default.
- W2124780069 cites W2135404068 @default.
- W2124780069 cites W2136414528 @default.
- W2124780069 cites W2137323006 @default.
- W2124780069 cites W2137960852 @default.
- W2124780069 cites W2137983400 @default.
- W2124780069 cites W2138623102 @default.
- W2124780069 cites W2139340707 @default.
- W2124780069 cites W2140442780 @default.
- W2124780069 cites W2141391862 @default.
- W2124780069 cites W2143254953 @default.
- W2124780069 cites W2148233172 @default.
- W2124780069 cites W2149707874 @default.
- W2124780069 cites W2152624614 @default.
- W2124780069 cites W2153767710 @default.
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- W2124780069 cites W2155259994 @default.
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- W2124780069 doi "https://doi.org/10.1128/jvi.01399-12" @default.
- W2124780069 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3536362" @default.
- W2124780069 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23055570" @default.
- W2124780069 hasPublicationYear "2013" @default.
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